In Vitro and in Vivo Effectiveness of Carvacrol, Thymol and Linalool against Leishmania infantum

Background: One of the most important causative agents of visceral leishmaniasis (VL) is Leishmania infantum, which is mainly spread by Phlebotomus and Lutzomyia sandflies in the Old and New World, respectively. Novel and effective drugs to manage this neglected vector-borne disease are urgently required. In this study, we evaluated the toxicity of carvacrol, thymol and linalool, three common essential oil constituents, on amastigotes and promastigotes of L. infantum. Methods: in vitro experiments were performed by 24 h MTT assay. Carvacrol, thymol and linalool at concentrations ranging from 1.3 to 10 µg/mL were tested on promastigotes of L. infantum. For in vivo test, two groups of hamsters (Mesocricetus auratus) received 100 mg/kg of body weight/day of carvacrol and thymol as intraperitoneal injection on day 7 post-infection, followed by a 48 h later injection. The third group was treated with the glucantime as standard drug (500 mg/kg) and the last group (control) just received normal saline. On the 16th day, the number of parasites and histopathological changes in liver and spleen were investigated. Results: 24 h MTT assay showed promising antileishmanial activity of thymol and carvacrol, with IC50 values of 7.2 (48 µM) and 9.8 µg/mL (65 µM), respectively. Linalool at all concentrations did not affect L. infantum promastigote viability. In vivo toxicity data of carvacrol and thymol showed that the former at 100 mg/kg was the safest and most effective treatment with little side effects on the liver. Conclusions: Overall, thymol and carvacrol are highly promising candidates for the development of effective and safe drugs in the fight against VL

A NEW SATELLITE IMAGERY STEREO PIPELINE DESIGNED FOR SCALABILITY, ROBUSTNESS AND PERFORMANCE

Abstract. This paper presents a new Multiview Stereo Pipeline (MVS), called CARS, dedicated to satellite imagery. This pipeline is intended for massive Digital Surface Model (DSM) production and has therefore been designed to maximize scalability robustness and performance. Those two properties have driven the design of the workflow as well as the choice of algorithms and parameter trends, making our pipeline unique with respect to existing solutions in literature. This paper intends to serve as a reference paper for the pipeline implementation, and therefore provides a detailed description of algorithms and workflow. It also demonstrates the pipeline robustness and stability in several use cases, and compares its accuracy with the state-of-the-art pipelines on a reference dataset. Document type: Articl

C/EBP beta-LIP induces cancer-type metabolic reprogramming by regulating the let-7/LIN28B circuit in mice

The transcription factors LAP1, LAP2 and LIP are derived from the Cebpb-mRNA through the use of alternative start codons. High LIP expression has been associated with human cancer and increased cancer incidence in mice. However, how LIP contributes to cellular transformation is poorly understood. Here we present that LIP induces aerobic glycolysis and mitochondrial respiration reminiscent of cancer metabolism. We show that LIP-induced metabolic programming is dependent on the RNA-binding protein LIN28B, a translational regulator of glycolytic and mitochondrial enzymes with known oncogenic function. LIP activates LIN28B through repression of the let-7 microRNA family that targets the Lin28b-mRNA. Transgenic mice overexpressing LIP have reduced levels of let-7 and increased LIN28B expression, which is associated with metabolic reprogramming as shown in primary bone marrow cells, and with hyperplasia in the skin. This study establishes LIP as an inducer of cancer-type metabolic reprogramming and as a regulator of the let-7/LIN28B regulatory circuit

Addressing Food Loss and Waste : A Global Problem with Local Solutions

The report focuses on the role that food loss and waste (FLW) could play in reducing the environmental footprint of food systems while attempting to meet the caloric and nutrient needs of a population expected to increase by 3 billion people in the next 30 years. The performance of the global food system over the last century has been extraordinary. From a global population of 1.6 billion people in 1900 to nearly 8 billion in 2020, the agri-food sector has risen to the challenge of providing global caloric sufficiency, mainly by increasing yields of a few principal staple crops. However, this path is no longer sustainable

