Percentage of oocyte donors at each stage detected by (A) RIA or (B) ECLI methods using P4 concentrations of 4–8 ng/mL or (C) 6–15 ng/mL as ovulation standards.

<p>Pre: preovulation, IM, immature, M, mature and A, aging. Thirty dogs were used for analysis in each group.</p

Two canines derived from SCNT.

<p>(A) Canine derived using RIA and (B) ECLI methods.</p

Canine cloned using <i>in vivo</i> matured oocytes as predicted by RIA and ECLI methods.

<p>Canine cloned using <i>in vivo</i> matured oocytes as predicted by RIA and ECLI methods.</p

Classification of canine oocytes.

<p>Oocytes were categorized as immature, mature, and aged.</p

Comparison of progesterone concentrations (ng/mL) based on RIA and ECLI methods.

<p>(a'–j') Samples were separated based on the RIA data. Colocalization of RIA and ECLI methods were evaluated using the Pearson correlation coefficient. B) Average of RIA and ECLI methods. Data are presented as mean ± SD. The asterisk denotes significant differences (P<0.05).</p