The Between-Competition Running Demands of Elite Hurling Match-Play

The current study aimed to investigate the differences in running demands between the National Hurling League (NHL) and the Championship, and within playing positions. GPS (10 Hz, STATSports Apex GNSS) were used to analyse the running demands during 34 games (2017–2020 seasons) of the Championship and the NHL. The running demands (total-, relative-, high-speed- [\u3e17 km·h−1] and sprint [≥22 km·h−1] distance, number and length of sprints, and peak speed) were compared between competitions. Greater total- [ES = 0.32], relative- [ES = 0.26], and sprint-distance [ES = 0.41], and number of sprints [ES = 1.29] were completed in the Championship. The high-speed distance was similar between competitions. Half-backs and half-forwards covered greater total- (ES = 0.91 and 0.21, respectively), relative- (ES = 1.14 and 0.68, respectively), high-speed- (ES = 0.69 and 0.44, respectively), and sprint-distance (ES = 0.50 and 1.26, respectively), number of sprints (ES = 2.66 and 1.73, respectively), and peak speed (ES = 1.09 and 1.32, respectively) in the Championship. There was no difference (p \u3c 0.05) in the sprint distance covered between positions in the Championship. The results showed that the Championship is more physically demanding. The findings present key implications for the transition between competitions

The importance of a sport-specific stimulus for training agility

The purpose of this article is to review recent evidence to help guide the training of agility. agility skill usually involves reacting to a stimulus before performing a movement with a change of direction or velocity. research has shown that better performers can be distinguished from lower skilled athletes by the ability to quickly and accurately react to opponent's movements, but not to a generic stimulus such as a flashing light. therefore, training for agility should include a perceptual and decision-making component involving reacting to movements of others, and this may be accomplished with evasive drills or smallsided games. Copyright © National Strength and Conditioning Association

Distinct Biological Network Properties between the Targets of Natural Products and Disease Genes

We show that natural products target proteins with a high number of protein−protein functional interactions (high biological network connectivity) and that these protein targets have higher network connectivity than disease genes. This feature may facilitate disruption of essential biological pathways, resulting in competitor death. This result also suggests that additional sources of small molecules will be required to discover drugs targeting the root causes of human disease in the future.Chemistry and Chemical Biolog

Comparison of agility demands of small-sided games in elite Australian football

Purpose: To compare the agility demands of 4 small-sided games (SSGs) and evaluate the variability in demands for elite Australian Football (AF). Methods: Fourteen male elite Australian Football League (AFL) players (mean ± SD; 21.7 ± 3.1 y, 189.6 ± 9.0 cm, 88.7 ± 10.0 kg, 39.4 ± 57.1 games) completed 4 SSGs of 3 x 45-s bouts each with modified designs. Video notational analysis, GPS at 5 Hz, and triaxial accelerometer data expressed the external player loads within games. Three comparisons were made using a paired t test (P < .05), and magnitudes of differences were reported with effect size (ES) statistics. Results: Reduced area per player (increased density) produced a small increase in total agility maneuvers (SSG1, 7.2 ± 1.3; SSG2, 8.8 ± 4.1), while a large 2D player load was accumulated (P < .05, ES = 1.22). A reduction in players produced a moderate (ES = 0.60) total number of agility maneuvers (SSG 3, 11.3 ± 6.1; SSG 2, 8.3 ± 3.6); however, a greater variability was found. The implementation of a 2-handed-tag rule resulted in a somewhat trivial decline (P > .05, ES = 0.16) in agility events compared with normal AFL tackling rules (SSG 2, 8.3 ± 3.6; SSG 4, 7.8 ± 2.6). Conclusions: SSG characteristics can influence agility-training demand, which can vary considerably for individuals. Coaches should carefully consider SSG design to maximize the potential to develop agility for all players. © 2013 Human Kinetics, Inc

Prosaposin activates the androgen receptor and potentiates resistance to endocrine treatment in breast cancer.

