Enhancement of Heat-Cured Cement Paste with Tannic Acid

The Improvement of Cement-Based Materials\u27 Performance by Natural Organic Compounds Can Greatly Promote the Green and Sustainable Development of the Construction Industry. However, Such Compounds Are Not Widely Used Yet Because of their Retarding Effect on Cement. in This Study, the Retardation Effect of Tannic Acid (TA, a Well-Known Retarding Compound) is overcome and the Enhancing Effect is Achieved by Adding Less Than 0.1% Content and Curing Samples in Thermal Regime. Then the Mechanism of TA Enhancing Heat-Cured Cement Pastes is Studied Systematically. Mechanical Properties Results Suggest that Addition of 0.025% TA Can Reduce the Compressive and Flexural Strengths of Cement Pastes by Up to 3.4% and 17.1% under Normal Curing Regime at 3 Days, But Enhance These Two Strengths by More Than 11.4% and 34.6% after Thermal Curing, Respectively. XRD Patterns and TGA Analysis Indicate that, under Thermal Curing Regime, 0.025% TA Can Improve the Hydration Degree of Cement Where the Bound Water Content is Increased by 21.4%. SEM Observations and MIP Results Show that TA Can Compact the Microstructure and the Porosity is Decreased by More Than 7.0%. Furthermore, FTIR Spectrums Prove that TA Can Bond with Hydration Products. Molecular Dynamics Simulation Demonstrates that TA Cross-Links with Calcium Silicate Hydrates (C–S–H) through Ionic and Hydrogen Bonds, Which Could Increase the Tensile Strength by 12.5% and the Ultimate Strain by 100%

DNA Replication Licensing Protein MCM10 Promotes Tumor Progression and Is a Novel Prognostic Biomarker and Potential Therapeutic Target in Breast Cancer

Breast cancer is one of the most common malignancies in women worldwide. In breast cancer, the cell proliferation rate is known to influence the cancer malignancy. Recent studies have shown that DNA replication initiation/licensing factors are involved in cancer cell proliferation as well as cancer cell migration and invasion. Licensing factors have also been reported as important prognostic markers in lung, prostrate, and bladder cancers. Here, we studied the role of MCM10, a novel licensing factor, in breast cancer progression. From the public database, NCBI, we investigated six independent breast cancer patient cohorts, totaling 1283 patients. We observed a significant association between high MCM10 mRNA expression with tumor grading and patients’ survival time. Most importantly, using breast cancer cohorts with available treatment information, we also demonstrated that a high level of MCM10 is associated with a better response to conventional treatment. Similarly, in in vitro studies, the expression level of MCM10 in breast cancer cell lines is significantly higher compared to paired normal breast epithelium cells. Knockdown of MCM10 expression in the cancer cell line showed significantly decreased tumorigenic properties such as cell proliferation, migration and anchorage independence. The MCF7 breast cancer cell line, after MCM10 expression knockdown, showed significantly decreased tumorigenic properties such as cell proliferation, migration, and anchorage independent growth. Mechanistically, MCM10 expression is observed to be regulated by an Estrogen Receptor (ER) signaling pathway, where its expression is suppressed by the inhibition of the ER or serum withdrawal. Our results suggest that MCM10 plays an important role in breast cancer progression and is a potential prognostic/predictive biomarker and therapeutic target for breast cancer patients

Cold-induced modulation and functional analyses of the DRE-binding transcription factor gene, GmDREB3, in soybean (Glycine max L.)

