Factor Analysis of Low-Frequency Repetitive Transcranial Magnetic Stimulation to the Temporoparietal Junction for Tinnitus

Objectives. We investigated factors that contribute to suppression of tinnitus after repetitive transcranial magnetic stimulation (rTMS). Methods. A total of 289 patients with tinnitus underwent active 1 Hz rTMS in the left temporoparietal region. A visual analog scale (VAS) was used to assess tinnitus loudness. All participants were interviewed regarding age, gender, tinnitus duration, laterality and pitch, audiometric parameters, sleep, and so forth. The resting motor thresholds (RMTs) were measured in all patients and 30 age-and gender-matched volunteers. Results. With respect to different factors that contribute to tinnitus suppression, we found improvement in the following domains: shorter duration, normal hearing (OR: 3.25, 95%CI: 2.01-5.27, = 0.001), and without sleep disturbance (OR: 2.51, 95%CI: 1.56-4.1, = 0.005) adjusted for age and gender. The patients with tinnitus lasting less than 1 year were more likely to show suppression of tinnitus (OR: 2.77, 95%CI: 1.48-5.19, = 0.002) compared to those with tinnitus lasting more than 5 years. Tinnitus patients had significantly lower RMTs compared with healthy volunteers. Conclusion. Active low-frequency rTMS results in a significant reduction in the loudness of tinnitus. Significant tinnitus suppression was shown in subjects with shorter tinnitus duration, with normal hearing, and without sleep disturbance

SARS-CoV Infection in a Restaurant from Palm Civet

Contact with food animals was associated with SARS-CoV infection in the People’s Republic of China

Global diversity and biogeography of potential phytopathogenic fungi in a changing world

Phytopathogenic fungi threaten global food security but the ecological drivers of their global diversity and biogeography remain unknown. Here, we construct and analyse a global atlas of potential phytopathogenic fungi from 20,312 samples across all continents and major oceanic island regions, eleven land cover types, and twelve habitat types. We show a peak in the diversity of phytopathogenic fungi in mid-latitude regions, in contrast to the latitudinal diversity gradients observed in aboveground organisms. Our study identifies climate as an important driver of the global distribution of phytopathogenic fungi, and our models suggest that their diversity and invasion potential will increase globally by 2100. Importantly, phytopathogen diversity will increase largely in forest (37.27-79.12%) and cropland (34.93-82.51%) ecosystems, and this becomes more pronounced under fossil-fuelled industry dependent future scenarios. Thus, we recommend improved biomonitoring in forests and croplands, and optimised sustainable development approaches to reduce potential threats from phytopathogenic fungi

Analysis of GNSS Postseismic Deformation of Wenchuan Earthquake

Processing and analysis of 109 GNSS continuous stations and campaign stations after Wenchuan earthquake on both sides of the Longmen shan fault during the year of 1999-2015,we use the international high-precision data processing software to obtain the time series of horizonal coordinate for each site.Considering the effect of coseismic deformation of Lushan earthquake in 2013 and performing time series analysis,get the postseismic deformation field of 109 stations in the earthquake area during 2010-2015,maximum postseismic displacement in horizontal up to 5~7 cm in the epicenter near the upper falut.Based on the model of postseismic viscoelastic relaxation and searched by grid,inverted the best thickness of the middle to upper crustal elastic layer and the best coefficient of viscosity in viscoelastic layer of lower crust and upper mantle according to three different rupture models,and obtained the distribution of deformation from observation and simulation value of three different rupture models during 2010-2015.According to the parameters obtained by inversion of model 2,calculate the impact on crustal deformation in the surrounding area in next few decades after Wenchuan earthquake,the maximum cumulative postseismic deformation in 40 years during 2018-2058 up to 19 cm

NADPH Oxidase: a Target for the Modulation of the Excessive Oxidase Damage Induced by Overtraining in Rat Neutrophils

Objective: The purpose of this study is to demonstrate that NADPH oxidase mediating the ROS production is the major pathway for ROS generation in neutrophils during exercise. NADPH oxidase, as a target can modulate oxidative damage induced by overtraining, which can be value to the prevention of exercise-induced immunosuppression.Methods: Thirty male Wistar rats were randomly divided into three groups: a negative control group (C, n = 10), an overtraining group (E, n = 10) and an overtraining + DPI intervention group (D, n =10). Groups E and D were trained on a standard treadmill with progressive load for 11 weeks. After 36-40 h from the last training, eight rats were randomly selected from each group, and blood was sampled from the orbital vein. ELISAs were used to measure serum cytokine levels and lipid peroxidation in blood plasma. Flow cytometry with Annexin V / PI double staining was used to measure neutrophil apoptosis and necrosis. DNA damage in lymphocytes was tested using single cell gel electrophoresis (SCGE). The co-localization between gp91phox and p47phox of the NADPH-oxidase was detected using immunocytochemistry and confocal microscopy.Results: 1) Compared with group C, the concentrations of IL-1&#946;, IL-8, and TNF-&#945; were significantly increased and MCP-1, and CINC were significantly decreased in blood plasma from group E (P &#60; 0.01 and P &#60; 0.05, respectively). Concentrations of IL-1&#946; and MCP-1 were decreased (P &#60; 0.05), and IL-8 and TNF-&#945; were significantly increased (P &#60;0.05) in blood plasma from group D. MDA and MPO were elevated in plasma from groups E and D (P &#60; 0.01 and P &#60; 0.05, respectively). 2) Compared with group C, the percentage of neutrophils apoptosis were significantly elevated (P &#60; 0.01) in both groups E and D, and the percentage of cell death was raised in group E (P &#60; 0.05). No significant change was observed in group D. 3) Compared with group C, the number of comet cells, an indicator of DNA damage, was significantly increased (P &#60; 0.01), and the width and tail length of comet cells were notably increased in group E, while no significant increase was observed in group D. 4) The p47phox protein translocated to the cell membrane and co-localized with the gp91phox subunit of NADPH oxidase in neutrophils activated by overtraining.Conclusion: 1) Excessive exercise led to an increased secretion of inflammatory cytokines and chemokines in peripheral blood, and it may have induced tissue inflammation 2) Overtraining can activate the NADPH oxidase-mediated overproduction of ROS, leading to increased lipid peroxidation. 3) NADPHoxidase in neutrophils as a target, was responsible for ROS, oxidative damage to phagocytes and lymphocytes and changes to inflammatory cytokines and immune regulatory factors all affect cellular immune functions and may be causative factors for exercise-induced immunosuppression.</p

