Persistence/recurrence-free rate of women after cervical conization.

<p>The patients were divided into three groups according to surgical margin and HPV status.</p

Correlation between demography and histopathology with recurrence.

<p>^<b>a</b><b>Pearson Chi-square test.</b></p><p>^<b>b</b><b>Mann-Whitney U test.</b></p><p>CKC, cold knife conization; LEEP, loop electrosurgical excision procedure; CIN, cervical intraepithelial neoplasia; HPV, human papilloma virus.</p

Characteristics of patients having LEEP/CKC (matched pairs).

<p>^<b>a</b><b>Mann-Whitney U test.</b></p><p>^<b>b</b><b>Pearson Chi-square test.</b></p><p>^<b>c</b><b>Monte Carlo exact test.</b></p><p>^<b>d</b><b>not the matched factor.</b></p><p>CKC, cold knife conization; LEEP, loop electrosurgical excision procedure; CIN, cervical intraepithelial neoplasia; HPV, human papilloma virus.</p

Allred score of immunohistochemical staining.

<p>^<b>a</b><b>Mann-Whitney U test.</b></p><p>^<b>b</b><b>Pearson Chi-square test.</b></p><p>CIN, cervical intraepithelial neoplasia; TPX2, xenopus kinesin-like protein 2; IMP3,insulin-like growth factor II messenger RNA (mRNA)-binding protein 3; PD-L1,programmed cell death-1 ligand-1.</p

Number of quadrants involvement and depth of gland involvement in CIN.

<p>^<b>a</b><b>Pearson Chi-square test.</b></p><p>^<b>b</b><b>Monte Carlo exact test.</b></p><p>^<b>c</b><b>Mann-Whitney U test.</b></p><p>CIN, cervical intraepithelial neoplasia; QI, quadrants involvement; GI, gland involvement; NA, not applicable.</p

Representive pictures of immunohistochemical staining of TPX2, IMP3, and PD-L1 in cervical tissues.

<p>Negative controls used non-immune isotype serum instead of primary antibodies. Arrows indicate the positively stained cells. Scale bars, 50 μm; original magnification, x200.</p

The levels of circulation Tc17 cells in representative controls, CIN patients and UCC patients.

<p>Upper right quadrants are the domains of Tc17 (CD8⁺ IL-17⁺) cells and the percentages of them were shown in each panel.</p

The frequency of Tc17 cells by immunohistochemical staining.

<p>(a) Compared to healthy controls (n = 30), significantly increased numbers of Tc17 cells were found in both tissues of UCC patients (<i>P</i> = 0.0007, n = 46) and CIN patients (<i>P</i> = 0.026, n = 28). Significant difference was also found between CIN and UCC tissues (<i>P</i> = 0.0086). (b) In tumor region, those UCC patients with lymph node metastases (n = 30) were detected significantly statistical higher Tc17 cells frequency than those patients without lymph node metastases (<i>P</i> = 0.035, n = 16). *<i>P</i><0.05, **<i>P</i><0.01, ****<i>P</i><0.001.</p

Expression of Tc17 cells in cervical tissues of the three groups.

<p>(A) Immunohistochemical double staining for Tc17 cells in the control group (a and d), CIN group (b and e) and UCC group (c and f). Representative sites with low (200×, upper panels) and high (400×, lower panels) magnification were shown. (B) IL-17-producing cells were stained red (in the cytoplasm) and CD8⁺ cells were stained black (in the membrane). The co-expression of CD8 and IL-17 confirmed that a proportion of Tc17 cells.</p

The correlations between Tc17 cells and microvessel density (MVD).

<p>(A) Linear regression analysis between the levels of Tc17 cells and MVD (Total: r = 0.987, <i>P</i><0.0001, n = 65; control: r = 0.814, <i>P</i><0.001, n = 18, CIN: r = 0.923, <i>P</i><0.001, n = 17; UCC: r = 0.938, <i>P</i><0.0001, n = 30). MVD from each sample was plotted against Tc17 cells level from the same person. (B) Representative immunohistochemical staining of MVD in cervical tissues of three groups. Representative sites with low (100×, upper panels) and high (400×, lower panels) magnification were shown.</p