Using ring samples to evaluate the processing characteristics in high-pressure torsion

An Al–3% Mg–0.2% Sc alloy was processed by high-pressure torsion (HPT) using samples in the form of solid disks and rings. Following HPT, all values of the measured Vickers microhardness fall onto a single curve when plotted against the equivalent strain, such that there are increasing values of hardness at the lower strains and hardness saturation above equivalent strains of ~40. This saturation level is independent of the number of revolutions and the applied pressure. The grain size following HPT is ~220 nm, and tensile tests show that the material is superplastic at a testing temperature of 573 K

Microstructural and mechanical characteristics of an AZ61 magnesium alloy processed by high pressure torsion

Experiments were conducted on an AZ61 magnesium alloy to evaluate the microstructural characteristics and the mechanical properties after processing by High-Pressure Torsion (HPT). The results show that processing by HPT produces excellent grain refinement with average grain sizes of ~0.22 and ~0.11 µm after processing at 423 K and room temperature, respectively. Tensile testing after HPT revealed the potential for achieving superplastic elongations with a maximum recorded elongation of 620% when testing at a temperature of 473 K. Using microhardness measurements, it is demonstrated that the microstructure gradually evolves with increasing torsional straining in HPT so that ultimately there is a reasonably homogeneous structure across the disk

PTEN-induced kinase 1 gene single-nucleotide variants as biomarkers in adjuvant chemotherapy for colorectal cancer: a retrospective study

Abstract Background Fluoropyrimidine-based postoperative adjuvant chemotherapy is globally recommended for high-risk stage II and stage III colon cancer. However, adjuvant chemotherapy is often associated with severe adverse events and is not highly effective in preventing recurrence. Therefore, discovery of novel molecular biomarkers of postoperative adjuvant chemotherapy to identify patients at increased risk of recurrent colorectal cancer is warranted. Autophagy (including mitophagy) is activated under chemotherapy-induced stress and contributes to chemotherapy resistance. Expression of autophagy-related genes and their single-nucleotide polymorphisms are reported to be effective predictors of chemotherapy response in some cancers. Our goal was to evaluate the relationship between single-nucleotide variants of autophagy-related genes and recurrence rates in order to identify novel biomarkers that predict the effect of adjuvant chemotherapy in colorectal cancer. Methods We analyzed surgical or biopsy specimens from 84 patients who underwent radical surgery followed by fluoropyrimidine-based adjuvant chemotherapy at Saitama Medical University International Medical Center between January and December 2016. Using targeted enrichment sequencing, we identified single-nucleotide variants and insertions/deletions in 50 genes, including autophagy-related genes, and examined their association with colorectal cancer recurrence rates. Results We detected 560 single-nucleotide variants and insertions/deletions in the target region. The results of Fisher’s exact test indicated that the recurrence rate of colorectal cancer after adjuvant chemotherapy was significantly lower in patients with the single-nucleotide variants (c.1018G > A [p  C [p < 0.01]) of the mitophagy-related gene PTEN-induced kinase 1. Conclusions The two single-nucleotide variants of PINK1 gene may be biomarkers of non-recurrence in colorectal cancer patients who received postoperative adjuvant chemotherapy