Non-coding RNAs shuttled via exosomes reshape the hypoxic tumor microenvironment

Exosomes are small extracellular vesicles secreted by almost all the cells. Molecular cargos of exosomes can partially reflect the characteristics of originating cells. Exosome-mediated cell-to-cell interactions in the microenvironment are critical in cancer progression. Hypoxia, a key pro-cancerous feature of the tumor microenvironment, alters the releasing and contents of exosomes. A growing body of evidence shows that hypoxia induces more aggressive phenotypes in cancer. Of note, non-coding RNAs shuttled in hypoxic tumor-derived exosomes have been demonstrated as fundamental molecules in regulating cancer biology and remodeling tumor microenvironment. Furthermore, these hypoxic tumor-derived exosomal non-coding RNAs can be detected in the body fluids, serving as promising diagnostic and prognostic biomarkers. The current review discusses changes in cancer behaviors regulated by exosomes-secreted non-coding RNAs under hypoxic conditions.This work was supported by the grant from Samsung Research Funding Center of Samsung Electronics (project number: SPFC-MA1501-51). WW is a recipient of China Scholarship Council scholarship (CSC number: 201908260030)

Inhibition of MEK1/2 and GSK3 (2i system) affects blastocyst quality and early differentiation of porcine parthenotes

Inhibition of both MEK1/2 and glycogen synthase kinase-3 (GSK3; 2i system) facilitates the maintenance of naïve stemness for embryonic stem cells in various mammalian species. However, the effect of the inhibition of the 2i system on porcine early embryogenesis is unknown. We investigated the effect of the 2i system on early embryo development, expression of pluripotency-related genes, and epigenetic modifications. Inhibition of MEK1/2 (by PD0325901) and/or GSK3 (by CHIR99021) did not alter the developmental potential of porcine parthenogenetic embryos, but improved blastocyst quality, as judged by the blastocyst cell number, diameter, and reduction in the number of apoptotic cells. The expression levels of octamer-binding transcription factor 4 and SOX2, the primary transcription factors that maintain embryonic pluripotency, were significantly increased by 2i treatments. Epigenetic modification-related gene expression was altered upon 2i treatment. The collective results indicate that the 2i system in porcine embryos improved embryo developmental potential and blastocyst quality by regulating epigenetic modifications and pluripotency-related gene expression

Smart sensor systems for wearable electronic devices

Wearable human interaction devices are technologies with various applications for improving human comfort, convenience and security and for monitoring health conditions. Healthcare monitoring includes caring for the welfare of every person, which includes early diagnosis of diseases, real-time monitoring of the effects of treatment, therapy, and the general monitoring of the conditions of people's health. As a result, wearable electronic devices are receiving greater attention because of their facile interaction with the human body, such as monitoring heart rate, wrist pulse, motion, blood pressure, intraocular pressure, and other health-related conditions. In this paper, various smart sensors and wireless systems are reviewed, the current state of research related to such systems is reported, and their detection mechanisms are compared. Our focus was limited to wearable and attachable sensors. Section 1 presents the various smart sensors. In Section 2, we describe multiplexed sensors that can monitor several physiological signals simultaneously. Section 3 provides a discussion about short-range wireless systems including bluetooth, near field communication (NFC), and resonance antenna systems for wearable electronic devices

Truly form-factor–free industrially scalable system integration for electronic textile architectures with multifunctional fiber devices

Funding Information: This work was supported by the European Commission (H2020, 1D-NEON, grant agreement ID: 685758). J.M.K. and L.G.O. acknowledge the support from the U.K. Research and Innovation (EPSRC, EP/P027628/1). We thank Y. Bernstein and J. Faulkner for helping with grammar check. Funding Information: Acknowledgments Funding:ThisworkwassupportedbytheEuropeanCommission(H2020,1D-NEON,grant agreementID:685758).J.M.K.andL.G.O.acknowledgethesupportfromtheU.K.Researchand Innovation(EPSRC,EP/P027628/1).W ethankY .BernsteinandJ.Faulknerforhelpingwith grammarcheck.Authorcontributions:S.L.andJ.M.K.conceivedtheproject.S.L.,L.G.O.,P .B., R.Martins,andJ.M.K.supervisedtheproject.S.L.andH.L.developedF-PD.S.L.,Y .-W .L., G.-H.A., D.-W .S., J.I.S.,andS.C.developedF-SC.C.L.F ., A.S.,R.I.,P .B., andR.Martinsdevelopedfiber transistor.S.L.,H.L.,andS.C.developedF-LED.ThefiberdeviceswereevaluatedbyS.L.,H.W .C., D.-W .S., H.L.,S.J.,S.D.H.,S.Y .B., S.Z.,W .H.-C., Y .-H.S., X.-B.F ., T .H.L., J.-W .J., andY .K. The developmentofweavingprocesswasconductedbyS.L.,H.W .C., F .M.M., P .J., andV .G.C. Thelaser interconnectionwasdevelopedbyS.L.,H.W .C., K.U.,M.E.,andM.S.Thetextiledemonstrations werecharacterizedbyS.L.,H.W .C., D.-W .S., J.Y ., S.S.,U.E.,S.N.,A.C.,A.M.,R.Momentè,J.G.,N.D., S.M.,C.-H.K.,M.L.,A.N.,D.J.,M.C.,andY .C. ThismanuscriptwaswrittenbyS.L.andJ.M.K.and reviewed by H.W .C., D.-W .S., M.C.,L.G.O., P .B., E.F ., and G.A.J.A. All authors discussed the results andcommentedonthemanuscript.Competinginterests:Theauthorsdeclarethattheyhave nocompetinginterests.Dataandmaterialsavailability:Alldataneededtoevaluatethe conclusionsinthepaperarepresentinthepaperand/ortheSupplementaryMaterials. Publisher Copyright: Copyright © 2023 The Authors, some rights reserved.An integrated textile electronic system is reported here, enabling a truly free form factor system via textile manufacturing integration of fiber-based electronic components. Intelligent and smart systems require freedom of form factor, unrestricted design, and unlimited scale. Initial attempts to develop conductive fibers and textile electronics failed to achieve reliable integration and performance required for industrial-scale manufacturing of technical textiles by standard weaving technologies. Here, we present a textile electronic system with functional one-dimensional devices, including fiber photodetectors (as an input device), fiber supercapacitors (as an energy storage device), fiber field-effect transistors (as an electronic driving device), and fiber quantum dot light-emitting diodes (as an output device). As a proof of concept applicable to smart homes, a textile electronic system composed of multiple functional fiber components is demonstrated, enabling luminance modulation and letter indication depending on sunlight intensity.publishersversionpublishe

