207Pb and 17O NMR Study of the Electron Density Distribution in Metal Phase of BaPb_{1-x}Bi_xO_3

The 17O and 207Pb NMR spectra were measured in ceramic samples in the metallic phase of BaPb_{1-x}Bi_{x}O_3 oxides (0<x< 0.33). The inhomogeneous magnetic broadening which appears due to a distribution of the Knight shifts was analyzed in detail. It is shown that Bi atoms, which are randomly incorporated in BaPbO_3 parent compound give rise to an increased conduction electron spin density within an area which is delimited by its two first cation shells. According to NMR data the percolative overlap of these areas occurs in superconducting compositions and it is accompanied by a sharp growth of the average Knight shift . The decrease of with temperature revealed for x=0.33 evidences for an opening of the energy gap near E_F near the metal-semiconductor transition (x=0.35).Comment: submitted to Phys. Rev.

Analysis of the volume of medical services

The article deals with the market of paid medical services. The aim of the work is to analyze the volume of medical services in the city of Yekaterinburg in the study period from 2016 to 2020 and in Russia as a whole. This article will be of interest to people working and related to healthcare.В статье идет речь о рынке платной медицинской услуги. Целью работы является: анализ объёма медицинских услуг в городе Екатеринбург в исследуемый период с 2016 по 2020 гг. и в России в целом. Данная статья будет интересна, людям, работающим и связанных с здравоохранением

“Usage a CRISPR/Cas9-based for obtaining knockouts of economically significant cattle genes”

An experimental work dealing with the gene modification using the Cas9 RNA-based editing system was performed. Point site-specific breakpoints in gDNA were introduced at the zygote stage by microinjection of spCas9 mRNA protein and guide RNAs into the zygote cytoplasm. Oocytes that extruded the first and second polar bodies were used for the injection. 2 series of microinjections of gene editing designs for early bovine embryos were made. The degeneration ranged from 10% to 56% in different groups. A total of 100 injections were performed. Cleavage was started by 78% of the surviving oocytes; 5 embryos reached the blastocyst stage, which was 16% of the number of dividing embryos. All the resulting embryos were analyzed to evaluate the efficiency of editing. gDNA was isolated from all embryos that had reached the blastocyst stage. Using Sanger sequencing of genes of interest in pre-implantation bovine embryos and biopsies from them, it was shown that in 5 out of 17 embryos resulting from microinjections of guide RNA against the BLG gene and SpCas9 mRNA, and in 2 out of 9 embryos after microinjections of guide RNA against CD209 gene and SpCas9 mRNA, the required genome modifications were found. This is indicative of the high efficiency of this delivery method of the editing system

Hemorrhagic Fever with Renal Syndrome (History, problems and Research Perspectives)

The work is devoted to clarify the etiological aspects of the hemorrhagic fever with renal syndrome (HFRS), clinical and epidemiological features that are closely linked to the etiology, as well as to development of methods and products for specific diagnosis and vaccine prevention of hantavirus infection. That regard to hantaviruses a set of virological, immunological and molecular-genetics methods were developed significantly enhance the effectiveness of the specific diagnostics of HFRS. The features of humoral immunity in HFRS were identified and atypical clinical forms of HFRS was istablished; indicators of natural immunity to hantavirus in the population living in different regions of Russia and the former Soviet republics have been identified that allowed us to refine nosological area of HFRS; new, previously unknown natural foci of HFRS, including hantavirus RNA, in the central regions of Russia and in the subtropical zone of Krasnodar region have been identified and studied; 76 strains and 70 isolates of 9 rodent species and one species of birds, as well as from the blood of patients with HFRS and sectional materials from HFRS dead patients have been isolated and identified; new hantavirus species - Khabarovsk, Taimyr-Topografov, Adler, as well as two new genotypes Dobrava/Belgrad virus - Kurkino and Sochi have been identified; species composition of small mammals - the natural reservoir of hantaviruses was the refined; etiological role and epidemiological importance of different hantaviruse types in HFRS incidence structure was established; the existence of new etiologically distinct hantavirus infections with significant epidemiological differences has been proven; manufacturing techniques and methods of control of the culture inactivated vaccine against HFRS has been developed