Reliable camera motion estimation from compressed MPEG videos using machine learning approach

As an important feature in characterizing video content, camera motion has been widely applied in various multimedia and computer vision applications. A novel method for fast and reliable estimation of camera motion from MPEG videos is proposed, using support vector machine for estimation in a regression model trained on a synthesized sequence. Experiments conducted on real sequences show that the proposed method yields much improved results in estimating camera motions while the difficulty in selecting valid macroblocks and motion vectors is skipped

Synergistic Influence of Local Climate Zones and Wind Speeds on the Urban Heat Island and Heat Waves in the Megacity of Beijing, China

Large-scale modifications to urban underlying surfaces owing to rapid urbanization have led to stronger urban heat island (UHI) effects and more frequent urban heat wave (HW) events. Based on observations of automatic weather stations in Beijing during the summers of 2014–2020, we studied the interaction between HW events and the UHI effect. Results showed that the UHI intensity (UHII) was significantly aggravated (by 0.55°C) during HW periods compared to non-heat wave (NHW) periods. Considering the strong impact of unfavorable weather conditions and altered land use on the urban thermal environment, we evaluated the modulation of HW events and the UHI effect by wind speed and local climatic zones (LCZs). Wind speeds in urban areas were weakened due to the obstruction of dense high-rise buildings, which favored the occurrence of HW events. In detail, 35 HW events occurred over the LCZ1 of a dense high-rise building area under low wind speed conditions, which was much higher than that in other LCZ types and under high wind speed conditions (< 30 HW events). The latent heat flux in rural areas has increased more due to the presence of sufficient water availability and more vegetation, while the increase in heat flux in urban areas is mainly in the form of sensible heat flux, resulting in stronger UHI effect during HW periods. Compared to NHW periods, lower boundary layer and wind speed in the HW events weakened the convective mixing of air, further expanding the temperature gap between urban and rural areas. Note that LCZP type with its high-density vegetation and water bodies in the urban park area generally exhibited, was found to have a mitigating effect on the UHI, whilst at the same time increasing the frequency and duration of HW events during HW periods. Synergies between HWs and the UHI amplify both the spatial and temporal coverage of high-temperature events, which in turn exposes urban residents to additional heat stress and seriously threatens their health. The findings have important implications for HWs and UHII forecasts, as well as for scientific guidance on decision-making to improve the thermal environment and to adjust the energy structure

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

In-situ X-ray microtomography of interface between additively manufactured aluminium bronze and H13 tool steel

Directed energy deposition (DED) is an additive manufacturing process category where material is melted as it is deposited, often powder melted with a focused laser. In this work aluminium bronze was deposited onto H13 tool steel using this technique, forming a mixing zone between the two metals. This mixing zone was examined with X-ray microtomography to obtain a three dimensional perspective that is unrealizable with conventional microscopy. Both the shape of the melt pools, the microstructure within, and processing defects could be discerned due to varying absorption of the radiation. In addition to characterizing the microstructure, the sample was also strained in three steps; the first step was to approximately 2.5 % strain, the second to 10.5 % and finally until fracture of the sample. The sample was scanned between each step, including a scan of the fracture surface. The ultimate tensile strength was found to be approximately 850 MPa and the fracture was observed to originate from cracks between the H13 substrate and the mixing zone. These cracks appeared to form in the second step of the straining. Additionally, local strains were estimated by utilizing pores in the sample as tracking points.publishedVersio

Metagenomics-Based Analysis of the Effect of Rice Straw Substitution for a Proportion of Whole-Plant Corn Silage on the Rumen Flora Structure and Carbohydrate-Active Enzymes (CAZymes)

