Anti-inflammatory Lanostanoids and Lactone Derivatives from <i>Antrodia camphorata</i>

Four new lanostanoids, ethyl lucidenate A (<b>1</b>), ethyl lucidenate F (<b>2</b>), 15-<i>O</i>-acetylganolucidate A (<b>3</b>), and 3,11,15,23-tetraoxo-27ξ-lanosta-8,16-dien-26-oic acid (<b>4</b>), and two new lactone derivatives, 5-hydroxy-5-(methoxymethyl)-4-methylfuran-2­(5<i>H</i>)-one (<b>5</b>) and 3-(4-methoxy-2-oxo-2<i>H</i>-pyran-6-yl)­propanoic acid (<b>6</b>), together with four known compounds, 11α-hydroxy-3,7-dioxolanost-8,24­(<i>E</i>)-dien-26- oic acid (<b>7</b>), 3,7,11-trioxo-5α-lanosta-8,24­(<i>E</i>)-dien-26-oic acid (<b>8</b>), methyl 3,7,11,12,15,23-hexaoxo-5α-lanost-8-en-26-oate (<b>9</b>), and ethyl 3,7,11,12,15,23-hexaoxo-5α-lanost-8-en-26-oate (<b>10</b>), were characterized from <i>Antrodia camphorata</i>. The structures of these new compounds were determined by analysis of their spectroscopic data, including 1D and 2D NMR experiments. Ten components were evaluated for anti-inflammatory activity by examining their effect on LPS-iNOS-dependent NO production in murine macrophage (RAW 264.7) cells. Among them, compounds <b>1</b>, <b>3</b>, <b>7</b>, <b>8</b>, <b>9</b>, and <b>10</b> significantly suppressed the NO concentration in LPS-treated RAW 264.7 cells with IC₅₀ values ≤ 10 μM