Additional file 1 of Anaplastic lymphoma kinase overexpression enhances aggressive phenotypic characteristics of endometrial carcinoma

Additional file 1: Supplementary Figure S1. ALK expression in Em Ca cells. (A) ALK mRNA expression in 11 Em Ca cell lines. Note the strong mRNA signals in Hec59 and Hec251 cells and the weak signals in Ishikawa and Hec88 cells. (B) CCLE data analysis for ALK mRNA expression in 28 Em Ca cell lines, demonstrating the high ALK mRNA expression in Hec59 cells and the low level in Hec251 cells. (C) Western blot analysis for the indicated proteins in total lysates from Hec59 and Hec251 cells. Note the full length ALK protein expression (220 kDa) in Hec59 but not Hec251 cells. D) Mutation analysis of exons 20, 23, 24, and 25 of the ALK gene in Hec59 cells, demonstrating a lack of mutations in the four exons. (E) H6-ALK#47 cells are stained with anti-ALK antibody. Note the cytoplasmic ALK staining (indicated by arrows). Original magnification, x200

Additional file 3 of Anaplastic lymphoma kinase overexpression enhances aggressive phenotypic characteristics of endometrial carcinoma

Additional file 3: Supplementary Figure S3. Original images of western blot analysis for the indicated proteins in total lysates from H6-ALK (A), H59-shALK (B), and mock cells

Additional file 2 of Anaplastic lymphoma kinase overexpression enhances aggressive phenotypic characteristics of endometrial carcinoma

Additional file 2: Supplementary Figure S2. Original images of western blot analysis for the indicated proteins in total lysates from H6-ALK (A), H59-shALK (B), and mock cells

Additional file 5 of Anaplastic lymphoma kinase overexpression enhances aggressive phenotypic characteristics of endometrial carcinoma

Additional file 5: Supplementary Figure S5. TCGA data analysis for associations between ALK status and prognosis in Em Ca. OS (left) and PFS (right) relative to ALK mRNA (A) and the gene mutation status (B). n, number of cases. Statistical analyses were performed using the log rank test

Additional file 4 of Anaplastic lymphoma kinase overexpression enhances aggressive phenotypic characteristics of endometrial carcinoma

Additional file 4: Supplementary Figure S4. Original images of western blot analysis for the indicated proteins in total lysates from H6-ALK (A), H59-shALK (B), and mock cells. The predictive sizes are indicated by arrows. N-cad, N-cadherin

Additional file 6 of Anaplastic lymphoma kinase overexpression enhances aggressive phenotypic characteristics of endometrial carcinoma

Additional file 6: Supplementary Table S1. Relationship between ALK expression and clinicopathological factors in endometrial carcinoma

Additional file 7 of Anaplastic lymphoma kinase overexpression enhances aggressive phenotypic characteristics of endometrial carcinoma

Additional file 7: Supplementary Table S2. ALK mRNA expression and mutations in endometrial carcinoma cell lines

Additional file 9 of PTEN overexpression and nuclear β-catenin stabilization promote morular differentiation through induction of epithelial–mesenchymal transition and cancer stem cell-like properties in endometrial carcinoma

Additional file 8: Fig. S5. A Ishikawa cells were transfected with EBP50 reporter constructs, together with estrogen receptor α (ERα). Relative activity was determined based on arbitrary light units of luciferase activity normalized to pRL-TK activity. The activities of the reporter plus the effector relative to that of the reporter plus empty vector are shown as means ± SDs. B Ishikawa cells were transfected with Top reporter constructs, together with EBP50 and β-cateni

Additional file 7 of PTEN overexpression and nuclear β-catenin stabilization promote morular differentiation through induction of epithelial–mesenchymal transition and cancer stem cell-like properties in endometrial carcinoma

Additional file 6: Table S3. Relationship between PTEN expression and morular fomation and histological garde of endometrial carcinoma

Additional file 3 of PTEN overexpression and nuclear β-catenin stabilization promote morular differentiation through induction of epithelial–mesenchymal transition and cancer stem cell-like properties in endometrial carcinoma

Additional file 2: Fig. S1. PTEN expression and mutation in Hec6 cells. Upper: western blot analysis for PTEN proteins in total lysates from six Em Ca cell lines. Lower: sequencing analysis for exon 8 in the PTEN gene in Hec6 cell