Background contributions in the electron-tracking Compton camera onboard SMILE-2+

The Mega electron volt (MeV) gamma-ray observation is a promising diagnostic tool for observing the universe. However, the sensitivity of MeV gamma-ray telescopes is limited due to peculiar backgrounds, restricting the application of MeV gamma rays for observation. Identification of backgrounds is crucial for designing next-generation telescopes. Therefore, herein, we assessed the background contribution in the electron-tracking Compton camera (ETCC) on board the SMILE- 2+ balloon experiment. This assessment was performed using the Monte Carlo simulation. The results revealed that the background below 400 keV existed due to the atmospheric gamma-ray background, the cosmic-ray/secondary-particle background, and the accidental background. On the other hand, the unresolved background component, which was not likely to be relevant to direct Compton-scattering events in the ETCC, was confirmed above 400 keV. Overall, this study demonstrated that the Compton-kinematics test provides a powerful tool to remove the background and principally improves the signal-to-noise ratio at 400 keV by an order of magnitude.Comment: 11 pages, 18 figure

Development of Convolutional Neural Networks for an Electron-Tracking Compton Camera

Electron-tracking Compton camera, which is a complete Compton camera with tracking Compton scattering electron by a gas micro time projection chamber, is expected to open up MeV gamma-ray astronomy. The technical challenge for achieving several degrees of the point spread function is the precise determination of the electron-recoil direction and the scattering position from track images. We attempted to reconstruct these parameters using convolutional neural networks. Two network models were designed to predict the recoil direction and the scattering position. These models marked 41$~$degrees of the angular resolution and 2.1$~$mm of the position resolution for 75$~$keV electron simulation data in Argon-based gas at 2$~$atm pressure. In addition, the point spread function of ETCC was improved to 15$~$degrees from 22$~$degrees for experimental data of 662$~$keV gamma-ray source. These performances greatly surpassed that using the traditional analysis

How to Visualize the Work of Historical Understanding and Thinking: Introductory History Lesson “Morning Glory Boom in the Edo Period” with Thinking Tools

The aim of this research is to answer the following questions: “How should we teach historical understanding and thinking and visualize the work of historical understanding and thinking?” This research focuses on instruction that encourages students to engage in understanding and thinking. To achieve this, this research developed three principles for teaching. Based on these, we developed an introductory history lesson “Morning Glory Boom in the Edo Period”. This lesson was taught to first year students at a junior high school. The results show that by using appropriate thinking tools in the process of forming historical recognition, we can understand the steps involved in a student’s historical understanding and thinking. In addition, as result of visualizing the student’s historical understanding and thinking, we can improve lessons, taking into account their limitations

The Principal of Inquiry-based Social Studies Curriculum for Developing Children’s Competency and Its Example Units: A Case of “The College, Career, and Civic Life (C3) Framework” by NCSS

This paper aims at answering the following two questions; one is “How can social studies move from contents-focused to contents-skill-balanced?” and the other is “How can social studies’ unique skills and competency that extend beyond the boundary of individual subject be cultivated simultaneously?” To answer these questions, authors analyzed “The College, Career, and Civic Life (C3) Framework” and its four example units, which are designed by NCSS to promote inquiry-based curriculum and cultivating competency within social studies. The four example units were based on the “Inquiry Arc,” which is the core of the framework: (a) developing questions and planning inquiries, (b) applying disciplinary tools and concepts, (c) evaluating sources and using evidence, and (d) communicating conclusions and taking informed action. After the analysis, authors could discover the framework’s three characteristics. (1) C3 Framework integrates social science disciplines into social studies by using them as lenses to understand society. (2) The framework also achieves a good balance between doing social studies and developing competencies that goes beyond a subject through adapting Inquiry Arc. (3) The framework gives discretionary power to curriculum designers including teachers, so they should be in charge of their curriculum as gatekeepers

First observation of MeV gamma-ray universe with bijective imaging spectroscopy using the Electron-Tracking Compton Telescope aboard SMILE-2+

MeV gamma-rays provide a unique window for the direct measurement of line emissions from radioisotopes, but observations have made little significant progress after COMPTEL/{\it CGRO}. To observe celestial objects in this band, we are developing an electron-tracking Compton camera (ETCC), which realizes both bijective imaging spectroscopy and efficient background reduction gleaned from the recoil electron track information. The energy spectrum of the observation target can then be obtained by a simple ON-OFF method using a correctly defined point spread function on the celestial sphere. The performance of celestial object observations was validated on the second balloon SMILE-2+ installed with an ETCC having a gaseous electron tracker with a volume of 30$\times$30$\times$30 cm$^3$. Gamma-rays from the Crab nebula were detected with a significance of 4.0$\sigma$ in the energy range 0.15--2.1 MeV with a live time of 5.1 h, as expected before launching. Additionally, the light curve clarified an enhancement of gamma-ray events generated in the Galactic center region, indicating that a significant proportion of the final remaining events are cosmic gamma rays. Independently, the observed intensity and time variation were consistent with the pre-launch estimates except in the Galactic center region. The estimates were based on the total background of extragalactic diffuse, atmospheric, and instrumental gamma-rays after accounting for the variations in the atmospheric depth and rigidity during the level flight. The Crab results and light curve strongly support our understanding of both the detection sensitivity and the background in real observations. This work promises significant advances in MeV gamma-ray astronomy

