Univariate and multivariate analysis of factors for breast cancer-specific survival.

<p>Univariate and multivariate analysis of factors for breast cancer-specific survival.</p

Univariate and multivariate analysis of factors for distant relapse-free survival.

<p>Univariate and multivariate analysis of factors for distant relapse-free survival.</p

Univariate and multivariate analysis of factors for relapse-free survival.

<p>Univariate and multivariate analysis of factors for relapse-free survival.</p

Representative immunostaining of CD8, CD68, and CD169.

<p>Tumor infiltrating CD8⁺ lymphocytes were evaluated in primary cancer tissues, while CD68⁺ and CD169⁺ sinus macrophages were evaluated in RLNs (A). Representative images of CD8 (B) and of CD68 and CD169 (C) immunostaining. HE, Hematoxylin-Eosin staining.</p

Statistical analysis of associations of sinus macrophages with clinicopathological factors.

<p>Analysis of the association of CD169⁺ macrophage density and the CD169⁺/CD68⁺ macrophage ratio with LN metastasis (A), clinical stage (B), the Ki-67 index (C) and prognosis (D). The Mann-Whitney U test and Kruskal-Wallis tests were performed to examine the prognostic value of CD169 in (D).</p

Statistical analysis of associations of CD8⁺ lymphocytes with CD169⁺ macrophages and clinicopathological factors.

<p>Analysis of the association of CD8⁺ lymphocyte density with CD169⁺ macrophage density (upper) and the CD169⁺/CD68⁺ macrophage ratio (lower) (A) and with LN metastasis (B) in all cases. Similar analyses to (A) and (B) that were performed in cases with a high Ki-67 index (>40%) are shown in (C) and (D), respectively.</p

miR-30e* suppresses Bmi1 expression in gastrointestinal cells.

<p>(<b>A</b>) Western blot analysis of Bmi1 expression in 6 gastrointestinal cancer cell lines. (<b>B</b>) Western blot analysis of Bmi1 expression in high Bmi1-expressing AGS cell lines transfected with negative control (NC) and miR-30e* mimics. (<b>C</b>) Western blot analysis of Bmi1 expression in high Bmi1-expressing HCT116 cell lines transfected with NC and miR-30e* mimics. (<b>D</b>) Western blot analysis of Bmi1 expression in low Bmi1-expressing NUGC4 cell lines transfected with NC and miR-30e* inhibitors. (<b>E</b>) Western blot analysis of Bmi1 expression in low Bmi1-expressing COLO201 cell lines transfected with NC and miR-30e* inhibitors.</p

miR-30e* expression in human gastrointestinal cancer tissues.

<p>(<b>A</b>) The levels of miR-30e* expression in 16 gastric cancer tissues and their matched adjacent normal gastric epithelia as assessed by qRT-PCR. (<b>B</b>) The levels of miR-30e* expression in 29 of high and 24 of low Bmi1-expressing gastric cancer tissues as assessed by qRT-PCR. (<b>C</b>) The levels of miR-30e* expression in 37 colon cancer tissues and their matched adjacent normal colon epithelia as assessed by qRT-PCR. (<b>D</b>) The levels of miR-30e* expression in 20 of high and 17 of low Bmi1-expressing colon cancer tissues as assessed by qRT-PCR.</p

Association of CD169⁺, CD68⁺, and CD8⁺expressing cells with clinicopathological parameters in the invasive breast cancer cohort.

<p>Association of CD169⁺, CD68⁺, and CD8⁺expressing cells with clinicopathological parameters in the invasive breast cancer cohort.</p

Expression of miR-30e* and Bmi1 co-cultured with M1- and M2-polarized macrophages purified from human monocytes.

<p>(<b>A</b>) qRT-PCR analysis of miR-30e* expression in AGS cells co-cultured with M1- and M2-polarized macrophages. Significantly lower miR-30e* expression was detected in co-cultured groups compared with the control group (***<i>P</i> < 0.001, *<i>P</i> < 0.05, respectively). (<b>B</b>) qRT-PCR analysis of miR-30e* expression in HCT116 cells co-cultured with M1- and M2-polarized macrophages. Significantly lower miR-30e* expression was detected in co-cultured groups compared with the control group (***<i>P</i> < 0.001, **<i>P</i> < 0.01, respectively). (<b>C</b>) qRT-PCR analysis of Bmi1 expression in AGS cells co-cultured with M1- and M2-polarized macrophages. Significantly higher Bmi1 expression was detected in co-cultured groups compared with the control group (***<i>P</i> < 0.001, **<i>P</i> < 0.01, respectively). (<b>D</b>) qRT-PCR analysis of miR-30e* expression in HCT116 cells co-cultured with M1- and M2-polarized macrophages. Significantly higher Bmi1 expression was detected in M1 macrophage co-cultured groups compared with the control group (**<i>P</i> < 0.01). (<b>E</b>) Schematic representation of miR-30e*-Bmi1 signaling mediated by TAMs. </p