Self-fertile cybrids nicotiana tabacum (+hyoscyamus aureus) with a nucleo-plastome incompatibility

Cytoplasmic hybrids (cybrids) in a novel inter-generic combination, Nicotiana tabacum (+Hyoscyamus aureus), were generated by fusion of protoplasts from a plastome tobacco albino mutant (line R100a1) and %-irradiated green protoplasts of H. aureus. Cybrids possessed a plastome of H. aureus and a rearranged mitochondrial DNA. The cybrids displayed a syndrome of nucleo-plastome incompatibility expressed as a partial chlorophyll-deficiency of cotyledonary and true leaves at the early stage of vegetative development of plants grown from seeds in soil. During later development, the plants restored a normal green coloration. This character is phenotypically indistinguishable from the same syndrome in previously generated cybrids N. tabacum (+H. nigrum). In contrast to the cybrids N. tabacum (+H. nigrum), cybrids N. tabacum (+H. aureus) were self-fertile, and did not manifest other features that were interpreted as nucleo-mitochondrial incompatibilities in N. tabacum (+H. nigrum) plants. Therefore, the cybrids N. tabacum (+H. aureus) present a self-propagating system of Nicotiana (+Hyoscyamus) nucleo-plastome incompatibility in its pure form

Підкріплення отворів в оболонках пружно деформуються кільцями

Глеба, А. Ю. Подкрепление отверстий в оболочках упругодеформированными кольцами : автореф. дис. … канд. техн. наук: 01.02.03 / А. Ю. Глеба ; Днепропетр. ин-т инж. ж.-д. трансп. — Днепропетровск, 1983. — 18 с. Защита — 17 июня 1983 г.RU: Исследуются соотношения, описывающие напряженно-деформированное состояние сферической и цилиндрической оболочек, ослабленных круговыми отверстиями, края которых подкреплены упруго-деформированными элементами.UK: Досліджуються співвідношення, що описують напружено-деформований стан сферичної і циліндричної оболонок, ослаблених круговими отворами, краї яких підкріплені пружно-деформованими елементами

New CMS-associated phenotypes in cybrids nicotiana tabacum L. (+Hyoscyamus niger L.).

Morphological characteristics were studied in cytoplasmic male sterile (CMS) cybrids possessing the tobacco nuclear genome, Hyoscyamus niger plastome and recombinant mitochondria. After backcrosses with tobacco, new flower modifications were found, including: conversions of stamens into branched filamentous structures; alterations in the shape of petals and the corolla limb; and high degrees of reduction in most flower organs. Vegetative alterations (leaf elongation and stem branching) occurred in some cybrids. Results confirmed that a protoplast fusion-based alloplasmic cytoplasm transfer, followed by conventional backcrosses, is a useful tool for generating alternative CMS sources with novel nucleo-cytoplasmic compositions. These alterations in the genetic status were accompanied by modified floral and vegetative phenotypes