Data_Sheet_1_Sea buckthorn pulp and seed oils ameliorate lipid metabolism disorders and modulate gut microbiota in C57BL/6J mice on high-fat diet.docx

IntroductionNon-alcoholic fatty liver diseases (NAFLD), along with the complications of obesity and dyslipidemia, are worldwide lipid metabolism disorders. Recent evidence showed that NAFLD could be ameliorated by diet and lifestyles by attenuating gut microbiota dysbiosis via the gut–liver axis. Sea buckthorn oils, including sea buckthorn pulp oil (SBPO) and sea buckthorn seed oil (SBSO), were investigated in this study for their beneficial effects on gut–liver axis in C57BL/6J mice on a high-fat diet.MethodsSixty of male C57BL/6J mice were assigned into five groups, fed with low-fat diet containing soybean oil (SO), high-fat diet comprising lard oil (LO), peanut oil (PO), SBSO or SBPO, respectively, for 12 weeks. Serum and hepatic biochemical analysis, liver and perirenal fat histological analysis, and fecal 16S rRNA gene sequencing were conducted to reflect the influence of five diets on gut-liver axis.ResultsDietary SBPO reduced visceral fat accumulation, adipose cell size, serum and hepatic triglyceride, LDL-C levels, and hepatic cell damage score; increased gut microbiota diversity with a higher abundance of Lactobacillus, Roseburia, and Oscillibacter compared with PO. SBSO showed equal or weaker effects compared to SBPO.ConclusionThis study demonstrates that dietary SBPO has the potential to ameliorate NAFLD and related metabolic disorders, like obesity and dyslipidemia, by modulating gut microbiota.</p

Formation of 3‑Monochloro-1,2-propanediol (3-MCPD) Di- and Monoesters from Tristearoylglycerol (TSG) and the Potential Catalytic Effect of Fe²⁺ and Fe³⁺

This study investigated whether and how triacylglycerol (TAG) may serve as a precursor for 3-monochloro-1,2-propanediol (3-MCPD) fatty acid ester formation using tristearoylglycerol (TSG). TSG was reacted with inorganic chloride compounds including NaCl, KCl, FeCl₂, CuCl_2, ZnCl₂, FeCl₃ and dry HCl, or organic chlorine compound lindane at different temperatures. Only FeCl₂ and FeCl₃ were able to form 3-MCPD esters from TSG. Further electron spin resonance (ESR) determination of TSG, Fe₂(SO₄)₃ and 5,5-dimethylpyrroline-<i>N</i>-oxide (DMPO) reactions revealed potential of Fe ion in promoting free radical generations under the experimental conditions. To further confirm the effect of Fe ion, chelating agent (EDTA-2Na) was added to the model reactions. The results showed for the first time that EDTA-2Na was able to reduce the generation of 3-MCPD esters. In addition, FT-IR examination indicated a possible involvement of a carbonyl group during the reaction. Taking all the observations together, the possible mechanisms, involving the formation of either a cyclic acyloxonium or a glycidol ester radical intermediate, were proposed for generating 3-MCPD fatty acid di- and mono- esters from TAG under a high temperature and low moisture condition, as well as the coformation of glycidol esters. The results from this study may be useful for reducing the level of 3-MCPD esters and related toxicants in the refined edible oils and food products

Effects of Medium- and Long-Chain Triacylglycerols on Lipid Metabolism and Gut Microbiota Composition in C57BL/6J Mice

Obesity is related to an increasing risk of chronic diseases. Medium- and long-chain triacylglycerols (MLCT) have been recognized as a promising choice to reduce body weight. In this study, three MLCT with different contents of medium-chain fatty acids (MCFA) (10–30%, w/w) were prepared, and their effects on lipid metabolism and fecal gut microbiota composition of C57BL/6J mice were systematically investigated. MLCT with 30% (w/w) MCFA showed the best performance in decreasing body weight gain as well as optimizing serum lipid parameters and liver triacylglycerol content. The expression levels of genes encoding enzymes for fatty acid degradation increased markedly and expression levels of genes encoding enzymes for <i>de novo</i> fatty acid biosynthesis decreased significantly in the liver of mice treated with MLCT containing 30% (w/w) MCFA. Interestingly, the dietary intake of a high fat diet containing MLCT did significantly decrease the ratio of <i>Firmicutes</i> to <i>Bacteroidetes</i> and down-regulate the relative abundance of <i>Proteobacteria</i> that may attribute to weight loss. Furthermore, we found a notable increase in the total short-chain fatty acid (SCFA) content in feces of mice on a MLCT containing diet. All these results may be concomitantly responsible for the antiobesity effect of MLCT with relatively high contents of MCFA