Will Jargon Use Increase or Decrease a Doctor\u27s Credibility? Exploring the Moderating Effects of eHealth Literacy and Question Type

Doctors are accustomed to using jargon to communicate in online medical communities, but is it actually effective? In this article, we propose two diametrically opposed mechanisms of jargon use that affect patients\u27 confidence in providers of online medical consultation services: The use of jargon affects competence-based confidence positively, but negatively on benevolence- and integrity-based trust. We take into account the moderating effects of eHealth literacy and question type to better comprehend the circumstances in which jargon use is at play. To test our conceptual model, we conduct a scenario experiment and then use a survey method to collect 203 valid questionnaires. Finally, we discuss our findings, their implications for theory and practise, and the study\u27s limitations

De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia Lipolytica

<p>Abstract</p> <p>Background</p> <p>Conjugated linoleic acid (CLA) has many well-documented beneficial physiological effects. Due to the insufficient natural supply of CLA and low specificity of chemically produced CLA, an effective and isomer-specific production process is required for medicinal and nutritional purposes.</p> <p>Results</p> <p>The linoleic acid isomerase gene from <it>Propionibacterium acnes</it> was expressed in <it>Yarrowia lipolytica</it> Polh. Codon usage optimization of the PAI and multi-copy integration significantly improved the expression level of PAI in <it>Y. lipolytica.</it> The percentage of <it>trans</it>-10, <it>cis</it>-12 CLA was six times higher in yeast carrying the codon-optimized gene than in yeast carrying the native gene. In combination with multi-copy integration, the production yield was raised to approximately 30-fold. The amount of <it>trans</it>-10, <it>cis</it>-12 CLA reached 5.9% of total fatty acid yield in transformed <it>Y. lipolytica</it>.</p> <p>Conclusions</p> <p>This is the first report of production of <it>trans</it>-10, <it>cis</it>-12 CLA by the oleaginous yeast <it>Y. lipolytica</it>, using glucose as the sole carbon source through expression of linoleic acid isomerase from <it>Propionibacterium acnes</it>.</p

Planar carbon nanotube-graphene hybrid films for high-performance broadband photodetectors

Graphene has emerged as a promising material for photonic applications fuelled by its superior electronic and optical properties. However, the photoresponsivity is limited by the low absorption cross section and ultrafast recombination rates of photoexcited carriers. Here we demonstrate a photoconductive gain of $\sim$ 10$^5$ electrons per photon in a carbon nanotube-graphene one dimensional-two dimensional hybrid due to efficient photocarriers generation and transport within the nanostructure. A broadband photodetector (covering 400 nm to 1550 nm) based on such hybrid films is fabricated with a high photoresponsivity of more than 100 AW$^{-1}$ and a fast response time of approximately 100 {\mu}s. The combination of ultra-broad bandwidth, high responsivities and fast operating speeds affords new opportunities for facile and scalable fabrication of all-carbon optoelectronic devices.Comment: 21 pages, 3 figure

L-band GHz femtosecond passively harmonic mode-locked Er-doped fiber laser based on nonlinear polarization rotation

Via using an L-band optimized in-fiber polarizing grating device, a GHz L-band femtosecond passively harmonic mode-locked (PHML) Er-doped fiber laser based on nonlinear polarization rotation (NPR) is firstly demonstrated. 4.22 GHz pulses with the duration of 810 fs and super-mode suppression ratio (SMSR) of 32 dB are obtained under the pump power of 712 mW corresponding to 215th harmonic order. The central wavelength of 4.22 GHz pulses is 1581.7 nm with 10.1 nm 3-dB bandwidth. Furthermore, under this fixed pump power, higher harmonic orders can also be attained by rotating the polarization controllers (PCs) properly. The highest repetition rate we obtained is 7.41 GHz with the SMSR of 20.7 dB

EHRAgent: Code Empowers Large Language Models for Few-shot Complex Tabular Reasoning on Electronic Health Records

Large language models (LLMs) have demonstrated exceptional capabilities in planning and tool utilization as autonomous agents, but few have been developed for medical problem-solving. We propose EHRAgent, an LLM agent empowered with a code interface, to autonomously generate and execute code for multi-tabular reasoning within electronic health records (EHRs). First, we formulate an EHR question-answering task into a tool-use planning process, efficiently decomposing a complicated task into a sequence of manageable actions. By integrating interactive coding and execution feedback, EHRAgent learns from error messages and improves the originally generated code through iterations. Furthermore, we enhance the LLM agent by incorporating long-term memory, which allows EHRAgent to effectively select and build upon the most relevant successful cases from past experiences. Experiments on three real-world multi-tabular EHR datasets show that EHRAgent outperforms the strongest baseline by up to 29.6% in success rate. EHRAgent leverages the emerging few-shot learning capabilities of LLMs, enabling autonomous code generation and execution to tackle complex clinical tasks with minimal demonstrations.Comment: Work in Progres

