3 research outputs found
Metabolite Profiling of Colvillea racemosa via UPLC-ESI-QTOF-MS Analysis in Correlation to the In Vitro Antioxidant and Cytotoxic Potential against A549 Non-Small Cell Lung Cancer Cell Line
In this study, flower and leaf extracts of Colvillea racemosa were considered a source of
bioactive compounds. In this context, the objective of the study focused on investigating the anticancer
potential as well as the phytochemical composition of both extracts. The extracts were analyzed by
UPLC-ESI-QTOF-MS, and the bioactivity was tested using in vitro antioxidant assays (FRAP, DPPH,
and ABTS) in addition to cytotoxic assays on non-small cell lung cancer cell line (A549). Our results
clearly indicated the potent radical scavenging capacity of both extracts. Importantly, the flower
extract exhibited a greater antioxidant capacity than the leaf extract. In terms of cytotoxic activity,
leaf and flower extracts significantly inhibited cell viability with IC50 values of 17.0 and 17.2 μg/mL,
respectively. The phytochemical characterization enabled the putative annotation of 42 metabolites,
such as saccharides, phenolic acids, flavonoids, amino acids, and fatty acids. Among them, the
flavonoid C-glycosides stand out due to their high relative abundance and previous reports on their
anticancer bioactivity. For a better understanding of the bioactive mechanisms, four flavonoids
(vitexin, kaempferol-3-O-rutinoside, luteolin, and isoorientin) were selected for molecular docking on
hallmark protein targets in lung cancer as represented by γ-PI3K, EGFR, and CDK2 through in-silico
studies. In these models, kaempferol-3-O-rutinoside and vitexin had the highest binding scores on
γ-PI3K and CDK2, followed by isoorientin, so they could be highly responsible for the bioactive
properties of C. racemosa extracts.Contract RYC2021-032119-I, founded by MCIN/AEI/10.13039/501100011033 and NextGenerationEU/PRTRSpanish Ministry of Science
and Innovation for the postdoctoral contract Juan de la Cierva-Formación (FJC2020-044298-I
Non-targeted metabolomics and chemometrics for saffron (Crocus sativus L.) authentication and adulteration detection in relation to its anticholinesterase activity
Saffron (Crocus sativus L.), is a spice of vulnerable medical importance. It is often adulterated to lower its quality and efficacy. The present study sought to detect the level of adulteration in five traded saffron collected from different localities using a high-performance liquid chromatography coupled with high resolution tandem mass spectrometry (UPLC-HR-MS/MS) technique. In total, 62 metabolites were identified in the five extracts namely from monoterpene aldehydes, flavonoid derivatives, apocarotenoids and carotenoids classes as well as chemical markers of adulteration. One common adulterant; Gardenia jasminoides Ellis was detected through the simultaneous determination of its specific constituents including geniposide, shanzhiside and major jasminosides. Also, one synthetic dye; auramine-O was detected in one marketed saffron product. The anticholinesterase activity of the five marketed saffron was also evaluated. Furthermore, saffron samples that contain high crocin levels possessed a promising anticholinesterase activity. It was detected for the first time that auramine-O dye is sometimes used as a potential adulterant of saffron. In spite of the grand researches on saffron, it still lacks an accurate evaluation of the commercial saffron products. The results of our study showed that high adulteration rate of trade saffron not only reduces saffron efficacy as a herbal remedy but may also simultaneously represent a health risk