1 research outputs found
Site-Specific Attachment of a Protein to a Carbon Nanotube End without Loss of Protein Function
Establishing a nanobiohybrid device largely relies on
the availability
of various bioconjugation procedures which allow coupling of biomolecules
and inorganic materials. Especially, site-specific coupling of a protein
to nanomaterials is highly useful and significant, since it can avoid
adversely affecting the protein’s function. In this study,
we demonstrated a covalent coupling of a protein of interest to the
end of carbon nanotubes without affecting protein’s function.
A modified Staudinger-Bertozzi ligation was utilized to couple a carbon
nanotube end with an azide group which is site-specifically incorporated
into a protein of interest. We demonstrated that Ca<sup>2+</sup>-sensor
protein, calmodulin, can be attached to the end of the nanotubes without
affecting the ability to bind to the substrate in a calcium-dependent
manner. This procedure can be applied not only to nanotubes, but also
to other nanomaterials, and therefore provides a fundamental technique
for well-controlled protein conjugation