Effects of different fertilization conditions and different geographical locations on the diversity and composition of the rhizosphere microbiota of Qingke (Hordeum vulgare L.) plants in different growth stages

IntroductionThe excessive use of chemical fertilizer causes increasing environmental and food security crisis. Organic fertilizer improves physical and biological activities of soil. Rhizosphere microbiota, which consist of highly diverse microorganisms, play an important role in soil quality. However, there is limited information about the effects of different fertilization conditions on the growth of Qingke plants and composition of the rhizosphere microbiota of the plants.MethodsIn this study, we characterized the rhizosphere microbiota of Qingke plants grown in three main Qingke-producing areas (Tibet, Qinghai, and Gansu). In each of the three areas, seven different fertilization conditions (m1–m7, m1: Unfertilized; m2: Farmer Practice; m3: 75% Farmer Practice; m4: 75% Farmer Practice +25% Organic manure; m5: 50% Farmer Practice; m6: 50% Farmer Practice +50% Organic manure; m7: 100% Organic manure) were applied. The growth and yields of the Qingke plants were also compared under the seven fertilization conditions.ResultsThere were significant differences in alpha diversity indices among the three areas. In each area, differences in fertilization conditions and differences in the growth stages of Qingke plants resulted in differences in the beta diversity of the rhizosphere microbiota. Meanwhile, in each area, fertilization conditions, soil depths, and the growth stages of Qingke plants significantly affected the relative abundance of the top 10 phyla and the top 20 bacterial genera. For most of microbial pairs established through network analysis, the significance of their correlations in each of the microbial co-occurrence networks of the three experimental sites was different. Moreover, in each of the three networks, there were significant differences in relative abundance and genera among most nodes (i.e., the genera Pseudonocardia, Skermanella, Pseudonocardia, Skermanella, Aridibacter, and Illumatobacter). The soil chemical properties (i.e., TN, TP, SOM, AN, AK, CEC, Ca, and K) were positively or negatively correlated with the relative abundance of the top 30 genera derived from the three main Qingke-producing areas (p < 0.05). Fertilization conditions markedly influenced the height of a Qingke plant, the number of spikes in a Qingke plant, the number of kernels in a spike, and the fresh weight of a Qingke plant. Considering the yield, the most effective fertilization conditions for Qingke is combining application 50% chemical fertilizer and 50% organic manure.ConclusionThe results of the present study can provide theoretical basis for practice of reducing the use of chemical fertilizer in agriculture

A Nonlinear Data-Driven Towed Array Shape Estimation Method Using Passive Underwater Acoustic Data

Large-aperture towed linear hydrophone array has been widely used for beamforming-based signal enhancement in passive sonar systems; however, its performance can drastically decrease due to the array distortion caused by rapid tactical maneuvers of the towed platform, oceanic currents, hydrodynamic effects, etc. In this paper, an enhanced data-driven shape array estimation scheme is provided in the passive underwater acoustic data, and a novel nonlinear outlier-robust particle filter (ORPF) method is proposed to acquire enhanced estimates of time delays in the presence of distorted hydrophone array. A conventional beamforming technique based on a hypothetical array is first used, and the detection of the narrow-band components is sequentially carried out so that the corresponding amplitudes and phases at these narrow-band components can be acquired. We convert the towed array estimation problem into a nonlinear discrete-time filtering problem with the joint estimates of amplitudes and time-delay differences, and then propose the ORPF method to acquire enhanced estimates of the time delays by exploiting the underlying properties of slowly changing time-delay differences across sensors. The proposed scheme fully exploits directional radiated noise targets as sources of opportunity for online array shape estimation, and thus it requires neither the number nor direction of sources to be known in advance. Both simulations and real experimental data show the effectiveness of the proposed method

Study on stress and deformation characteristics of existing-new two-stage cantilever retaining wall.

A two-stage cantilever retaining wall is composed of two single-stage cantilever retaining walls, which are stacked up and down. The structure not only has the advantages of a single-stage retaining wall, but also compensates for the shortcomings of the height limit of the single-stage retaining wall; therefore, it has been gradually applied in projects. Based on the actual project of Zhongwei-Lanzhou Passenger Dedicated Line into Lanzhou Hub, this paper studies the influence of the construction of new cantilever retaining wall and the filling of subgrade on the deformation and earth pressure of the new cantilever wall and the existing cantilever wall by means of field test and numerical simulation. The results show that with an increase in the filling height after the new cantilever wall (upper wall), the horizontal displacement of the top of the upper and lower walls increased nonlinearly. The displacement direction of the upper wall was the filling direction, and that of the lower wall was the deviation from the filling direction. The higher the filling height, the greater is the displacement. With an increase in the filling height, the earth pressure behind the upper wall increases gradually along the wall height and decreases slightly to the bottom of the wall, which is approximately a linear distribution. The earth pressure behind the existing cantilever wall first increases along the wall height and gradually decreases after reaching a certain depth, but the earth pressure of the lower wall does not increase significantly with an increase in the filling height behind the upper wall. The slope failure mode is the overall sliding failure of the retaining wall together with the fill soil. The sliding surface passed through the lower edge of the lower wall heel and was similar to an arc shape. The stability of the two-stage cantilever retaining wall was better than that of a single-stage retaining wall. Finally, a calculation method for the overall stability and earth pressure of the existing two-stage cantilever retaining wall was proposed

Aggressive natural killer cell leukemia or extranodal NK/T cell lymphoma? a case with nasal involvement

Abstract Background Aggressive natural killer cell leukemia/lymphoma (ANKL) is a rare and highly aggressive NK cell neoplasm with a short clinical course and poor prognosis and is often misdiagnosed and confused with NK/T cell lymphoma (NKTL), which has a very different prognosis. Here, we present a case with nasal and bone marrow involvement, provide a literature review and make a differential diagnosis. Case presentation A 41-year-old male presented nasal congestion pharyngalgia, palatal perforation, high fever and multiorgan dysfunction. Our diagnosis primarily relied on clinical features, the morphology and immunophenotype of the neoplastic cells and imaging studies. Characteristic large granular lymphocytes with azurophilic granules were visible in the bone marrow smears. In addition, the neoplastic cells expressed a typical immunophenotype, and the T cell receptor γ (TCR-γ) gene rearrangement analysis and presence of Epstein-Barr virus (EBV) were negative. The patient’s symptoms and signs were temporarily relieved after chemotherapy treatment, but after a short time, he underwent a rapid clinical decline and died 8 weeks later after admission due to multiorgan function failure. Conclusion Our case demonstrates that to avoid a misdiagnosis, bone marrow analyses and other examinations should be performed early when a patient initially presents nasal lesions and other systemic symptoms. To the best of our knowledge, this may be the first reported case of ANKL with sternal tenderness

Three-dimensional model of two-stage cantilever retaining wall.

Three-dimensional model of two-stage cantilever retaining wall.</p

Horizontal displacement curve of wall body, (a)The upper wall, (b)The lower wall.

Horizontal displacement curve of wall body, (a)The upper wall, (b)The lower wall.</p

The physical and mechanical parameters of soil layer.

The physical and mechanical parameters of soil layer.</p