Kaplan-Meier analysis for cardiovascular disease survival by sexual interest.

(A) males, (B) females.</p

Kaplan-Meier analysis for overall survival by sexual interest.

(A) males, (B) females.</p

Characteristics of study subjects.

Characteristics of study subjects.</p

Kaplan-Meier analysis for cancer survival by sexual interest.

(A) males, (B) females.</p

Sex-specific unadjusted, age-, and multivariable-adjusted HRs and 95% CIs of mortality according to lack of sexual interest.

Sex-specific unadjusted, age-, and multivariable-adjusted HRs and 95% CIs of mortality according to lack of sexual interest.</p

Predicted target genes of 9 differentially expressed miRNA by Illumina sequencing in PBC.

*<p>miRIS :miRror Internal Score ranges from 0 top 1 by average 2 components (number of databases and input hits).</p

Validation of deep sequencing results for selected miRNAs.

<p>We have registered 10 samples in each group listed on Table S3. The threshold cycle for each miRNA primer/probe set were normalized with spiked in cel-miR-39 primer/probe pair and compared to CH-C-5. Result for normally distributed continuous variables are given as means and compared between groups by Student's t-test. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066086#s2" target="_blank">Results</a> for non-normally distributed continuous variables are summarized as medians and were compared by Mann-Whitney U test. Statistical significance indicates by one asterisk (p<0.05) and two (p<0.01).</p

Clinical data for patients enrolled in Illumina sequencing analysis.

a<p>We used the Scheuer score in PBC and fibrosis score of histological activity index (HAI) in CH-B and CH-C <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066086#pone.0066086-Knodell1" target="_blank">[53]</a><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066086#pone.0066086-Ludwig1" target="_blank">[54]</a><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066086#pone.0066086-Scheuer1" target="_blank">[55]</a>.</p>b<p>URSO is the abbreviation for ursodeoxycholic acid, IFN for interferon and NA for nucleos(t)ide analogue.</p

Clinical information of patients enrolled in the validation study.

a<p>The numbers of patients are indicated in the parentheses.</p>b<p>The range of laboratory data is indicated in the parentheses.</p

The number of small RNAs in serum detected by Illumina sequencing.

<p>The number of small RNAs in serum detected by Illumina sequencing.</p