Recent progresses of near-eye display for AR/VR

Near-eye displays (NEDs) for augmented and virtual reality (AR/VR) are spotlighted because they have a possibility to provide much more immersive experiences never possible before. With the virtue of recent progress in sensors, optics, and computer science, several commercial products are already available, and the consumer market is expanding rapidly. However, there are several challenging issues for AR and VR NEDs to become closer to our lives. Here, we will explore these issues and important topics for AR and VR, and introduce some of the ideas to overcome them: diffractive optical elements (DOEs), retinal projection displays, and 3D display with focus cues. First, unlike VR with a simple optical system, AR that needs to merge an artificial image with an outer scene requires additional optics. The diffractive elements have the merit of being thin and transparent, suitable for the image combiner. Among them, holographic optical elements (HOEs) have great potential as they can record the desired volume grating from the simple lens to the complex wavefront using light interference. Second, in order to wear the NEDs for a long time, it must deal with the visual fatigue as well as the form factor. Retinal projection display can effectively prevent the vergence-accommodation conflict problem even with a simple optical design. In the retinal projection display, the light rays from the display are adjusted to converge into a small point using a lens. It ensures a wide depth range in which the images are clearly visible. Furthermore, it is possible to provide observers with accurate focus cues for the alleviation of visual fatigue via multi-layer displays and holographic displays. Recently, we conceived tomographic NED that can reproduce dense focal planes. We confirm that this system provides quasi-continuous focus cues, semi-original contrast, and considerable depth of field. The experimental results of our prototypes are explained. We also explain the recent activities of using deep learning in holographic NED system.N

Dynamically Manipulated Accommodation-invariant Displays

Computational accommodation-invariant (AI) display attempts to mitigate vergence-accommodation conflict (VAC) by showing a constant imagery no matter where the observer focuses on. However, due to the usage of an electrically focus-tunable lens, the contrast of imagery is degraded as point-spread functions of multiple foci are integrated. In this paper, we introduce the content-adaptive approach to improve the contrast at the depth of highly salient region in the image. The position of focal plane is dynamically determined considering the zone of comfort and the mean focal distance of salient region. The contrast enhancement compared to conventional accommodation-invariant display is shown through simulation results using USAF resolution target image. We demonstrate our proof-of-concept prototype and its optical feasibility is verified with experimental results.N

15 Focal Planes Head-Mounted Display using LED Array Backlight

Currently, commercial head-mounted displays suffer from limited accommodative states, which lead to vergence-accommodation conflict. In this work, we newly design the architecture of head-mounted display supporting 15 focal planes over wide depth of field (20cm-optical infinity) in real time to alleviate vergence-accommodation confict. Our system employs a low-resolution vertical scanning backlight, a display panel (e.g. liquid crystal panel), and focus-tunable lens. We demonstrate the compact prototype and verify its performance through experimental results.N

Volumetric Head-Mounted Display With Locally Adaptive Focal Blocks

A commercial head-mounted display (HMD) for virtual reality (VR) presents three-dimensional imagery with a fixed focal distance. The VR HMD with a fixed focus can cause visual discomfort to an observer. In this article, we propose a novel design of a compact VR HMD supporting near-correct focus cues over a wide depth of field (from 18 cm to optical infinity). The proposed HMD consists of a low-resolution binary backlight, a liquid crystal display panel, and focus-tunable lenses. In the proposed system, the backlight locally illuminates the display panel that is floated by the focus-tunable lens at a specific distance. The illumination moment and the focus-tunable lens' focal power are synchronized to generate focal blocks at the desired distances. The distance of each focal block is determined by depth information of three-dimensional imagery to provide near-correct focus cues. We evaluate the focus cue fidelity of the proposed system considering the fill factor and resolution of the backlight. Finally, we verify the display performance with experimental results.N

SSD-HDD-Hybrid Virtual Disk in Consolidated Environments

With the prevalence of multi-core processors and cloud computing, the server consolidation using virtualization has increasingly expanded its territory, and the degree of consolidation has also become higher. As a large number of virtual machines individually require their own disks, the storage capacity of a data center could be exceeded. To address this problem, copy-on-write storage systems allow virtual machines to initially share a template disk image. This paper proposes a hybrid copy-on-write storage system that combines solid-state disks and hard disk drives for consolidated environments. In order to take advantage of both devices, the proposed scheme places a read-only template disk image on a solid-state disk, while write operations are isolated to the hard disk drive. In this hybrid architecture, the disk I/O performance benefits from the fast read access of the solid-state disk, especially for random reads, precluding write operations from the degrading flash memory performance. We show that the hybrid virtual disk, in terms of performance and cost, is more effective than the pure copy-on-write disks for a highly consolidated system