Exclusive Enteral Nutrition versus Infliximab in Inducing Therapy of Pediatric Crohn’s Disease

Aim. To compare the effectiveness of exclusive enteral nutrition (EEN) and infliximab (IFX) therapy in pediatric Crohn’s disease (CD). Methods. In a prospective study of children initiating EEN or infliximab therapy for CD, we compared clinical outcomes using the pediatric Crohn’s disease activity index (PCDAI), growth improvement, endoscopic mucosal healing, and adverse effects. Data were measured at baseline and after 8 weeks of therapy. Results. We enrolled 26 children with CD; of whom, 13 were treated with infliximab, 13 with EEN. Clinical response (PCDAI) reduction ≥ 15 or final PCDAI ≤ 10 was achieved by 83.3% in the EEN group and 90.9% in the IFX group. Body mass index for age (BMIFA) z-scores were significantly increased in both groups (P<0.05). No significant differences were observed in PCDAI, height for age (HFA), or BMI recovery between two groups. Adverse effects were detected in 30.7% on infliximab and 0% on EEN. Mucosal healing was achieved in 71.4% cases in the EEN group versus 85.7% in the IFX group. Conclusion. EEN provided similar improvements as IFX in clinical symptoms, mucosal healing, and BMI. EEN therapy has less adverse effects when compared with IFX. This trial is registered with the Clinical Registration Number: ChiCTR-OON-17010834

Using a Deep Neural Network with Small Datasets to Predict the Initial Production of Tight Oil Horizontal Wells

Due to its abundant reserves, tight oil has emerged as a significant substitute for conventional petroleum resources. It has become one of the focal points of exploration and research, and a new hot spot in global unconventional oil and gas exploration and development. This has led to a significant increase in the demand for forecasting the production capacity of tight oil horizontal wells. The deep neural network (DNN), as a mature model, has demonstrated significant advantages in many fields. However, due to the confidentiality and uniqueness of oilfield data, acquiring large datasets has become a challenge. Traditional methods using small datasets for training DNN models result in low accuracy and overfitting issues, which hinders the development of neural networks in the petroleum industry. This study aims to predict the initial production capacity of tight oil horizontal wells by using a small dataset of 650 data points through a DNN model. The research results indicate that pre-trained and fine-tuned DNNs outperform shallow neural networks, supporting vector machines, and DNN trained with traditional methods in terms of better generalization performance. Their accuracy reached 91.3%, demonstrating that it is reasonable to use a small dataset with pre-trained and fine-tuned DNN models

A case of Metaplastic atrophic gastritis in immune Dysregulation, Polyendocrinopathy, Enteropathy, X-linked (IPEX) syndrome

Abstract Background Autoimmune metaplastic atrophic gastritis is a chronic progressive inflammatory condition. The clinical spectrum includes pernicious anemia, atrophic gastritis, antibodies to parietal cell antigens and intrinsic factor, achlorhydria, hypergastrinemia and carcinoma. It is rare in paediatric cohorts. Case presentation We present the case of a boy with metaplastic atrophic gastritis in whom immune dysregulation, polyendocrinopathy, enteropathy, X-linked(IPEX) syndrome was confirmed by FOXP3 gene mutation. The patient was referred to the hospital at the age of 3 years with recurrent emesis, diarrhoea and malnutrition. His elder brother died at 9 years of age from acute respiratory distress syndrome and renal tubular acidosis. The patient was allergic to cow milk formula and noodles. Oesophagegastroduodenoscopy revealed redness, erosion and edema throughout the stomach; whitish granules in the duodenal bulb; and edema in the second part of the duodenum. Biopsies showed extensive villous atrophy and goblet cell depletion in the duodenum. He was diagnosed with type-1 diabetes mellitus (T1DM) during the treatment of methylprednisolone. Serum antibodies against glutamic acid decarboxylase and pancreatic islets were detected. The patient’s FOXP3 gene was sequenced; this identified that the patient was hemizygous for a pathogenic variant [NM_014009.3:c.748_750del (p.Lys250del)]. Conclusion Metaplastic atrophic gastritis is rarely reported in patients with IPEX. Clinical gastroenterologists should be aware of IPEX syndrome when facing the complex syndromes of metaplastic atrophic gastritis and endocrinopathy

Sterigmatocystin induced cytotoxicity and disturbed lipid metabolism

Sterigmatocystin (STC), as a mycotoxin, widely exists the environment and food, and threatens the health of animals and human. Since STC has carcinogenicity and cancer is closely related to dysregulation in lipid metabolism, it is necessary to identify the effect of STC on lipid metabolism in vitro. In this research, the cytotoxicity of STC on HL-7702 cells were evaluated, analyzed by cell viability, reactive oxygen species (ROS) levels and relative genes transcription levels. Meanwhile, lipid changes were measured by oil red O staining. Further, HPLC-MS-based untargeted lipidomics were exploited to determine the alteration in endogenous lipid metabolites when exposed to STC. The results showed that the treatment of STC decreased cell viability, increased ROS levels, triggered abnormal cell cycle-related genes expression and induced apoptosis. Besides, STC exposure resulted in abnormal accumulation of lipid droplets. Lipidomics analysis further revealed that STC induced abnormal lipids metabolism, including lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), ceramides (Cer), cholesterol ester (ChE), fatty acid (FA), triglyceride (TG) and acyl carnitine (AcCa). Meanwhile, lipid species of LPC (16:0), LPC (16:1), LPC (18:0), LPC (18:1), LPC (18:2), LPE (16:0), and FA (20:4) are significantly decreased, but TG (16:0/16:1/16:1) and TG (18:1/18:1/18:2) increased dramatically. To sum up, these findings provided fresh insights to explore cytotoxic mechanism of STC