INTRODUCTION: HOX genes play vital roles in growth and development, however, atypical redeployment of these genes is often associated with steroidal adaptability in endocrine cancers. We previously identified HOXC11 to be an indicator of poor response to hormonal therapy in breast cancer. In this study we aimed to elucidate genes regulated by HOXC11 in the endocrine resistant setting. METHODS: RNA-sequencing paired with transcription factor motif-mapping was utilised to identify putative HOXC11 target genes in endocrine resistant breast cancer. Validation and functional evaluation of the target gene, prosaposin (PSAP), was performed in a panel of endocrine sensitive and resistant breast cancer cell lines. The clinical significance of this finding was explored in clinical cohorts at both mRNA and protein level. RESULTS: PSAP was shown to be regulated by HOXC11 in both tamoxifen and aromatase inhibitor (AI) resistant cell lines. Transcript levels of HOXC11 and PSAP correlated strongly in samples of primary breast tumours (r = 0.7692, n = 51). PSAP has previously been reported to activate androgen receptor (AR) in prostate cancer cells. In a panel of breast cancer cell lines it was shown that endocrine resistant cells exhibit innately elevated levels of AR compared to their endocrine sensitive counterparts. Here, we demonstrate that stimulation with PSAP can drive AR recruitment to a hormone response element (HRE) in AI resistant breast cancer cells. Functionally, PSAP promotes cell migration and invasion only in AI resistant cells and not in their endocrine sensitive counterparts. In a cohort of breast cancer patients (n = 34), elevated serum levels of PSAP were found to associate significantly with poor response to endocrine treatment (p = 0.04). Meta-analysis of combined PSAP and AR mRNA are indicative of poor disease-free survival in endocrine treated breast cancer patients (hazard ratio (HR): 2.2, P = 0.0003, n = 661). CONCLUSION: The HOXC11 target gene, PSAP, is an AR activator which facilitates adaptation to a more invasive phenotype in vitro. These findings have particular relevance to the development of resistance to AI therapy which is an emerging clinical issue. PSAP is a secreted biomarker which has potential in identifying patients failing to exhibit sustained response to hormonal treatment

Caffeine, carbohydrate, and cooling use during prolonged simulated tennis

Purpose: To determine the effects of prolonged simulated tennis on performance and the ergogenic potential of caffeine, carbohydrates, and cooling. Methods: Twelve highly trained male tennis players (age 18.3 ± 3.0 y, height 178.8 ± 8.5 cm, body mass 73.95 ± 12.30 kg, mean ± SD) performed 4 simulated matches (2 h 40 min) against a ball machine on an indoor hard court. The counterbalanced experimental trials involved caffeine supplementation (3 mg/kg), carbohydrate supplementation (6% solution), precooling and intermittent cooling, and placebo control. Physiological markers (core temperature, heart rate, blood lactate, and blood glucose), subjective responses (ratings of perceived exertion and thermal sensation), stroke velocity and accuracy, serve kinematics, and tennis-specific perceptual skill quantified the efficacy of interventions. Results: Significant effects of time (P < .01) reflected increased physiological demand, reduced serve velocity and ground-stroke velocity and accuracy, and a slowing of the serve racket-arm acceleration phase. Caffeine increased serve velocity (165 ± 15 km/h) in the final set of the match (P = .014) compared with placebo (159 ± 15 km/h, P = .008) and carbohydrate (158 ± 13 km/h, P = .001) conditions. Carbohydrate and cooling conditions afforded physiological advantage (increased blood glucose, P < .01, and reduced preexercise thermal sensation, P < .01) but did not affect performance relative to the placebo condition. Conclusions: Prolonged simulated tennis induced significant decrements in tennis skills. Caffeine supplementation partly attenuated the effects of fatigue and increased serve velocity. In contrast, carbohydrate and cooling strategies had little ergogenic effect on tennis performance.C