DREB (dehydration-responsive element-binding protein) transcription factors have important roles in the stress-related regulation network in plants. A DREB orthologue, GmDREB3, belonging to the A-5 subgroup of the DREB subfamily, was isolated from soybean using the RACE (rapid amplification of cDNA ends) method. Northern blot analysis showed that expression of GmDREB3 in soybean seedlings was induced following cold stress treatment for 0.5 h and was not detected after 3 h. However, it was not induced by drought and high salt stresses or by abscisic acid (ABA) treatment. This response was similar to those of members in the A-1 subgroup and different from those of other members in the A-5 subgroup, suggesting that the GmDREB3 gene was involved in an ABA-independent cold stress-responsive signal pathway. Furthermore, analysis of the GmDREB3 promoter elucidated its cold-induced modulation. A promoter fragment containing bases −1058 to −664 was involved in response to cold stress, and its effect was detected for 1 h after treatment, but a transcriptional repressor appeared to impair this response by binding to a cis-element in the region −1403 to −1058 at 24 h after the beginning of cold stress. Moreover, the GmDREB3 protein could specifically bind to the DRE element in vitro, and activated expression of downstream reporter genes in yeast cells. In addition, overexpression of GmDREB3 enhanced tolerance to cold, drought, and high salt stresses in transgenic Arabidopsis. Physiological analyses indicated that the fresh weight and osmolality of GmDREB3 transgenic Arabidopsis under cold stress were higher than those of wild-type controls. GmDREB3 transgenic tobacco accumulated higher levels of free proline under drought stress and retained higher leaf chlorophyll levels under high salt stress than wild-type tobacco. In addition, constitutive expression of GmDREB3 in transgenic Arabidopsis caused growth retardation, whereas its expression under control of the stress-inducible Rd29A promoter minimized negative effects on plant growth under normal growth conditions, indicating that a combination of the Rd29A promoter and GmDREB3 might be useful for improving tolerance to environmental stresses in crop plants

Association of Dopamine D2 Receptor Gene Polymorphisms with Reproduction Traits in Domestic Pigeons (Columba livia)

Dopamine inhibited prolactin secretion via dopamine D2 receptor (DRD2) at the pituitary level, but its effects on reproduction in pigeons are unclear. In this study, Single Nucleotide Polymorphisms (SNPs) in the exons of DRD2 gene were identified and analyzed by using DNA sequencing methods in 60 female domestic pigeons (Columba livia), and the association between DRD2 polymorphisms and reproduction traits was also analyzed. Sequencing results showed that 7 nucleotide mutations were detected in the exon 1, 4, and 6 regions of DRD2 gene. The analysis revealed three genotypes (AA, AB, and BB) in exon 4 and two genotypes (AA, AB) in exon 6, in which the AA genotype was consistently dominant, and the A allele showed a dominant advantage. The C4532T genotypes located in exon 6 of DRD2 gene were significantly (P<0.05) associated with reproductive traits of pigeon. Moreover, the individuals with AB genotype had significantly higher fertility rate and total hatching number within 500 days of age than those with AA genotype (P<0.05). These findings suggested that the DRD2 gene should be included in future genetic studies of pigeon reproduction and the SNP of C4532T might be a potential candidate genetic marker for Marker-aid breeding in pigeon

Enhancement of magnesium phosphate cement with sintered sludge ash

Magnesium phosphate cement (MPC) is a promising material known for its rapid hardening and high early-strength properties. However, its potential in engineering applications is hindered by its harden at an uncontrolled rate, considerable volumetric distortion and poor water resistance. To address the limitations, this study investigates the influence of sintered sludge ash (SSA) modification on the setting time, volume deformation, mechanical strength and water resistance of MPC composites. Experimental reveals that the setting time of MPC composites increase with higher SSA content, and the inclusion of SSA significantly mitigate volumetric deformation. Moreover, the mechanical strength exhibits improvement at all ages, and combine with water resistance analysis, 15% SSA content is the optimal level. Based on the XRD, TG, MIP and SEM, the enhanced properties of the MPC composites may be attributed to the reaction of SSA in the MPC system, leading to the resulting aluminum-containing gel phase acting as a binder along with guano to densify the microstructure

Microbial Community Composition and Activity in Saline Soils of Coastal Agro–Ecosystems

