Additional file 1: Table S1. of Prenatal levonorgestrel exposure induces autism-like behavior in offspring through ERβ suppression in the amygdala

Sequences of primers for the real-time quantitative PCR (qPCR).Table S2. mRNA level by qPCR in the hypothalamus, hippocampus, and amygdala from 10 weeks old offspring. Figure S1. Prenatal levonorgestrel exposure does not affect the expression of ERβ and its target genes in the hypothalamus and hippocampus in 10-week-old offspring. Three-month-old pregnant dams were exposed to LNG (20 μg levonorgestrel), EE (10 μg ethinyl estradiol), LNG/EE (20 μg LNG and 10 μg EE combination), or VEH (vehicle, 5% ethanol in organic sesame oil) by subcutaneous daily injection of 0.1 ml for 21 days until pup delivery. Both male and female offspring were sacrificed at 10 weeks old to isolate the hypothalamus and hippocampus tissues for further analysis. (a–c) The mRNA levels in the hypothalamus for genes of ERβ (a), SOD2 (b), and ERRα (c), n = 5. (d–f) The mRNA levels in the hippocampus for genes of ERβ (d), SOD2 (e), and ERRα (f), n = 5. Results are expressed as mean ± SEM. Data S1 Statistical details for Fig. 1. Data S2 Statistical details for Fig. 2. Data S3 Statistical details for Fig. 3. Data S4 Statistical details for Fig. 5. (DOCX 63 kb