Calcineurin Interacts with PERK and Dephosphorylates Calnexin to Relieve ER Stress in Mammals and Frogs

Background: The accumulation of misfolded proteins within the endoplasmic reticulum (ER) triggers a cellular process known as the Unfolded Protein Response (UPR). One of the earliest responses is the attenuation of protein translation. Little is known about the role that Ca 2+ mobilization plays in the early UPR. Work from our group has shown that cytosolic phosphorylation of calnexin (CLNX) controls Ca 2+ uptake into the ER via the sarco-endoplasmic reticulum Ca 2+-ATPase (SERCA) 2b. Methodology/Principal Findings: Here, we demonstrate that calcineurin (CN), a Ca 2+ dependent phosphatase, associates with the (PKR)-like ER kinase (PERK), and promotes PERK auto-phosphorylation. This association, in turn, increases the phosphorylation level of eukaryotic initiation factor-2 a (eIF2-a) and attenuates protein translation. Data supporting these conclusions were obtained from co-immunoprecipitations, pull-down assays, in-vitro kinase assays, siRNA treatments and [ 35 S]-methionine incorporation measurements. The interaction of CN with PERK was facilitated at elevated cytosolic Ca 2+ concentrations and involved the cytosolic domain of PERK. CN levels were rapidly increased by ER stressors, which could be blocked by siRNA treatments for CN-Aa in cultured astrocytes. Downregulation of CN blocked subsequent ER-stress-induced increases in phosphorylated elF2-a. CN knockdown in Xenopus oocytes predisposed them to induction of apoptosis. We also found that CLNX was dephosphorylated by CN when Ca 2+ increased. These data were obtained from [c 32 P]-CLN

Relationship between Adolescents' Nutritional and Physical Activity Behaviors with their Perceptions about Parents' Lifestyle Behaviors

Background & Aim: Obesity is increasing dramatically world-wide not only in adults, but also in children and adolescents. Nutritional and physical activity behaviors are related to the prevalence of obesity. Determining adolescents' behavioral patterns is important in preventing and adjusting this healthy problem. This study was carried out to assess lifestyle behaviors among girl adolescents and to determine the association between these behaviors and perceived parental lifestyle behaviors.Methods & Materials: In this cross-sectional study, 270 students were selected using multistage random sampling from girls' schools in the areas covered by Tehran University of Medical Sciences. Data were collected using a questionnaire. Descriptive and analytical tests were used to analyze the data.Results: Results showed that the mean age was 13.3 (10.7-16) years. Most of the adolescents' had normal limits of BMI. Only 9.2% of adolescents had optimal dietary behavior and 4.3% were physically active. There was not significant relationship between their nutritional behavior and their perceptions about parents' lifestyle. BMI and parents economic status was related to nutritional behaviors significantly. Physical activity was statistically related to BMI and mothers' education. Conclusion: This study showed that the adolescents' nutritional and physical activity behaviors were not healthy. Some factors are related to the adolescents' healthy behavior. Teaching healthy behaviors and defining healthy behavioral patterns for adolescent are necessary

Seroprevalence of Neospora caninum Infection in Rural and Industrial Cattle in Northern Iran

Background: Neospora caninum is an intracellular parasite which causes abortion in cattle worldwide.  The aim of this study was to determine the seroprevalence of N. caninum in cattle in Babol City, North of Iran."nMethods: Blood samples were collected from 237 cattle for determining the seroprevalence of N.  caninum.  A total of 237 serum samples were tested for anti-Neospora antibodies.  Serum samples were analyzed for antibodies against N. caninum antigen using a commercial N. caninum ELISA kit."nResults: Antibodies to N. caninum were found in 76 of 237 total cattle (32%), 40 of 155 industrial cat­tle (25. 8%) and 36 of 82 rural cattle sera (43. 9%) based on ELISA test results."nConclusion: This study is the first report of Neospora infection in this area.  Significant difference was observed regarding infection in industrial and rural cattle (P<0. 01)