Clínica e cirurgia de equinos

O presente relatório pretende descrever as atividades desenvolvidas no âmbito do estágio curricular do Mestrado Integrado em Medicina Veterinária da Universidade de Évora. Este relatório está separado em duas partes. Numa primeira parte apresenta-se a casuística acompanhada nos quatros meses de estágio nas diversas áreas da clínica geral de equinos, descrevendo-se alguns casos clínicos de forma mais específica. Na segunda parte é apresentada uma revisão bibliográfica sobre feridas contendo tecido de granulação, nas extremidades distais dos membros e o seu tratamento. Para terminar discutem-se três casos clínicos com diferente evolução do tecido de granulação. As feridas são das afeções mais comuns na clínica de equinos e, nesta espécie, umas das principais complicações é a formação excessiva de tecido de granulação. Desbridamento cirúrgico, corticosteroides, enxerto de pele e laser são alguns dos tratamentos a que se pode recorrer, embora algumas vezes nenhum deles seja eficaz; Equine clinic and surgery Abstract: The current report prentends to describe the activities developed in the ambit integrated internship of the master's degree in Veterinary Medicine of the University of Evora. This report is separated in two parts. In the first part it will be presented the casuistics followed in the four months of internship in the various areas of general equine practice, with some clinical cases being described more specifically. In the second part is presented a literature review about wounds with granulation tissue in the distal extremities of the limbs and their treatment. To finish, three clinical cases with diferent granulation tissue evolution are discussed. Wounds are the most common affections in the horse clinic, and in this specie, one of the main complications is the excessive formation of granulation tissue. Surgical debridement, corticosteroids, skin grafts and laser are some of the treatments that can be used, although sometimes none of them is effective

Evolution of Luminescence of Sr_{2–<i>y</i>–<i>z</i>}Ca_<i>z</i>Si(O_1–<i>x</i>N_<i>x</i>)₄:<i>y</i>Eu²⁺ with N^3–, Eu²⁺, and Ca²⁺ Substitutions

Sr_{2–<i>y</i>–<i>z</i>}Ca_<i>z</i>Si­(O_1–<i>x</i>N_<i>x</i>)₄­:<i>y</i>Eu²⁺ (SCSON:Eu²⁺) solid solutions were prepared by substituting N^3‑, Eu²⁺, and Ca²⁺ ions into Sr₂SiO₄ (SSO). These ions contributed differently to the evolution of luminescence of SCSON:Eu²⁺. SSON:Eu²⁺ (<i>z</i> = 0) has two activation centers: Eu­(I) and Eu­(II). The nitridation effects led to a dramatic change in the crystal field surrounding the Eu­(II) site but rarely affected the Eu­(I) site. Accordingly, SSON:Eu²⁺ exhibited broad excitation spectra from ultraviolet to visible wavelengths. In comparison with the Eu­(II) green emissions of SSO:Eu²⁺, the dominant peak wavelengths (DPWs) of the Eu­(II) emissions were at red emission regions (605–630 nm), depending on the amount of Eu²⁺ ions. The Ca²⁺ ions of SCSON:Eu²⁺ preferentially changed the emission wavelength assigned to Eu­(I) and affected the Eu­(II) emission intensity. In addition to the spectral properties, the chromaticity coordinates and a low thermal quenching behavior of SCSON:Eu²⁺ powders demonstrated that they can be a novel red phosphors for use in white light emitting diodes

Presentation1_Analyzing the Korean reference genome with meta-imputation increased the imputation accuracy and spectrum of rare variants in the Korean population.pptx

Genotype imputation is essential for enhancing the power of association-mapping and discovering rare and indels that are missed by most genotyping arrays. Imputation analysis can be more accurate with a population-specific reference panel or a multi-ethnic reference panel with numerous samples. The National Institute of Health, Republic of Korea, initiated the Korean Reference Genome (KRG) project to identify variants in whole-genome sequences of ∼20,000 Korean participants. In the pilot phase, we analyzed the data from 1,490 participants. The genetic characteristics and imputation performance of the KRG were compared with those of the 1,000 Genomes Project Phase 3, GenomeAsia 100K Project, ChinaMAP, NARD, and TOPMed reference panels. For comparison analysis, genotype panels were artificially generated using whole-genome sequencing data from combinations of four different ancestries (Korean, Japanese, Chinese, and European) and two population-specific optimized microarrays (Korea Biobank Array and UK Biobank Array). The KRG reference panel performed best for the Korean population (R2 = 0.78–0.84, percentage of well-imputed is 91.9% for allele frequency >5%), although the other reference panels comprised a larger number of samples with genetically different background. By comparing multiple reference panels and multi-ethnic genotype panels, optimal imputation was obtained using reference panels from genetically related populations and a population-optimized microarray. Indeed, the reference panels of KRG and TOPMed showed the best performance when applied to the genotype panels of KBA (R2 = 0.84) and UKB (R2 = 0.87), respectively. Using a meta-imputation approach to merge imputation results from different reference panels increased the imputation accuracy for rare variants (∼7%) and provided additional well-imputed variants (∼20%) with comparable imputation accuracy to that of the KRG. Our results demonstrate the importance of using a population-specific reference panel and meta-imputation to assess a substantial number of accurately imputed rare variants.</p

Location of LA out-pouching structures was divided into five categories: (A) near Bachmann bundle, (B) anterior, (C) inferoseptal, (D) inferior, and (E) lateral.