Epithelial EP4 plays an essential role in maintaining homeostasis in colon

Colonic epithelial cells comprise the mucosal barrier, and their dysfunction promotes microbial invasion from the gut lumen and induces the development of intestinal inflammation. The EP4 receptor is known to mediate the protective effect of prostaglandin (PG) E2 in the gastrointestinal tract; however, the exact role of epithelial EP4 in intestinal pathophysiology remains unknown. In the present study, we aimed to investigate the role of epithelial EP4 in maintaining colonic homeostasis by characterizing the intestinal epithelial cell-specific EP4 knockout (EP4 cKO) mice. Mice harboring the epithelial EP4 deletion showed significantly lower colonic crypt depth and lower numbers of secretory cell lineages, as well as impaired epithelial cells in the colon. Interestingly, EP4-deficient colon epithelia showed a higher number of apoptotic cells. Consistent with the defect in mucosal barrier function of colonic epithelia and secretory cell lineages, EP4 cKO colon stroma showed enhanced immune cell infiltration, which was accompanied by increased production of inflammatory cytokines. Furthermore, EP4-deficient colons were susceptible to dextran sulfate sodium (DSS)-induced colitis. Our study is the first to demonstrate that epithelial EP4 loss resulted in potential "inflammatory" status under physiological conditions. These findings provided insights into the crucial role of epithelial PGE2/EP4 axis in maintaining intestinal homeostasis

Breakdown of lung framework and an increase in pores of Kohn as initial events of emphysema and a cause of reduction in diffusing capacity

Purpose: Pulmonary emphysema is the pathological prototype of chronic obstructive pulmonary disease and is also associated with other lung diseases. We considered that observation with different approaches may provide new insights for the pathogenesis of emphysema. Patients and methods: We reviewed tissue blocks of the lungs of 25 cases with/without emphysema and applied a three-dimensional observation method to the blocks. Based on the three-dimensional characteristics of the alveolar structure, we considered one face of the alveolar polyhedron as a structural unit of alveoli and called it a framework unit (FU). We categorized FUs based on their morphological characteristics and counted their number to evaluate the destructive changes in alveoli. We also evaluated the number and the area of pores of Kohn in FUs. We performed linear regression analysis to estimate the effect of these data on pulmonary function tests. Results: In multivariable regression analysis, a decrease in the number of FUs without an alveolar wall led to a significant decrease in the diffusing capacity of the lung for carbon monoxide (DLCO) and DLCO per unit alveolar volume, and an increase in the area of pores of Kohn had a significant effect on an increase in residual capacity. Conclusion: A breakdown in the lung framework and an increase in pores of Kohn are associated with a decrease in DLCO and DLCO per unit alveolar volume with/without emphysema

Cdh23 and Prepulse Inhibition

We previously identified quantitative trait loci (QTL) for prepulse inhibition (PPI), an endophenotype of schizophrenia, on mouse chromosome 10 and reported Fabp7 as a candidate gene from an analysis of F2 mice from inbred strains with high (C57BL/6N; B6) and low (C3H/HeN; C3H) PPI levels. Here, we reanalyzed the previously reported QTLs with increased marker density. The highest logarithm of odds score (26.66) peaked at a synonymous coding and splice-site variant, c.753G>A (rs257098870), in the Cdh23 gene on chromosome 10; the c.753G (C3H) allele showed a PPI-lowering effect. Bayesian multiple QTL mapping also supported the same variant with a posterior probability of 1. Thus, we engineered the c.753G (C3H) allele into the B6 genetic background, which led to dampened PPI. We also revealed an e-QTL (expression QTL) effect imparted by the c.753G>A variant for the Cdh23 expression in the brain. In a human study, a homologous variant (c.753G>A; rs769896655) in CDH23 showed a nominally significant enrichment in individuals with schizophrenia. We also identified multiple potentially deleterious CDH23 variants in individuals with schizophrenia. Collectively, the present study reveals a PPI-regulating Cdh23 variant and a possible contribution of CDH23 to schizophrenia susceptibility

JNK pathway plays a critical role for expansion of human colorectal cancer in the context of BRG1 suppression

Tumor stem cells (TSCs), capable of self-renewal and continuous production of progeny cells, could be potential therapeutic targets. We have recently reported that chromatin remodeling regulator Brg1 is required for maintenance of murine intestinal TSCs and stemness feature of human colorectal cancer (CRC) cells by inhibiting apoptosis. However, it is still unclear how BRG1 suppression changes the underlying intracellular mechanisms of human CRC cells. We found that Brg1 suppression resulted in upregulation of the JNK signaling pathway in human CRC cells and murine intestinal TSCs. Simultaneous suppression of BRG1 and the JNK pathway, either by pharmacological inhibition or silencing of c-JUN, resulted in even stronger inhibition of the expansion of human CRC cells compared to Brg1 suppression alone. Consistently, high c-JUN expression correlated with worse prognosis for survival in human CRC patients with low BRG1 expression. Therefore, the JNK pathway plays a critical role for expansion and stemness of human CRC cells in the context of BRG1 suppression, and thus a combined blockade of BRG1 and the JNK pathway could be a novel therapeutic approach against human CRC