Short-Term Efficacy of Exclusive Enteral Nutrition in Pediatric Crohn’s Disease: Practice in China

Aims. The objective of this study was to compare the efficacy of exclusive enteral nutrition (EEN) and corticosteroids in inducing remission in pediatric Crohn’s disease (CD) and the effects of the treatment on growth improvements. Methods. Data was retrospectively collected for children and adolescents newly diagnosed with CD in a referral center. Patients who were followed up for more than 2 months with mild to moderate disease were included. Basic demographics, history, physical examination, the pediatric Crohn disease activity index (PCDAI), laboratory findings, endoscopic findings, and adverse effects were recorded. Remission was defined as PCDAI < 10 points. Results. Ten subjects received EEN and 18 patients received corticosteroids. The median follow-up in EEN group and steroid group was 9.2 weeks and 9.6 weeks, respectively. The remission rate in EEN group was significantly higher than that in steroid group (90.0% versus 50.0%, resp., P<0.05). Growth improvement, which was evaluated by changes in height for age z-score, was more apparent in EEN group than that in steroids group P<0.05. No adverse effects were observed in EEN group. Conclusions. In children with mild to moderate CD, EEN is more effective than corticosteroids in improving disease severity and growth deficiency, as well as providing less side effects

Niacin alters the ruminal microbial composition of cattle under high-concentrate condition

To understand the effects of niacin on the ruminal microbial ecology of cattle under high-concentrate diet condition, Illumina MiSeq sequencing technology was used. Three cattle with rumen cannula were used in a 3 × 3 Latin-square design trial. Three diets were fed to these cattle during 3 periods for 3 days, respectively: high-forage diet (HF; forage-to-concentrate ratio = 80:20), high-concentrate diet (HC; forage-to-concentrate ratio = 20:80), and HC supplemented with 800 mg/kg niacin (HCN). Ruminal pH was measured before feeding and every 2 h after initiating feeding. Ruminal fluid was sampled at the end of each period for microbial DNA extraction. Overall, our findings revealed that subacute ruminal acidosis (SARA) was induced and the α-diversity of ruminal bacterial community decreased in the cattle of HC group. Adding niacin in HC could relieve the symptoms of SARA in the cattle but the ruminal pH value and the Shannon index of ruminal bacterial community of HCN group were still lower than those of HF group. Whatever the diet was, the ruminal bacterial community of cattle was dominated by Bacteroidetes, Firmicutes and Proteobacteria. High-concentrate diet significantly increased the abundance of Prevotella, and decreased the abundance of Paraprevotella, Sporobacter, Ruminococcus and Treponema than HF. Compared with HC, HCN had a trend to decrease the percentage of Prevotella, and to increase the abundance of Succiniclasticum, Acetivibrio and Treponema. Increasing concentrate ratio could decrease ruminal pH value, and change the ruminal microbial composition. Adding niacin in HC could increase the ruminal pH value, alter the ruminal microbial composition

Assessing Hemorrhagic Shock Severity Using the Second Heart Sound Determined from Phonocardiogram: A Novel Approach

Introduction: Hemorrhagic shock (HS) is a severe medical emergency. Early diagnosis of HS is important for clinical treatment. In this paper, we report a flexible material-based heart sound monitoring device which can evaluate the degree of HS through a phonocardiogram (PCG) change. Methods: Progressive hemorrhage treatments (H1, H2, and H3 stage) were used in swine to build animal models. The PCG sensor was mounted on the chest of the swine. Routine monitoring was used at the same time. Results: This study showed that arterial blood pressure decreased significantly from the H1 phase, while second heart sound amplitude (S2A) and energy (S2E) decreased significantly from the H2 phase. Both S2A and S2E correlated well with BP (p &lt; 0.001). The heart rate, pulse pressure variation and serum hemoglobin level significantly changed in the H3 stage (p &lt; 0.05). Discussion: The change of second heart sound (S2) was at the H2 stage and was earlier than routine monitoring methods. Therefore, PCG change may be a new indicator for the early detection of HS severity

Niacin supplementation improves growth performance and nutrient utilisation in Chinese Jinjiang cattle

In the present study, the effect of niacin supplementation on the growth performance and nutrient utilisation in Chinese Jinjiang cattle was investigated. A total of 48 finishing male Jinjiang cattle aged 24–30 months, with body weights of 200 ± 15 kg, were randomly divided into four groups. The cattle were fed a finishing diet (concentrate to forage ratio of 80:20). The diets for the control, NA320, NA480 and NA640 groups were supplemented with 0, 320, 480 or 640 mg/kg of niacin, respectively. The body weight and feed consumption of the 48 cattle were recorded on days 1, 28, and 56 of the experiment. Assessment of feed digestibility was conducted from days 52 to 56 of the study. Blood samples were collected from each animal on day 56. From days 1 to 56 of the study, the results indicated a significant increase in the average daily weight gains (p < .05) and lower feed to gain ratios (p < .05) for the NA480 and NA640 groups than for the control group. Furthermore, supplementation with 640 mg/kg niacin increased the apparent digestibility of all nutrients (p < .05), whereas supplementation with 480 mg/kg niacin enhanced the apparent digestibility of crude protein (p < .05). Moreover, supplementation with 480 and 640 mg/kg niacin increased the content of serum nonesterified fatty acids (p < .05). Therefore, it was concluded that supplementation with 640 mg/kg niacin in a high-concentrate diet may be beneficial to growth and nutrient utilisation in Chinese Jinjiang cattle