Additional file 2: of Genetic polymorphisms of HLA-DP and isolated anti-HBc are important subsets of occult hepatitis B infection in Indonesian blood donors: a case-control study

Ethical Clearance dr Widya 2014 (SECOND). (PDF 996 kb

Sero-prevalence of HBsAg, anti-HBc Ab, anti-HCV Ab and anti-GBV-C Ab among CSWs residing in Surabaya.^*

<p>*Plasma sample was tested for HBsAg, anti-HBc Ab, anti-HCV Ab and anti-GBV-C Ab using commertially available diagnostic kits.</p><p>**Plasma sample was tested for anti-HIV antibodies using a rapid diagnostic kit, and was then tested using 2 additional diagnostic kits to confirm the diagnosis of HIV infection.</p><p>***The sero-prevalence rate (%) among a group of CSWs indicated is shown in parentheses.</p

Phylogenetic relationship of HIV-1 <i>pol</i> and <i>env</i> gene sequences.

<p>Phylogenetic trees were generated for newly sequenced HIV-1 <i>pol</i> (A) and <i>env</i> (B) genes together with the corresponding viral gene of reference HIV-1 strains representing subtype A1 (A1), subtype A2 (A2), subtype B (B), subtype C (C), subtype D (D), CRF01_AE (AE), CRF02_AG (AG), CRF15_01B (15_01B), CRF33_01B (33_01B) and CRF34_01B (34_01B) (shown with a gray background). Bootstrap values are shown when the values are >50. Scale bar represents 0.01 (A) or 0.02 nucleotide substitutions per site (B). The nucleotide sequences of <i>pol</i> and <i>env</i> genes determined in this study have been deposited in the GenBank database under accession numbers KF147334-KF147375.</p

Viral subtype and CRF detected in the blood samples of HIV-1-positive CSWs.^*

<p>*HIV-1 <i>pol</i> and <i>env</i> genes were amplified and subjected to sequencing analysis.</p><p>Viral subtyping was carried out using the Recombinant Identification Program (RIP).</p><p>**HIV-1 <i>env</i> gene failed to be amplified; therefore, viral subtyping was not carried out.</p