Assessment of epigenetic alterations in early colorectal lesions containing BRAF mutations

金沢大学医薬保健学総合研究科先進的地域医療研究講座 = Department of Advanced Research in Community MedicineTo clarify the molecular and clinicopathological characteristics of colorectal serrated lesions, we assessed the DNA methylation of cancer-associated genes in a cohort of BRAF-mutant precancerous lesions from 94 individuals. We then compared those results with the lesions’ clinicopathological features, especially colorectal subsites. The lesions included hyperplastic polyps (n = 16), traditional serrated adenomas (TSAs) (n = 15), TSAs with sessile serrated adenomas (SSAs) (n = 6), SSAs (n = 49) and SSAs with dysplasia (n = 16). The prevalence of lesions exhibiting the CpG island methylator phenotype (CIMP) was lower in the sigmoid colon and rectum than in other bowel subsites, including the cecum, ascending, transverse and descending colon. In addition, several cancer-associated genes showed higher methylation levels within lesions in the proximal to sigmoid colon than in the sigmoid colon and rectum. These results indicate that the methylation status of lesions with BRAF mutation is strongly associated with their location, histological findings and neoplastic pathways. By contrast, no difference in aberrant DNA methylation was observed in normal-appearing background colonic mucosa along the bowel subsites, which may indicate the absence of an epigenetic field defect

Application of stabilized hypobromite for controlling membrane fouling and N-nitrosodimethylamine formation

Chloramination is a conventional and successful pre-disinfection approach to control biological fouling for reverse osmosis (RO) treatment in water reuse. This study aimed to evaluate the possibility of using a new disinfectant?stabilized hypobromite?in controlling membrane fouling and the formation of a particular carcinogenic disinfection byproduct (DBP)?N-nitrosodimethylamine (NDMA). Our accelerated chemical exposure tests showed that the new disinfectant reduced the permeability of a polyamide RO membrane permeability from 6.7 to 4.1 L/m2hbar; however, its treatment impact was equivalent to that of chloramine. The disinfection efficacy of stabilized hypobromite was greater than that of chloramine when evaluated with intact bacterial counts, which suggests its potential for mitigating membrane biofouling. Additional pilot-scale tests using synthetic wastewater demonstrated that pre-disinfection with the use of stabilized hypobromite inhibits membrane fouling. Among 13 halogenated DBPs evaluated, the formation of bromoform by stabilized hypobromite was higher than that by chloramine at a high dose of 10 mg/L, thus suggesting the need for optimizing chemical doses for achieving sufficient biofouling mitigation. NDMA formation upon stabilized hypobromite treatment in two different types of actual treated wastewaters was found to be negligible and considerably lower than that by chloramine treatment. In addition, NDMA formation potential by stabilized hypobromite was 2?5 orders of magnitude lower than that by chloramine. Our findings suggest the potential of using stabilized hypobromite for controlling NDMA formation and biofouling, which are the keys to successful potable water reuse

Weaving a colorful cloth: Centering education on humans’ emergent developmental potentials

We integrate work from human development, psychology, education, and neuroscience to argue for five interrelated developmental principles that together provide the conceptual basis for a fundamental shift in thinking in education about the nature of learning, and hence the work of teaching, and the purpose and design of schools and youth-facing policies. These principles foreground humans’ natural agency, subjectivity, and variability, and the dynamic, adaptive interdependence of body, mind, and culture in development and learning. We take the analogy of weaving cloth to highlight the properties and valuable variations of effective educational systems. We argue that reconceptualizing learning is necessary to meaningfully improve schooling and its outcomes, support equity and human dignity, and, ultimately, to build a sustainable democratic society

Pokémon GO and psychological distress, physical complaints, and work performance among adult workers:a retrospective cohort study

Abstract The effects of Pokémon GO, a new mobile game application which utilizes augmented reality, on risky behavior and health have already been discussed in anecdotal evidence. However, there have been no studies about its effects on mental health. This study investigated the relationships between Pokémon GO and psychological distress from an existing workers’ cohort in Japan. Online surveys were conducted to 3,915 full-time workers, at baseline (Nov 26, 2015–Feb 18, 2016) and at follow-up (Dec 1–4, 2016), using a self-report questionnaire. Pokémon GO players were defined as participants who had played Pokémon GO for one month or longer. Psychological distress was measured using validated scales. Of the completers, 246 (9.7%) had continued to play Pokémon GO. They were significantly younger than non-players. From the results of the general linear modeling, improvement in psychological distress was significantly greater among Pokémon GO players than among non-players (p = 0.025). Cohen’s d for the difference in psychological distress was −0.20 (95% CI, −0.33, −0.07). Pokémon GO may be effective for improving psychological distress among workers. Although its effect size is small, the game could have positive effects on the mental health of the adult working population

Effect of the National Stress Check Program on mental health among workers in Japan:A 1-year retrospective cohort study

OBJECTIVES: This retrospective cohort study evaluated the impact of the Stress Check Program, a recently introduced national policy and program aimed at reducing psychological distress among Japanese workers. METHODS: A baseline survey was conducted from November 2015 to February 2016, the period when Japan began enforcing the Stress Check Program. A one-year follow-up survey was conducted in December 2016. In the follow-up survey, two exposure variables were collected: having taken the annual stress survey, and experiencing an improvement in the psychosocial work environment. Psychological distress was assessed using the Brief Job Stress Questionnaire (BJSQ) at baseline and 1-year follow-up. The two exposure variables were used to define four groups: "Neither", "Stress survey (SS) only", "Psychosocial work environment improvement (WI) only", and "Both". BJSQ results were analyzed using repeated measures general linear modeling (GLM). RESULTS: The study included 2,492 participants: 1,342 in the "Neither" group, 1,009 in the "SS only" group, 76 in the "WI only" group, and 65 in the "Both" group. Overall time-group interaction effects were not significant. The "Both" group showed significantly greater improvements in psychological distress than the "Neither" group (p = 0.02) at the 1-year follow-up, although the effect size was small (d = -0.14). CONCLUSIONS: Combination of the annual stress survey and improvement in psychosocial work environment may have been effective in reducing psychological distress in workers, although the effect size was small

Controlling biofouling and disinfection by-product formation during reverse osmosis treatment for seawater desalination

Controlling membrane fouling and disinfection by-products (DBPs) is an ongoing challenge in achieving sustainable membrane-based seawater desalination. This study assessed the efficacy of a new disinfectant, stabilized hypobromite, for controlling biofouling and DBP formation during reverse osmosis (RO) membrane treatment of seawater. Accelerated chemical exposure tests revealed that stabilized hypobromite did not degrade a commercial polyamide RO membrane; thus, unlike other powerful oxidants, it is able to remain as a residual chemical on membrane surfaces. In our experiments, stabilized hypobromite also effectively inactivated bacteria in seawater and reduced potential organic foulants (e.g., humic acid-like and protein-like substances). Disinfection at a stabilized hypobromite dose of 5 mg-Cl2/L resulted in the formation of trihalomethanes (THMs), haloacetic acids (HAAs), and bromate at 55, 29, and <10 μg/L, respectively. Chlorine treatment resulted in higher formations of THMs, HAAs, and bromate (80, 74, and 50 μg/L, respectively), indicating stabilized hypobromite is superior to chlorine in this respect. Pilot-scale validation demonstrated that pre-disinfection with stabilized hypobromite enabled the RO membrane treatment to operate for half a year without significant fouling. The findings in this study indicate the great potential of stabilized hypobromite for controlling DBP formation and biofouling in seawater desalination