Effects of Simulated Microgravity on Embryonic Stem Cells

There have been many studies on the biological effects of simulated microgravity (SMG) on differentiated cells or adult stem cells. However, there has been no systematic study on the effects of SMG on embryonic stem (ES) cells. In this study, we investigated various effects (including cell proliferation, cell cycle distribution, cell differentiation, cell adhesion, apoptosis, genomic integrity and DNA damage repair) of SMG on mouse embryonic stem (mES) cells. Mouse ES cells cultured under SMG condition had a significantly reduced total cell number compared with cells cultured under 1 g gravity (1G) condition. However, there was no significant difference in cell cycle distribution between SMG and 1G culture conditions, indicating that cell proliferation was not impaired significantly by SMG and was not a major factor contributing to the total cell number reduction. In contrast, a lower adhesion rate cultured under SMG condition contributed to the lower cell number in SMG. Our results also revealed that SMG alone could not induce DNA damage in mES cells while it could affect the repair of radiation-induced DNA lesions of mES cells. Taken together, mES cells were sensitive to SMG and the major alterations in cellular events were cell number expansion, adhesion rate decrease, increased apoptosis and delayed DNA repair progression, which are distinct from the responses of other types of cells to SMG

Genome Characterization of the Oleaginous Fungus Mortierella alpina

Mortierella alpina is an oleaginous fungus which can produce lipids accounting for up to 50% of its dry weight in the form of triacylglycerols. It is used commercially for the production of arachidonic acid. Using a combination of high throughput sequencing and lipid profiling, we have assembled the M. alpina genome, mapped its lipogenesis pathway and determined its major lipid species. The 38.38 Mb M. alpina genome shows a high degree of gene duplications. Approximately 50% of its 12,796 gene models, and 60% of genes in the predicted lipogenesis pathway, belong to multigene families. Notably, M. alpina has 18 lipase genes, of which 11 contain the class 2 lipase domain and may share a similar function. M. alpina's fatty acid synthase is a single polypeptide containing all of the catalytic domains required for fatty acid synthesis from acetyl-CoA and malonyl-CoA, whereas in many fungi this enzyme is comprised of two polypeptides. Major lipids were profiled to confirm the products predicted in the lipogenesis pathway. M. alpina produces a complex mixture of glycerolipids, glycerophospholipids and sphingolipids. In contrast, only two major sterol lipids, desmosterol and 24(28)-methylene-cholesterol, were detected. Phylogenetic analysis based on genes involved in lipid metabolism suggests that oleaginous fungi may have acquired their lipogenic capacity during evolution after the divergence of Ascomycota, Basidiomycota, Chytridiomycota and Mucoromycota. Our study provides the first draft genome and comprehensive lipid profile for M. alpina, and lays the foundation for possible genetic engineering of M. alpina to produce higher levels and diverse contents of dietary lipids

Advances of Treatment about Elderly Clinical Stage I Non-small Cell Lung Cancer

With the aging of the population, the proportion of elderly lung cancer is increasing. More than ten years, lobectomy with mediastinal lymph node dissection has been the standard surgery for stage I non-small cell lung cancer (NSCLC). However, recent studies found that sublobectomy could get the same long-term outcome with the lobectomy for elderly clinical stage I NSCLC and more normal lung tissue could be retained. It becomes controversial again about the standard surgery of stage I NSCLC at present. Elderly stage I NSCLC is a special group, who often can not tolerate thoracotomy because of the poor body function and some comorbidities, but the thoracoscopic surgery and the stereotactic radiotherapy technology (STRT) supply them more options. The treatment of eldly stage I NSCLC is developing towards to the individualization and diversification

Supplemental Stability Criteria with New Formulation for Linear Time-Invariant Fractional-Order Systems

In this paper, new stability criteria for linear time-invariant fractional-order systems (LTIFOSs) based on linear matrix inequalities (LMIs) are derived. The solved variable of the existing LMI formulations is generalized to a complex one. In addition, based on the congruent transformation, a new LMI formulation is obtained, which is different from those in the existing literature. To deal with the above LMIs more conveniently with simulation software, the complex LMIs are converted to equivalent real LMIs. Finally, numerical examples are presented to validate the effectiveness of our theoretical results