The purpose of this study was to investigate the effects of replacing a portion of whole-plant corn silage with straw on the rumen microbial community structure and carbohydrate-active enzyme activity. The experiment employed a single-factor randomized trial design, with eight late-lactation Chinese Holstein dairy cows being randomly divided into two groups of four replicates each. The control group (CS group) was fed a diet consisting of alfalfa silage and a mixture of alfalfa and whole-plant corn silage, while the experimental group (RS group) received a diet in which one-third of the corn silage was replaced with straw while keeping the other components unchanged. The experiment lasted for a total of 21 days, with a pre-feeding period of 14 days and a formal period of 7 days. The rumen fluid collected on day 21 was used for the rumen fermentation parameters and metagenomic analysis. The concentrations of acetic acid, propionic acid, butyric acid, and total volatile fatty acids (TVFA) in the rumen of RS group cows were significantly lower than those in the CS group (p p Bacteroides, Alistipes, Butyrivibrio, Chlamydia, Fibrobacter, unclassified Ruminococcaceae, and unclassified Bacteroidetes in the rumen of RS group cows increased, while the abundance of Eubacterium decreased ([LDA > 3.6], p p Alistipes, Bacteroides, and Butyrivibrio showed a significant negative correlation with propionic acid (p p p < 0.05). In summary, straw can not only alter the composition and structure of the rumen microbiota in cows but also affect the relative abundance of CAZymes at different levels within the rumen. Cows may, thus, potentially improve the degradation efficiency of straw diets by increasing the abundance of certain rumen microbiota and enzymes

Experimental study of the interface evolution behavior and softening mechanism of structure-marine soil

The undrained interface characteristics of marine clay-structures are key to the design of offshore platforms. However, it is difficult to acquire interfacial shear-induced pore pressure measurements employing conventional interface equipment. This paper introduces an interface instrument that is suitable for evaluating marine soil-structure interface characteristics. The developed device is utilized to investigate the undrained interfacial evolution behavior and softening mechanism of specimens with different moisture contents. The results indicate that the interface undrained strength curve exhibits high stiffness initially, and then declines as the strain level increases. The interfacial soil particles experience two stages: obvious contraction and slight dilatancy tendency. The moisture content response to interface shear resistance is mainly through changing interface adhesion. Additionally, the reduction in contact area caused by interfacial particle dilatancy is the internal inducement of interfacial strain softening. The increase in interfacial soil moisture content caused by water migration is another external inducement. Accordingly, the stress development state in the whole shear process and the variation in free water migration toward the interface are revealed, providing an excellent analytical basis for further investigation of undrained interface behavior

Evaluating Fouling Control and Energy Consumption in a Pilot-Scale, Low-Energy POREFLON Non-Aerated Membrane Bioreactor (LEP-N-MBR) System at Different Frequencies and Amplitudes

Continual aeration, a fouling control strategy that causes high energy consumption, is the major obstacle in the deployment of membrane bioreactors (MBRs) for wastewater treatment. In recent years, a technology has been developed which adopts mechanical reciprocity for membrane vibration, and it has been proven efficient for membrane scouring, as well as for saving energy: the low-energy POREFLON non-aerated membrane bioreactor (LEP-N-MBR). In this study, a pilot-scale LEP-N-MBR system was designed, established, and operated at various frequencies and amplitudes, and with various membrane models, so as to evaluate energy usage and membrane fouling. The results showed that a slower TMP rise occurred when the frequency and amplitude were set to 0.5 Hz and 10 cm, respectively. Under a suitable frequency and amplitude, the TMP increasing rate of model B (sealed only with epoxy resin) was slower than that of model A (sealed with a combination of polyurethane and epoxy resin). The average specific energy demand (SED) of the LEP-N-MBR was 0.18 kWh&middot;m&minus;3, much lower than the aerated MBR with 0.43 kWh&middot;m&minus;3 (obtained from a previous study), indicating a significant decrease of 59.54% in the SED. However, the uneven distribution of sludge within the membrane tank indicated that the poor hydraulic mixing in the reactor may result in sludge accumulation, which requires further operational optimization. The findings of this pilot-scale study suggest that the LEP-N-MBR system is promising and effective for municipal wastewater treatment with a much lower level of energy usage. More research is needed to further optimize the operation of the LEP-N-MBR for wide application