Fatigue behavior of hybrid GFRP-concrete bridge decks under sagging moment

This paper presents a new cost-effective hybrid GFRP-Concrete deck system that the GFRP panel serves as both tensile reinforcement and stay-in-place form. In order to understand the fatigue behavior of such hybrid deck, fatigue test on a full-scale specimen under sagging moment was conducted, and a series of static tests were also carried out after certain repeated loading cycles. The fatigue test results indicated that such hybrid deck has a good fatigue performance even after 3.1 million repeated loading cycles. A three-dimensional finite element model of the hybrid deck was established based on experimental work. The results from finite element analyses are in good agreement with those from the tests. In addition, flexural fatigue analysis considering the reduction in flexural stiffness and modulus under cyclic loading was carried out. The predicted flexural strength agreed well with the analytical strength from finite element simulation, and the calculated fatigue failure cycle was consistent with the result based on related S-N curve and finite element analyses. However, the flexural fatigue analytical results tended to be conservative compared to the tested results in safety side. The presented overall investigation may provide reference for the design and construction of such hybrid deck system

Simultaneous Determination of 23 Mycotoxins in Broiler Tissues by Solid Phase Extraction UHPLC-Q/Orbitrap High Resolution Mass Spectrometry

Mycotoxins are a type of toxins harmful for not only animal but also human health. Cooccurrence of multi-mycotoxins could occur for food infected by several molds, producing multi-mycotoxins. It is necessary to develop corresponding determination methods, among which current mass spectrometry (MS) dominates. Currently, the accurate identification and quantitation of mycotoxins in complex matrices by MS with low resolution is still a challenge since false-positive results are typically obtained. Here, a method for the simultaneous determination of 23 mycotoxins in broiler tissues using ultra-high performance liquid chromatography-quadrupole/orbitrap HRMS was established. After the extraction by acetonitrile-water-formic acid (80:18:2, v/v/v), the purification by multifunctional purification solid phase extraction cartridges and the chromatographic separation on a C18 column, representative mycotoxins were determined by HRMS in full scan/data-dependent MS/MS acquisition mode. The quantitation was based on the external standard method. An MS/MS database of 23 mycotoxins was established to achieve qualitative screening and simultaneous quantification. Mycotoxins had a good linear relationship within a certain concentration range with correlation coefficients (r2) larger than 0.991 as well as the limit of quantitation of 1.80–300 μg/kg. The average recoveries at three different levels of low, medium and high fortification were 61–111% with relative standard deviations less than 13.5%. The method was fast, accurate, and suitable for the precise qualification of multiple mycotoxins in broiler tissues. 15 μg/kg zearalenone (ZEN) was detected in one liver sample among 30 samples from markets including chicken breast meat, liver, and gizzards. The result illustrated that the pollution of ZEN should not be neglected considering its harmful effect on the target organ of liver

Numerical parametric study on ultimate load and ductility of concrete encased equal-leg angle steel composite columns

Steel-concrete composite high bridge pier has been applied increasingly in China and around the world. Most applied steel type in the composite piers are H-shaped steel and steel pipe, while seldom research or practice is associated with angle steel. This paper conducted parametric study on the composite column with equal-leg angle steel and aimed to investigate the ultimate load and displacement ductility of the composite columns with different parameters. The parameters include the type of shear connector (stud and perfobond connectors), the type of structural steel (H-shaped steel and angle steel), steel-plate hooping ratio, shear-span ratio, and axial compression ratio. Finite element analysis was conducted for each specimen, which incorporated the concrete confinement effect, as well as the inelastic behavior of concrete, structural steel, and longitudinal and transverse steel bars. The equal-leg angle steel composite column was found to have slightly higher strength and displacement ductility than H-shaped steel composite column. The increase of steel-plate hooping result in larger strength and displacement ductility for the composite column, and the increase of shear-span ratio and axial compression ratio decrease the displacement ductility. Research results suggest stud and perfobond shear connectors should be applied as axial compression ratio being larger than 0.2 and 0.3, respectively. This paper provides reference for research and engineering practice of the concrete encased angle steel composite columns and bridge piers.Accepted Author ManuscriptSteel & Composite Structure

Assessment of Flexural Behavior of Pultruded GFRP Laminates for Bridge Deck Applications

In this study, flexural behaviors of the pultruded composite laminate were evaluated through experiments and theoretical analysis. Three-point flexural tests were performed for pultruded specimens. The typical failure mode for the longitudinal flexural specimens was local crush on the top surface accompanied with local cracks on the bottom surface at midspan. For the transverse tests, the specimens presented a failure pattern with local cracks initiated and propagated at both the top and bottom sides at the midspan. Theoretical analysis, based on micromechanics and macromechanics, was performed to predict flexural deformation and stress distribution of the pultruded laminate beam. Based on the continuum damage model proposed by the authors, this paper mainly investigates the flexural behavior and failure pattern of pultruded lamination. The theoretical and finite element results agreed well with the test results. The results can provide a reference for the design of the structural pultruded modular systems

Discovery of potent tubulin inhibitors targeting the colchicine binding site via structure-based lead optimization and antitumor evaluation

AbstractThe colchicine binding site of tubulin is a promising target for discovering novel antitumour agents. Previously, we identified 2-aryl-4-amide-quinoline derivatives displayed moderate tubulin polymerisation inhibitory activity and broad-spectrum in vitro antitumour activity. In this study, structure based rational design and systematic structural optimisation were performed to obtain analogues C1∼J2 bearing diverse substituents and scaffolds. Among them, analogue G13 bearing a hydroxymethyl group displayed good tubulin polymerisation inhibitory activity (IC50  =  13.5 μM) and potent antiproliferative activity (IC50 values: 0.65 μM∼0.90 μM). G13 potently inhibited the migration and invasion of MDA-MB-231 cells, and displayed potent antiangiogenic activity. It efficiently increased intracellular ROS level and decreased MMP in cancer cells, and obviously induced the fragmentation and disassembly of the microtubules network. More importantly, G13 exhibited good in vivo antitumour efficacy in MDA-MB-231 xenograft model (TGI  =  38.2%; i.p., 30 mg/kg)

Assessment of Flexural Behavior of Pultruded GFRP Laminates for Bridge Deck Applications

In this study, flexural behaviors of the pultruded composite laminate were evaluated through experiments and theoretical analysis. Three-point flexural tests were performed for pultruded specimens. The typical failure mode for the longitudinal flexural specimens was local crush on the top surface accompanied with local cracks on the bottom surface at midspan. For the transverse tests, the specimens presented a failure pattern with local cracks initiated and propagated at both the top and bottom sides at the midspan. Theoretical analysis, based on micromechanics and macromechanics, was performed to predict flexural deformation and stress distribution of the pultruded laminate beam. Based on the continuum damage model proposed by the authors, this paper mainly investigates the flexural behavior and failure pattern of pultruded lamination. The theoretical and finite element results agreed well with the test results. The results can provide a reference for the design of the structural pultruded modular systems.Steel & Composite Structure