Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients

Historically, Japanese Encephalitis virus (JEV) genotype III (GIII) has been responsible for human diseases. In recent years, JEV genotype I (GI) has been isolated from mosquitoes collected in numerous countries, but has not been isolated from patients with encephalitis. In this study, we report recovery of JEV GI live virus and identification of JEV GI RNA from cerebrospinal fluid (CSF) of encephalitis patients in JE endemic areas of China. Whole-genome sequencing and molecular phylogenetic analysis of the JEV isolate from the CSF samples was performed. The isolate in this study is highly similar to other JEV GI strains which isolated from mosquitoes at both the nucleotide and deduced amino acid levels. Phylogenetic analysis based on the genomic sequence showed that the isolate belongs to JEV GI, which is consistent with the phylogenetic analysis based on the pre-membrane (PrM) and Glycoprotein genes. As a conclusion, this is the first time to isolate JEV GI strain from CSF samples of encephalitis patients, so continuous survey and evaluate the infectivity and pathogenecity of JEV GI strains are necessary, especially for the JEV GI strains from encephalitis patients. With respect to the latter, because all current JEV vaccines (live and inactivated are derived from JEV GIII strains, future studies should be aimed at investigating and monitoring cross-protection of the human JEV GI isolates against widely used JEV vaccines

Effect of Top Tension on Vortex-Induced Vibration of Deep-Sea Risers

With the increase of water depth, the design and use of the top-tensioned risers (TTR) are facing more and more challenges. This research presents the effect of top tension on dynamic behavior of deep-sea risers by means of numerical simulations and experiments. First, the governing equation of vortex-induced vibration (VIV) of TTR based on Euler-Bernoulli theory and Van der Pol wake-oscillator model was established, and the effect of top tension on natural vibration of TTR was discussed. Then, the dynamic response of TTR in shear current was calculated numerically by finite difference method. The displacement, bending stress and vibration frequency of TTR with the variation of top tension were investigated. Finally, a VIV experiment of a 5 m long flexible top-tensioned model was carried out at the towing tank of Tianjin University. The results show that the vibration displacement of TTR increases and the bending stress decreases as the top tension increases. The dominant frequency of VIV of TTR is controlled by the current velocity and is barely influenced by the top tension. With the increase of top tension, the natural frequency of TTR increases, the lower order modes are excited in the same current

Numerical and Experimental Research on the Effect of Platform Heave Motion on Vortex-Induced Vibration of Deep Sea Top-Tensioned Riser

The prediction and control of vortex-induced vibration (VIV) is one of the key problems for riser design. The effect of platform heave motion on VIV of deep sea top-tensioned riser (TTR) is presented by means of numerical simulation and experiment in this research. First, the heave motion was modeled as a parametric excitation, and the governing equation of VIV of riser considering the parametric excitation was established. Then, the dynamic response of TTR was calculated numerically by the finite difference method based on the Van der Pol wake-oscillator model. Finally, a validation experiment was carried out at the towing tank of Tianjin university. The results show that the VIV response at the bottom of riser is significantly increased due to the platform heave motion, especially in the situation of low current velocity. The larger amplitude and the higher frequency of the platform heave motion with the greater influence are generated on VIV of TTR. In particular, the value of 0.5 times, 1 time, or other multiples of the platform heave frequency will be included in the vibration frequency component of TTR when the platform heave amplitude is large and the frequency is high

An Analytical Model of Floating Offshore Wind Turbine Blades Considering Bending-Torsion Coupling Effect

In this paper, an analytical model is proposed to describe the nonlinear vibration of blades on floating offshore wind turbine (FOWT). The bending-torsion coupling equations are derived based on Hamilton’s principle. Comparing with the classical Newtonian method, this approach is more mathematically rigorous and systematic. The flapwise and edgewise deformation, the torsion as well as axial extension of the blades are all included in the model. A set of partial differential equations governing the coupled nonlinear vibration is established, and the results are compared with the multi-body model. Some details about the solution of equations are discussed. The eigen values of a rotating blade is also calculated. The structural model proposed in this paper can be widely used in the future study. For example, it can be coupled with an aerodynamic model to study the aeroelastic properties of the wind turbine blades. The effect of platform motion on blade dynamic response can also be obtained based on this analytical model

