18 research outputs found
THE INFLUENCE OF REGULAR TAl CHI EXERCISE ON NEUROMUSCULAR REACTION IN OLD PEOPLE
The purpose of this study was to investigate the impacts of regular Tai Chi (TC) exercise on neuromuscular reaction in old people. 21 old TC practitioners with regular practicing TC for more than 4 year and 22 sedentary non-practitioners were recruited to respectively form the TC group and sedentary control group. Electromyography (EiViG) was used to detect the neuromuscular reaction of the leg muscles to an unexpected ankle inversion perturbation. The latency of muscles, defined as the time from the moment of perturbation begins to the first EMG response (i.e. onset), was evaluated. The results indicated that regular TC practitioners activated their rectus femoris and anterior tibialis muscles to an unexpected perturbation significantly faster than their sedentary counterparts, which might be helpful for timely correction of postural disturbances
Wheat powdery mildew resistance: from gene identification to immunity deployment
Powdery mildew is one of the most devastating diseases on wheat and is caused by the obligate biotrophic phytopathogen Blumeria graminis f. sp. tritici (Bgt). Due to the complexity of the large genome of wheat and its close relatives, the identification of powdery mildew resistance genes had been hampered for a long time until recent progress in large-scale sequencing, genomics, and rapid gene isolation techniques. Here, we describe and summarize the current advances in wheat powdery mildew resistance, emphasizing the most recent discoveries about the identification of genes conferring powdery mildew resistance and the similarity, diversity and molecular function of those genes. Multilayered resistance to powdery mildew in wheat could be used for counteracting Bgt, including durable, broad spectrum but partial resistance, as well as race-specific and mostly complete resistance mediated by nucleotide-binding and leucine rich repeat domain (NLR) proteins. In addition to the above mentioned layers, manipulation of susceptibility (S) and negative regulator genes may represent another layer that can be used for durable and broad-spectrum resistance in wheat. We propose that it is promising to develop effective and durable strategies to combat powdery mildew in wheat by simultaneous deployment of multilayered immunity
Dynamics of Fecal Coliform Bacteria along Canada's Coast
The vast coastline provides Canada with a flourishing seafood industry
including bivalve shellfish production. To sustain a healthy bivalve molluscan
shellfish production, the Canadian Shellfish Sanitation Program was established
to monitor the health of shellfish harvesting habitats, and fecal coliform
bacteria data have been collected at nearly 15,000 marine sample sites across
six coastal provinces in Canada since 1979. We applied Functional Principal
Component Analysis and subsequent correlation analyses to find annual variation
patterns of bacteria levels at sites in each province. The overall magnitude
and the seasonality of fecal contamination were modelled by functional
principal component one and two, respectively. The amplitude was related to
human and warm-blooded animal activities; the seasonality was strongly
correlated with river discharge driven by precipitation and snow melt in
British Columbia, but such correlation in provinces along the Atlantic coast
could not be properly evaluated due to lack of data during winter.Comment: 30 pages, 7 figures, 0 tabl
MicroRNAs preferentially target the genes with high transcriptional regulation complexity
Over the past few years, microRNAs (miRNAs) have emerged as a new prominent
class of gene regulatory factors that negatively regulate expression of
approximately one-third of the genes in animal genomes at post-transcriptional
level. However, it is still unclear why some genes are regulated by miRNAs but
others are not, i.e. what principles govern miRNA regulation in animal genomes.
In this study, we systematically analyzed the relationship between
transcription factors (TFs) and miRNAs in gene regulation. We found that the
genes with more TF-binding sites have a higher probability of being targeted by
miRNAs and have more miRNA-binding sites on average. This observation reveals
that the genes with higher cis-regulation complexity are more coordinately
regulated by TFs at the transcriptional level and by miRNAs at the
post-transcriptional level. This is a potentially novel discovery of mechanism
for coordinated regulation of gene expression. Gene ontology analysis further
demonstrated that such coordinated regulation is more popular in the
developmental genes.Comment: supplementary data available at http://www.bri.nrc.ca/wan
Dietary exposure to 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) induces oxidative damage promoting cell apoptosis primarily via mitochondrial pathway in the hepatopancreas of carp, Cyprinus carpio
To investigate the mechanisms of BDE-47 on hepatotoxicity in fish, this study examined the effects of dietary exposure to BDE-47 (40 and 4000 ng/g) on carp for 42 days. The results showed that BDE-47 significantly increased carp’s condition factor and hepatosomatic index. Pathological results revealed unclear hepatic cord structure, hepatocytes swelling, cellular vacuolization, and inflammatory cell infiltration in the hepatopancreas of carp. Further investigation showed that ROS levels significantly increased on days 7, 14, and 42. Moreover, the activities of antioxidant enzymes SOD, GSH, CAT, and GST increased significantly from 1 to 7 days, and the transcription levels of antioxidant enzymes CAT, Cu-Zn SOD, Mn-SOD, GST, and GPX, and antioxidant pathway genes Keap1, Nrf2, and HO-1 changed significantly at multiple time-points during the 42 days. The results of apoptosis pathway genes showed that the mitochondrial pathway genes Bax, Casp3, and Casp9 were significantly upregulated and Bcl2 was significantly downregulated, while the transcription levels of FADD and PERK were significantly enhanced. These results indicate that BDE-47 induced oxidative damage in hepatopancreas, then it promoted cell apoptosis mainly through the mitochondrial pathway. This study provides a foundation for analyzing the mechanism of hepatotoxicity induced by BDE-47 on fish
Transcriptome Reveals the Effects of Early Weaning on Lipid Metabolism and Liver Health of Yangtze Sturgeon (Acipenser dabryanus)
The Yangtze sturgeon (Acipenser dabryanus) has recently been declared extinct in the wild, and artificial breeding is the only means to protect its germplasm resources, but it has difficulty in weaning (from live prey to artificial food). In this study, we first performed a histological observation, enzyme-activity determination, and transcriptome sequencing on the livers of juvenile Yangtze sturgeons, and we then cloned five critical genes of lipid metabolism according to the transcriptome-sequencing results. We designed a weaning experiment to analyze their expression levels during weaning. The results showed that the density of hepatocytes and the transaminase activity of the juveniles failed to wean. The differentially expressed genes were enriched significantly in the pathways involving steroid synthesis, amino acid metabolism, and pancreatic secretion. It was found that the mRNA level of the fatty acid-synthesis gene decreased, and the mRNA level of the lipolysis gene increased significantly during weaning. The results of this research indicated that weaning could affect the liver health of Yangtze sturgeon, and it could affect the liver lipid metabolism by inhibiting fatty acid synthesis and promoting lipolysis. This study enhances our understanding of the impact of weaning on the lipid metabolism in fish