<p>Location of LA out-pouching structures was divided into five categories: (A) near Bachmann bundle, (B) anterior, (C) inferoseptal, (D) inferior, and (E) lateral.</p

Computational Simulation of the Activation Cycle of Gα Subunit in the G Protein Cycle Using an Elastic Network Model - Fig 3

<p><b>Mobility change of Gαs after the initial binding to β</b>_<b>2</b><b>AR either at the C-terminus (A) or the N-terminus (B).</b> The theoretical value at each residue was calculated by mean squared fluctuation between Gαβγ(GDP) and pre-R-Gαβγ(GDP) in percentile and is displayed according to the indicated color map. The residue in blue (red) is considered to become rigid (flexible). (C) Schematic of the proposed binding event between Gαs (gray) and β₂AR (blue). Activated β₂AR may initially be bound to the N terminus of Gα, leading to a relatively flexible C terminus, which increases the probability that it will find its proper binding position to the receptor. Eventually, this binding event is completed by the C terminal binding.</p

Effectiveness of automatic tube potential selection with tube current modulation in coronary CT angiography for obese patients: Comparison with a body mass index-based protocol using the propensity score matching method

<div><p>Background</p><p>Reduced image quality from increased X-ray scatter and image noise can be problematic when coronary computed tomography angiography (CCTA) imaging is performed in obese patients. The aim of this study was to compare the image quality and radiation dose obtained using automatic tube potential selection with tube current modulation (APSCM) with those obtained using a body mass index (BMI)-based protocol for CCTA in obese patients.</p><p>Methods</p><p>A total of 203 consecutive obese (BMI > 30 kg/m²) patients were retrospectively enrolled, of whom 96 underwent CCTA with APSCM and 107 underwent a BMI-based protocol. After applying the propensity score matching method, the clinical parameters, subjective and objective image quality, and radiation dose were compared between the APSCM group and the matched BMI-based group. These parameters were also compared among different tube potential subgroups.</p><p>Results</p><p>No significant differences were observed between the APSCM group and the BMI-based group with respect to image quality or radiation dose assessment (<i>p</i> > 0.05). Twenty patients (21%) examined with 140 kV in the APSCM group were exposed to significantly more radiation (<i>p</i> < 0.05) than patients in the BMI-based group or patients in the other APSCM kV subgroups; significant improvement in image quality was not observed in the 140 kV subgroup. Patients with a high BMI and a large effective diameter tended to be examined with 140 kV (<i>p</i> < 0.05).</p><p>Conclusion</p><p>The use of APSCM for CCTA in obese patients did not significantly reduce the radiation dose or improve image quality compared with those in the matched BMI-based group. Our data indicate that it is better to avoid using APSCM when 140 kV is automatically selected, due to increased radiation dose and lack of significant improvement in image quality.</p></div

Topological changes of the Gαs protein upon β₂AR binding.

<p>The Gαs structures in the Gαβγ(GDP) and R-Gαβγ(GDP) states are colored in gray and red, respectively. (A) Comparison of the structures of Gα with and without β₂AR. Compared to the GαsAH domain, the GαsRas domain shows marked displacement. (B) Topological change (displacement and torsional angle) of Gαs upon β₂AR binding. (C) The receptor-binding interface of Gαs. The distance between the α5 helix and α4-β6 decreases to 7 Å, which is within the interaction range. Red dotted lines represent new interactions between α5 helix and α4-β6 (D) Nucleotide-binding pocket (switch 2, P-loop, β6-α5 loop, and α5 helix). A significant change in the topology occurs near the nucleotide-binding pocket. The directions of movement of the α5 helix and P-loop are indicated by the yellow arrows.</p

Adsorption Behavior of Dyestuffs on Hollow Activated Carbon Fiber from Biomass

<div><p>This study focuses on the adsorption behavior of typical dyestuffs (methylene blue and reactive black 5) on hollow activated carbon fibers (ACFs) obtained from Kapok- and Hasuo-seed based biomass. It was found that the adsorption of dyestuffs on ACFs increased with increasing pH and temperature. In addition, the Hasuo-seed based ACFs showed higher adsorption capacities than the Kapok-seed based ACFs for dyestuffs. It was also determined from the adsorption energy distribution results that the ACFs are having energetically heterogeneous surfaces. The results clearly indicated that the prepared ACF in this study could efficiently remove dyes dissolved in water.</p></div

Simulation results of intermediate conformation states between the closed and open Gαs structures obtained using elastic network interpolation (ENI).

<p>(A) Possible states of Gαs. A large conformational change was observed around the GαsAH domain showing the swing motion with respect to the fixed GαsRas domain. (B) Root-mean-square deviation and cost function values of all intermediate conformational states with respect to the initial, closed Gαs structure. The smooth changes in both values represent the conformational changes and fluctuations in energy between the two reference structures of Gαs well. (C) Mobility changes during the transition. Intermediate conformations were more flexible than the two endpoint structures. Particularly, the mobility of the nucleotide-binding pocket region kept increasing during the transition. (D) Verification of the ENI pathway of the Gαs structure using the cumulative overlap values between the set of first 5% lowest normal modes and the directional vector to the target structure from each intermediate.</p

Patient characteristics and coronary CT angiography parameters.

<p>Patient characteristics and coronary CT angiography parameters.</p