Rotational photonic spin Hall effect

Multidimensional manipulation of photonic spin Hall effect (PSHE) has attracted considerable interest due to its potential in a wide variety of spin-based applications. Plenty of research efforts have been devoted to transverse or longitudinal spin-dependent splitting; however, the splitting pattern that can self-rotate in a three-dimensional (3-D) space appears to be missing in literature. In this paper, we introduce a novel 3-D rotational PSHE, which can be realized and tuned using well-designed Pancharatnam–Berry phase metasurfaces. To demonstrate this phenomenon, we first show that when a single dielectric metasurface is used, the lobe-structured spin-splitting patterns on the transverse planes rotate and evolve along the propagation path. Then, we present that under two cascaded metasurfaces, the rotation angle of the splitting patterns are tunable by adjusting the relative rotation angle between the two metasurfaces. Finally, we manifest that the lobe number of the two spin-dependent splitting patterns can be independently controlled once we introduce a dynamic phase, which produces an asymmetrical rotational PSHE. The demonstrated phenomena can be used to achieve active manipulation of spin photons in multiple dimensions, and the developed device might find potential applications in various areas, e.g., optical microscopy

Dynamic Responses of the Cylindrical Floating Drilling Production Storage and Offloading System with Annular Anti-Motion Structures under the Survival Sea Scenario

At present, dry wellheads are usually adopted on small-storage TLP and SPAR platforms to develop offshore oil and gas because of the robust hydrodynamic performance under severe-wind seas. On the other hand, FPSO and FDPSO platforms that have a larger storage capacity can hardly use this cost-saving facility due to their relatively poor vertical motion performance. Cylindrical FPSOs are proposed to improve the heave performance of ship-type FPSOs, but their behaviors are still too large to adopt the dry wellheads. In the present work, a cylindrical FDPSO platform is proposed based on the FWPSO platform, adding an extension cylinder and a new damping structure at the bottom. Their hydrodynamic performances are calculated by the potential theory and compared in the frequency domain. Taking two particular mooring systems, including both catenary and ‘chain-polyester-chain’ types, and the survival sea scenario in the South China Sea into account, a time-domain coupling analysis was adopted to simulate the dynamic performance of the platform-mooring system. The feasibility of dry wellhead adoption on the FDPSO is discussed by investigating the platform motion and the mooring tension. The results show that the FCDS platform with the ‘chain-polyester-chain’-type mooring system can meet the motion response requirements, and the mooring system can also meet the requirements of the specification

Genomic characterization of a novel virus of the family Tymoviridae isolated from mosquitoes.

BACKGROUND: The family Tymoviridae comprises three plant virus genera, including Tymovirus, Marafivirus, and Maculavirus, which are found in most parts of the world and cause severe agricultural losses. We describe a putatively novel member of the family Tymoviridae, which is isolated from mosquitoes (Culex spp.), referred to as CuTLV. METHODS AND RESULTS: The CuTLV was isolated by cell culture, which replicates and causes cytopathic effects in Aedes albopictus C6/36 cells, but not in mammalian BHK-21 or Vero cells. The complete 6471 nucleotide sequence of CuTLV was determined. The genome of CuTLV is predicted to contain three open reading frames (ORFs). The largest ORF1 is 5307 nucleotides (nt) in length and encodes a putative polypeptide of 1769 amino acids (aa), which contains the conserved motifs for the methyltransferase (MTR), Tymovirus endopeptidase (PRO), helicase (HEL), and RNA-dependent RNA polymerase (RdRp) of the replication-associated proteins (RPs) of positive-stranded RNA viruses. In contrast, the ORF1 sequence does not contain the so-called "tymobox" or "marafibox", the conserved subgenomic RNA promoter present in all tymoviruses or marafiviruses, respectively. ORF2 and ORF3 putatively encode a 248-aa coat protein (CP) and a proline-rich 149-aa polypeptide. The whole genomic nucleotide identity of CuTLV with other members of family Tymoviridae ranged from 46.2% (ChiYMV) to 52.4% (GFkV). Phylogenetic analysis based on the putative RP and CP genes of CuTLV demonstrated that the virus is most closely related to viruses in the genus Maculavirus. CONCLUSIONS: The CuTLV is a novel virus related to members of the family Tymoviridae, with molecular characters that are distinct from those of tymoviruses, marafiviruses, and other maculaviruses or macula-like viruses. This is the first report of the isolation of a Tymoviridae-like virus from mosquitoes. Further investigations are required to clarify the origin, replication strategy, and the public health or agricultural importance of the CuTLV