Methods to Improve the Accuracy and Robustness of Satellite-Derived Bathymetry through Processing of Optically Deep Waters

Selecting a representative optical deep-water area is crucial for accurate satellite-derived bathymetry (SDB) based on semi-theoretical and semi-empirical models. This study proposed a deep-water area selection method where potential areas were identified by integrating remote sensing imagery with existing global bathymetric data. Specifically, the effects of sun glint correction for deep-water areas on SDB estimation were investigated. The results indicated that the computed SDB had significant instabilities when different optical deep-water areas without sun glint correction were used for model training. In comparison, when sun glint correction was applied, the SDB results from different deep-water areas had greater consistency. We generated bathymetric maps for the Langhua Reef in the South China Sea and Buck Island near the U.S. Virgin Islands using Sentinel-2 multispectral images and 70% of the Ice, Cloud, and Land Elevation Satellite-2 (ICESat-2) bathymetry data. Additionally, 30% of the ICESat-2 bathymetry data and NOAA NGS Topo-bathy Lidar data served as the validation data to evaluate the qualities of the computed SDB, respectively. The results showed that the average quality of the SDB significantly improved with sun glint correction application by a magnitude of 0.60 m in terms of the root mean square error (RMSE) for two study areas. Moreover, an evaluation of the SDB data computed from different deep-water areas showed more consistent results, with RMSEs of approximately 0.4 and 1.4 m over the Langhua Reef and Buck Island, respectively. These values were consistently below 9% of the maximum depth. In addition, the effects of the optical image selection on SDB inversion were investigated, and the SDB calculated from the images over different time periods demonstrated similar results after applying sun glint correction. The results showed that this approach for optical deep-water area selection and correction could be used for improving the SDB, particularly in challenging scenarios, thereby enhancing the accuracy and robustness of SDB

Factor Analysis of Low-Frequency Repetitive Transcranial Magnetic Stimulation to the Temporoparietal Junction for Tinnitus

Objectives. We investigated factors that contribute to suppression of tinnitus after repetitive transcranial magnetic stimulation (rTMS). Methods. A total of 289 patients with tinnitus underwent active 1 Hz rTMS in the left temporoparietal region. A visual analog scale (VAS) was used to assess tinnitus loudness. All participants were interviewed regarding age, gender, tinnitus duration, laterality and pitch, audiometric parameters, sleep, and so forth. The resting motor thresholds (RMTs) were measured in all patients and 30 age- and gender-matched volunteers. Results. With respect to different factors that contribute to tinnitus suppression, we found improvement in the following domains: shorter duration, normal hearing (OR: 3.25, 95%CI: 2.01–5.27, p=0.001), and without sleep disturbance (OR: 2.51, 95%CI: 1.56–4.1, p=0.005) adjusted for age and gender. The patients with tinnitus lasting less than 1 year were more likely to show suppression of tinnitus (OR: 2.77, 95%CI: 1.48–5.19, p=0.002) compared to those with tinnitus lasting more than 5 years. Tinnitus patients had significantly lower RMTs compared with healthy volunteers. Conclusion. Active low-frequency rTMS results in a significant reduction in the loudness of tinnitus. Significant tinnitus suppression was shown in subjects with shorter tinnitus duration, with normal hearing, and without sleep disturbance

Transcriptomic Analysis Reveals an Altered Hcy Metabolism in the Stria Vascularis of the Pendred Syndrome Mouse Model

Purpose. Slc26a4-/- mice exhibit severer defects in the development of the cochlea and develop deafness, while the underlying mechanisms responsible for these effects remain unclear. Our study was to investigate the potential mechanism linking SLC26A4 deficiency to hearing loss. Materials and Methods. RNA sequencing was applied to analyze the differential gene expression of the stria vascularis (SV) from wildtype and Slc26a4-/- mice. GO and KEGG pathway analysis were performed. Quantitative RT-PCR was applied to validate the expression of candidate genes affected by Slc26a4. ELISA and immunofluorescence technique were used to detect the homocysteine (Hcy) level in serum, brain, and SV, respectively. Results. 183 upregulated genes and 63 downregulated genes were identified in the SV associated with Slc26a4 depletion. Transcriptomic profiling revealed that Slc26a4 deficiency significantly affected the expression of genes associated with cell adhesion, transmembrane transport, and the biogenesis of multicellular organisms. The SV from Slc26a4-/- mice exhibited a higher expression of Bhmt mRNAs, as well as altered homocysteine (Hcy) metabolism. Conclusions. The altered expression of Bhmt results in a dramatic change in multiple biochemical reactions and a disruption of nutrient homeostasis in the endolymph which may contribute to hearing loss of Slc26a4 knockout mouse