Soil salinity is a serious problem for agriculture in coastal regions. Nevertheless, the effects of soil salinity on microbial community composition and their metabolic activities are far from clear. To improve such understanding, we studied microbial diversity, community composition, and potential metabolic activity of agricultural soils covering non–, mild–, and severe–salinity. The results showed that salinity had no significant effect on bacterial richness; however, it was the major driver of a shift in bacterial community composition and it significantly reduced microbial activity. Abundant and diverse of microbial communities were detected in the severe–salinity soils with an enriched population of salt–tolerant species. Co–occurrence network analysis revealed stronger dependencies between species associated with severe salinity soils. Results of microcalorimetric technology indicated that, after glucose amendment, there was no significant difference in microbial potential activity among soils with the three salinity levels. Although the salt prolonged the lag time of microbial communities, the activated microorganisms had a higher growth rate. In conclusion, salinity shapes soil microbial community composition and reduces microbial activity. An addition of labile organic amendments can greatly alleviate salt restrictions on microbial activity, which provides new insight for enhancing microbial ecological functions in salt–affected soils

Strategies for the Efficient Estimation of Soil Moisture through Spectroscopy: Sensitive Wavelength Algorithm, Spectral Resampling and Signal-to-Noise Ratio Selection

It is found that the remote sensing parameters such as spectral range, spectral resolution and signal-to-noise ratio directly affect the estimation accuracy of soil moisture content. However, the lack of research on the relationship between the parameters and estimation accuracy restricts the prolongation of application. Therefore, this study took the demand for this application as the foothold for developing spectrometry. Firstly, a method based on sensitivity analysis of soil radiative transfer model-successive projection algorithm (SA-SPA) was proposed to select sensitive wavelengths. Then, the spectral resampling method was used to select the best spectral resolution in the corresponding sensitive wavelengths. Finally, the noise-free spectral data simulated by the soil radiative transfer model was added with Gaussian random noise to change the signal-to-noise ratio, so as to explore the influence of signal-to-noise ratio on the estimation accuracy. The research results show that the estimation accuracy obtained through the SA-SPA (RMSEP −1) is generally superior to that from full-spectrum data (RMSEP −1). At selected sensitive wavelengths, the best spectral resolution is 34 nm, and the applicable signal-to-noise ratio ranges from 150 to 350. This study provides technical support for the efficient estimation of soil moisture content and the development of spectrometry, which comprehensively considers the common influence of spectral range, spectral resolution and signal-to-noise ratio on the estimation accuracy of soil moisture content

Insights into the Interfacial Strengthening Mechanism of Waste Rubber/Cement Paste using Polyvinyl Alcohol: Experimental and Molecular Dynamics Study

Recycling of waste rubber into concrete is one of the most efficacious and environmentally-friendly methods to solve the so-called black pollution. However, the incorporation of waste rubber particles reduces the mechanical properties of concrete, resulting from the low stiffness of the rubber particles as well as the insufficient adhesion energy between waste rubber and hardened cement paste. To solve the latter issue, an innovative surface treatment method for hydrophilicity improvement of waste rubber using polyvinyl alcohol (PVA) is proposed in this paper. The interfacial strengthening mechanism of waste rubber/cement paste using PVA is systematically studied from the aspects of macro-scopic mechanical properties, micro-structure characteristics and molecular-scale modifications. The addition of 0.1% PVA solution can significantly enhance the compressive and flexural strength of rubber-cement composites by 7–14% and 20–38% at the age of 28 days, respectively. SEM observation and EDS results verify the bridging effect of PVA at the waste rubber/cement paste interface, which eliminates the gap caused by the hydrophobicity of rubber. The molecular dynamics simulation shows that the interfacial adhesion energy between rubber hydrocarbon (RH) and PVA is 52% higher than that between RH and calcium-silicate-hydrate (C–S–H), resulting in the accumulation of PVA molecules on rubber surface by van der Waals force and electrostatic interaction. The RH/PVA/C–S–H model has better mechanical properties than the RH/C–S–H model, verifying the bridging role of PVA at the molecular scale