PATHOGENIC VARIABILITY OF ASCOCHYTA LENTIS IN BULGARIA

Ascochyta blight, caused by Ascochyta lentis Vassiljevsky, is an important foliar disease of lentil (Lens culinaris L.) with worldwide occurrence and is a serious threat to lentil production. The investigation involved 13 isolates of Ascochyta lentis. The pathogen was isolated from naturally infected lentil plants from various locations in Bulgaria, using an agar plate method. Inoculum was also producedby growing  isolates on LDA. Ten plants (3 weeks old) of each of the cultivars were sprayed with a freshly prepared spore suspension (105 conidia mL-1). Disease symptoms were scored 14 days after inoculation. The virulence of the isolates was determined with the help of 11 lines and cultivars of lentil (ILL 358, ILL 5480, ILL 2429, ILL 5725, ILL 7537, Laird, Ilina, Bella, Nadejda, Naslada, Zornitsa). Based on the reaction of the used genotypes, the investigated isolates of Asc. lentis were grouped into seven pathotypes. The observations in the present study revealed a significant variation amongthe isolates of Ascochyta lentis for morphological traits as well as for pathogenicity

Microbial prediction of metabolism of toluene in the environment

Abstract: The aim of this work is to predict the possible microbial metabolism of toluene by a software of (Q)SAR Application Toolbox. The toluene was metabolically activated in the microorganisms and their protein and DNA binding was estimated. Observed and predicted microbial pathways were observed. Observed and predicted metabolites have different mechanisms of protein binding

Concordance of Adherence Measurement Using Self-Reported Adherence Questionnaires and Medication Monitoring Devices

The primary objective of this review was to identify and examine the literature on the association between medication adherence self-reported questionnaires (SRQs) and medication monitoring devices. The primary literature search was performed for 1980-2009 using PubMed, PubMed In Process and Non-Indexed, Ovid MEDLINE, Ovid MEDLINE In-Process, PsycINFO (EBSCO), CINAHL (EBSCO), Ovid HealthStar, EMBASE (Elsevier) and Cochrane Databases and using the following search terms: 'patient compliance', 'medication adherence', 'treatment compliance', 'drug monitoring', 'drug therapy', 'electronic', 'digital', 'computer', 'monitor', 'monitoring', 'drug', 'drugs', 'pharmaceutical preparations', 'compliance' and 'medications'. We identified studies that included SRQs and electronic monitoring devices to measure adherence and focused on the SRQs that were found to be moderately to highly correlated with the monitoring devices. Of the 1679 citations found via the primary search, 41 full-text articles were reviewed for correlation between monitoring devices and SRQs. A majority (68%) of articles reported high (27%), moderate (29%) or significant (12%) correlation between monitoring devices (37 using Medication Event Monitoring System [MEMS] and four using other devices) and SRQs (11 identified and numerous other unnamed SRQs). The most commonly used SRQs were the Adult/Pediatric AIDS Clinical Trial Group (AACTG/PACTG; 24.4%, 10/41) followed by the 4-item Morisky (9.8%, 4/41), Brief Medication Questionnaire (9.8%, 4/41) and visual analogue scale (VAS; 7.3%, 3/41). Although study designs differed across the articles, SRQs appeared to report a higher rate of medication adherence (+14.9%) than monitoring devices. In conclusion, several medication adherence SRQs were validated using electronic monitoring devices. A majority of them showed high or moderate correlation with medication adherence measured using monitoring devices, and could be considered for measuring patient-reported adherence prospectively.Patient-compliance, Questionnaires.