9 research outputs found
Parametric All-Optical Modulation on Chip
We demonstrate parametric all-optical modulation in a periodically-poled lithium niobate microring resonator on chip. It employs quantum Zeno blockade between two distinct waves, a signal and a pump, through their sum-frequency generation at a large per-photon efficiency of 8.2 MHz. With nanosecond pump pulses at 6 mW peak power, 85.7% modulation extinction is observed, marking over 30~times efficiency improvement across various previous implementations. With only 2 mW pump peak power, 43.0% modulation extinction is observed for a doubly-stronger signal at 4 mW. This demonstrates, for the first time, that optical transistors with cascadability and fan-out are possible with just parametric nonlinear optics. These results, together with inherent advantages in such photonic integrated circuits, open the door to scalable technology for all-optical and quantum information processing
Phylogenetic neighbor-joining trees of the haloarchaeal 16S rDNA sequences.
<p>The nucleotide sequences flanking the initiation codons of their corresponding <i>hsp70</i> genes had been shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138473#pone.0138473.s001" target="_blank">S1 Fig</a>. The strains, in which nucleotides flanking the initiation codons of <i>hsp70</i> gene were not in consensus, were marked with thick lines and â—Ź.</p
Bioinformatic analysis of the nucleotide sequences flanking the start codons of <i>hsp70</i> genes from 92 sequenced haloarchaea.
<p>The sequences from -10 to +10 were downloaded from NCBI and the sequence logo was generated by Weblogo [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138473#pone.0138473.ref026" target="_blank">26</a>]. The first base of start codon was defined as position +1.</p
Start codon selectivities at haloarchaeal transcripts in the presence and absence of short 5’-UTR.
<p>(A) The 203-bp DNA sequence preceding the <i>bgaH</i> ORF in plasmid pTMJ was identical to the upstream sequence of <i>hsp70</i> ORF in <i>Natrinema</i> sp. J7. The bases at the 5’-end of transcripts were shown schematically. Their start codons were underlined and the mutations were in grey. The <i>β</i>-galactosidase specific activities (BgaH activities), the <i>bgaH</i> transcript levels and the translational efficiencies of <i>Hfx</i>. <i>volcanii</i> transformants were tabulated. (B) The translational efficiencies of (A) were shown schematically after normalization to that of <i>Hfx</i>. <i>volcanii</i> DS70/pTMJ. (C) The expression of the BgaH protein. Western blot analysis of the BgaH protein in total proteins was performed using anti-BgaH antibody. The <i>Hfx</i>. <i>volcanii</i> transformants were cultivated for 5 days at 45°C and then sprayed with X-Gal. The constructs present in each transformant were indicated under the colonies.</p
The effects of the bases in 5’-UTR other than -3A on gene expression.
<p>(A) The bases at the 5’-end of transcripts were shown schematically and the mutation bases were in grey. The <i>β</i>-galactosidase specific activities (BgaH activities), the <i>bgaH</i> transcript levels and the translational efficiencies in recombinant strains were tabulated. (B) The translational efficiencies of (A) were shown schematically after normalization to that of <i>Hfx</i>. <i>volcanii</i> DS70/pTMJ. (C) Colonies of <i>Hfx</i>. <i>volcanii</i> transformants sprayed with X-Gal. The constructs present in each transformant were indicated under the colonies.</p
The effect of the nucleotide at position -3 on gene expression.
<p>(A) The bases at the 5’-end of transcripts were shown schematically and the mutational bases were in grey. The <i>β</i>-galactosidase specific activities (BgaH activities), the <i>bgaH</i> transcript levels and the translational efficiencies of <i>Hfx</i>. <i>volcanii</i> transformants were tabulated. (B) The translational efficiencies of (A) were shown schematically after normalization to that of <i>Hfx</i>. <i>volcanii</i> DS70/pTMJ. (C) Colonies of <i>Hfx</i>. <i>volcanii</i> transformants sprayed with X-Gal. The strains were cultivated for 5 days and then sprayed with X-Gal. The constructs present in each transformant were indicated under the colonies.</p
The effects of the base substitutions in the penultimate amino acid codon.
<p>(A) The bases at the 5’-end of transcripts were shown schematically and the substitutions were in grey. The <i>β</i>-galactosidase specific activities (BgaH activities), the <i>bgaH</i> transcript levels and the translational efficiencies of <i>Hfx</i>. <i>volcanii</i> strains were tabulated. (B) The translational efficiencies of (A) were shown schematically after normalization to that of <i>Hfx</i>. <i>volcanii</i> DS70/pTMJ. (C) Colonies of <i>Hfx</i>. <i>volcanii</i> transformants sprayed with X-Gal. The constructs present in each transformant were indicated under the colonies.</p
Molar Range Detection Based on Sideband Differential Absorption Spectroscopy with a Concentrated Reference
Conventional absorption
spectroscopy (CAS) with a blank reference
has only a slight capacity to detect high concentrations at characteristic
wavelengths owing to the corresponding large molar absorption coefficient
(ε) on the scale of 10<sup>3</sup> or 10<sup>4</sup> cm<sup>–1</sup> M<sup>–1</sup>. To monitor concentrated analytes
as high as the molar range in a plating bath and on a chemical production
line, we propose a new approach using sideband differential absorption
spectroscopy (SDAS). SDAS is obtained by subtracting the absorption
spectra of the samples, <i>A</i>(λ,<i>C</i><sub><i>x</i></sub>), from that of a reference containing
a concentrated standard analyte, <i>A</i>(λ,<i>C</i><sub>ref</sub>><i>C</i><sub><i>x</i></sub>), resulting in concave spectra with peaks at the sideband
of conventional spectra with generally low ε values on the scale
of 100 cm<sup>–1</sup> M<sup>–1</sup> or less. The negative
absorbance changes linearly with the sample concentration at a certain
peak wavelength, obeying Lambert–Beer’s law. In this
work, SDAS was obtained and verified using inorganic and organic substances,
such as chromate potassium, rhodamine B, and paracetamol
Additional file 1: Figure S1. of Epidemiology of invasive group B streptococcal disease in infants from urban area of South China, 2011–2014
Partial sequence diagram for seven house-keeping genes. adhP gene(A), pheS gene(B), atr gene(C), glnA gene(D), sdhA gene(E), glcK gene(F), tkt gene(G). (DOCX 849 kb