USE OF CHROMAGAR ORIENTATION FOR PRESEMPTIVE IDENTIFICATION OF ENTEROCOCCI AND CHARACTERIZATION OF ANTIMICROBIAL RESISTANCE OF THE ISOLATES

This study was conducted to evaluate the use of CHROMagar Orientation for presumptive identification of enterococci from poultry, and to characterize the antimicrobial resistance of the isolates. Strains identification with conventional methods allowed confirmation of Enterococcus genus membership of all of the isolates on CHROMagar orientation (100%). Of the 141 enterococci isolates obtained from chicken, 81 (57.45%) were identified as Enterococcus faecalis and 60 (42.55%) as Enterococcus faecium. The antimicrobial susceptibility test presented high level of resistance to Tetracycline (89%) and Erythromycin (65%), low level of resistance to High Level of Streptomycin, Penicillin and Ciprofloxacin (13%, 10% and 9% respectively). Few of isolates were resistant to Chloramphenicol (2%). All the strains were susceptible to High Level of Gentamycin, Ampicillin, Vancomycin and Nitrofurantoin. The predominant phenotype of resistance pattern identified in both E.faecalis and E.faecium was (Erythromycin -Tetracycline)

ANTIBIOTIC RESISTANCE OF ENTEROCOCCI ISOLATED FROM POULTRY

This study was conducted to evaluate the antibiotic resistance of enterococci isolated from poultry in the wilaya of Tizi Ouzou. A total of 137 enterococci isolates from poultry were tested for antibiotic susceptibility by the diffusion method. Ten antibiotics from different families were tested. High percentages of resistance to Tetracycline and Erythromycin were observed (91.97% and 73.72% respectively), Low frequencies of antimicrobial resistance to Nitrofurantoin and Penicillin with a rates of 5.83% and 7.29% respectively. Resistance was recorded for high levels of Gentamycin and Streptomycin (8.75% and 22.62% respectively). However, all strains were sensitive to Vancomycin, Ciprofloxacin, Chloramphenicol and Ampicillin

Post-operative radiotherapy of conjunctival malignancies: A series of 24 cases

Objective: To assess the results of post-operative radiation therapy in the management of incompletely resected conjunctival malignancies. Methods: In this retrospective case series, we reviewed the clinical records of all cases of conjunctival tumors treated with post-operative radiotherapy in the radiation oncology department of Salah Azaïz Institute of Tunis, from January 1990 to December 2015. We focused on clinico-pathological characteristics, treatment modalities and patients’ outcome. Results: Twenty four patients were enrolled in our study: 19 men and 5 women. The mean age of our patients was 54 years (range: 20 to 84). The mean basal diameter of the tumor was 11 mm (range 6 to 20 mm). The mean tumor thickness was 4 mm (range 1 to 15 mm). The most frequent histological type was squamous cell carcinoma in 23 cases. One patient had a malignant conjunctival fibrohistiocytoma. Radiation therapy was post-operative for positive or narrow surgical margins in all cases. Eighteen patients were treated with kilovoltage radiation therapy (KVRT). The mean delivered dose to the tumor bed was 64 Gy (range: 60 to 70 Gy). Four patients were treated with an association of KVRT and Strontium 90 plaque brachytherapy. Two patients were treated only with Strontium 90 plaque brachytherapy (2 fractions of 17 Gy). After a median follow-up of 110 months, 19 patients were alive with no evidence of local recurrence in 17 patients. Two patients had a local recurrence and were referred to surgery. Two patients were lost to follow up. The 5-year relapse free survival rate was 90.9%. Radiation-induced side effects were conjunctivitis, cataract, eye watering and glaucoma. Conclusion: Post-operative radiation therapy allows good local control with acceptable toxicities in conjunctival malignancies. Management of these tumors needs a broad collaboration between ophthalmologists and radiation oncologists, to allow a conservative treatment with the lowest rates of local recurrence