Appearance of vascular endothelial growth factor (VEGF) in femoral head in the growing rat

In this study, we examined the appearance of vascular endothelial growth factor (VEGF) in the femoral head of the growing rat using an immunocytochemical technique. Our results showed VEGF-immunopositive cells existed in the inner region and peripheral region of the femoral head at each developmental stage. In the 19-day-old fetus, immunopositive mesenchymal cells were demonstrated in the peripheral region of the femoral head. At 1 to 10 days after birth, VEGF immunoreactivities were observed in the osteoblasts, osteoclasts, periosteum, perichondrium and cartilage matrix of the femur. At 15 days after birth, VEGF immunoreactive chondrocytes appeared in the apex area of the femoral head. In this stage, the femoral head is still constituted by chondrocytes and no apparent vascular formation has been observed. Thereafter, the immunopositive chondrocytes in the femoral head increased in number. The penetration of capillaries was recognized within the ligament of the femoral head at 60 days after birth. The results indicate that some chondrocytes in the femoral head produce VEGF before the beginning of ossification, and that VEGF may play an important role in the penetration of blood vessels into the femoral head from the ligament of the femoral head

Selective Recovery of Indium from Acid Sulfate Media with Solvent Impregnated Resin of Bis(4-cyclohexylcyclohexyl)phosphoric Acid as an Extractant

ABSTRACT The selective recovery of In 3+ from the mixture of In 3+ , Ga 3+ , and Zn 2+ was investigated in acidic sulfate media, employing a solvent impregnated resin (SIR). Bis(4-cyclohexylcyclohexyl)phosophoric acid (D4DCHPA) in toluene was impregnated into synthetic adsorbent based on methacrylic ester copolymer. The SIR has high selectivity for In 3+ , and the order of selectivity is In 3+ > Ga 3+ > Zn 2+ . The adsorption progresses via Langmuir mechanism and the maximum adsorption amounts of In 3+ , Ga 3+ , and Zn 2+ are found to be 0.22, 0.22, and 0.14 mmol/g, respectively. The effective adsorption-elution can be achieved by the column operation, and In 3+ can be selectively recovered from ternary metal solution

Appearance of vascular endothelial growth factor (VEGF) in femoral head in the growing rat

In this study, we examined the appearance of vascular endothelial growth factor (VEGF) in the femoral head of the growing rat using an immunocytochemical technique. Our results showed VEGF-immunopositive cells existed in the inner region and peripheral region of the femoral head at each developmental stage. In the 19-day-old fetus, immunopositive mesenchymal cells were demonstrated in the peripheral region of the femoral head. At 1 to 10 days after birth, VEGF immunoreactivities were observed in the osteoblasts, osteoclasts, periosteum, perichondrium and cartilage matrix of the femur. At 15 days after birth, VEGF immunoreactive chondrocytes appeared in the apex area of the femoral head. In this stage, the femoral head is still constituted by chondrocytes and no apparent vascular formation has been observed. Thereafter, the immunopositive chondrocytes in the femoral head increased in number. The penetration of capillaries was recognized within the ligament of the femoral head at 60 days after birth. The results indicate that some chondrocytes in the femoral head produce VEGF before the beginning of ossification, and that VEGF may play an important role in the penetration of blood vessels into the femoral head from the ligament of the femoral head

Selective recovery of lithium from seawater using a novel MnO2 type adsorbent. II – Enhancement of lithium ion selectivity of the adsorbent

A novel spinel-type manganese dioxide adsorbent has been developed for the selective recovery of Li+ from seawater. The adsorbent can be prepared from a lithium-rich component of spinel-type lithium dimanganese tetraoxide Li1.5Mn2O4, followed by ion exchange of Li+ by H+ with diluted hydrochloric acid. The X-ray analysis of the adsorbent suggests the adsorption-elution cycle of Li+ progresses under γ expansion-shrinking mechanism of the adsorbent. The adsorption of Li+ progresses via cation exchange, and the selective recovery of Li+ can be carried out, even when the large amount of Na+ coexists in seawater. The chromatographic selective recovery of Li+ from the artificial seawater shows that Li+ can be selectively adsorbed with remaining most of Na+ in the feed solution in the break through step, while Li+ of high purity can recover and concentrate into the elutant in the elution step