Vehicle steering wheel angle identification research based on dynamic program method

In order to improve the safety of vehicle manipulation as well as to provide a theoretical basis for the study of vehicle steering system and intelligent parking systems, a new method of vehicle steering wheel angle identification is presented – the dynamic program method. First, three freedom degrees of vehicle model is established. Then Bellman’s principle of optimality is used for minimizing the objective function. The dynamic optimization model of the load identification that the yawing angular velocity, lateral acceleration and vehicle body roll angle identified the steering wheel angle and angle velocity. The result shows that the dynamic program method for the steering wheel angle identification problem containing the measurement noise has strong adaptability, high accuracy and good anti-jamming capability

Upregulation of tetraspanin 8 may contribute to LPSinduced acute lung injury by activation of the MAPK and NF-κB pathways

Purpose: To investigate the effect of tetraspanin8 (Tspan8, also known as TM4SF3 or CO-029) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the related signaling pathways.Methods: Treatment with LPS was used to induce lung damage in mice and a lung epithelial cell line. The wet-to-dry weight ratio of lung tissue, hematoxylin and eosin (H&E) staining, and quantification of cytokine concentrations were conducted to validate the model. Enzyme-linked immunosorbent assays (ELISA) and quantitative polymerase chain reaction (qPCR) were used to measure levels of tumor necrosis factor alpha, interleukin (IL)-1β, and IL-6. Tspan8 levels were knocked down using shRNAs. Mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) pathway levels were assessed after LPS-induced injury in this cellular model.Results: Levels of Tspan8 were upregulated in the LPS-induced ALI model. Additionally, LPS treatment of mouse lung epithelial cells resulted in Tspan8 upregulation. Tspan8 knockdown alleviated the effects of LPS on lung epithelial injury by inhibiting the upregulation of MAPK and NF-κB signaling pathways.Conclusion: The upregulation of Tspan8 may promote the progression of ALI

Vibration performance analysis of vehicle with the non-pneumatic new mechanical elastic wheel in the impulse input experiment

A non-pneumatic tire appears to have advantages over the conventional pneumatic tire in terms of flat proof and maintenance free. A mechanical elastic wheel (MEW) with a non-pneumatic elastic outer ring which functions as the air of the pneumatic tire was presented. The structure of MEW is non-inflatable integrated configuration and the effect of hinges is accounted for only in tension. To establish finite element model of MEW, various nonlinear factors, such as the geometrical nonlinearity, material nonlinearity and contact nonlinearity, were considered. Load characteristic test was conducted by tyre dynamic test-bed to obtain force-deflection curves. And the validity of the finite element model was validated through load characteristic test. The vehicle vibration performance respectively based on the MEW and the radial tire was compatative studied under pulse input experiment conditions. The result showed that the vehicle concluding the new mechanical elastic wheel met the vibration performance requirements and vibration performance regularity of pneumatic tire. The results could be used as the thesis reference for the improvement of new mechanical elastic wheel

Population dynamics of Brachionus calyciflorus driven by the associated natural bacterioplankton

Zooplankton provides bacteria with a complex microhabitat richen in organic and inorganic nutrients, and the bacteria community also changes the physiochemical conditions for zooplankton, where the symbiotic relationship between them plays an important role in the nutrient cycle. However, there are few studies on the effect of associated bacteria on the population dynamics of rotifers. In order to make clear their relationships, we reconstructed the associated bacterial community in Brachionus calyciflorus culture, and examined the life history and population growth parameters, and analyzed the diversity and community composition of the associated bacteria at different growth stages of B. calyciflorus. The results showed that the addition of bacteria from natural water can promote the population growth and asexual reproduction of B. calyciflorus, but has no significant effect on sexual reproduction, exhibited by the improvement of its life expectancy at hatching, net reproduction rates and intrinsic growth rate, no significant effects on the generation time and mixis ratio of offspring. It was found that the B. calyciflorus-associated bacterial community was mainly composed of Proteobacteria, Bacteroidota, Actinobacteriota, Cyanobacteria and Firmicutes. Through correlation network analysis, the members of Burkholderiales, Pseudomonadales, Micrococcales, Caulobacterales and Bifidobacteriales were the keystone taxa of B. calyciflorus-associated bacteria. In addition, the relative abundance of some specific bacteria strains increased as the population density of B. calyciflorus increased, such as Hydrogenophaga, Acidovorax, Flavobacterium, Rheinheimera, Novosphingobium and Limnobacter, and their relative abundance increased obviously during the slow and exponential phases of population growth. Meanwhile, the relative abundance of adverse taxa (such as Elizabethkingia and Rickettsiales) decreased significantly with the increase in rotifer population density. In conclusion, the closely associated bacteria are not sufficient for the best growth of B. calyciflorus, and external bacterioplankton is necessary. Furthermore, the function of keystone and rare taxa is necessary for further exploration. The investigation of the symbiotic relationship between zooplankton-associated bacterial and bacterioplankton communities will contribute to monitoring their roles in freshwater ecosystems, and regulate the population dynamics of the micro-food web

Long Non-coding RNA DLEU1 Promotes Cell Proliferation, Invasion, and Confers Cisplatin Resistance in Bladder Cancer by Regulating the miR-99b/HS3ST3B1 Axis

Although accumulating evidence has shown the important function of long non-coding RNAs (lncRNAs) in tumor progression and chemotherapy resistance, the role of lncRNA DLEU1 in regulating proliferation, invasion, and chemoresistance of bladder cancer (BCA) cells remains largely unknown. Here, we found that DLEU1 was upregulated in BLCA tissues and BCA patients with high DLEU1 expression exhibited a shorter survival time. Furthermore, mechanistic analysis and functional assays validated that DLEU1 induced cell proliferation, invasion, and cisplatin resistance of BCA cells by de-repressing the expression of HS3ST3B1 through sponging miR-99b. Low miR-99b and high HS3ST3B1 levels were correlated with worse prognosis in patients with BCA. Ectopic expression of HS3ST3B1 or inhibition of miR-99b reversed DLEU1 knockdown-mediated suppression of cell proliferation, invasion, and cisplatin resistance. Thus, our study revealed a novel role for the DLEU1/miR-99b/HS3ST3B1 axis in regulating proliferation, invasion, and cisplatin resistance of BCA cells

Subgenomic Stability of Progenitor Genomes During Repeated Allotetraploid Origins of the Same Grass Brachypodium hybridum

Both homeologous exchanges and homeologous expression bias are generally found in most allopolyploid species. Whether homeologous exchanges and homeologous expression bias differ between repeated allopolyploid speciation events from the same progenitor species remains unknown. Here, we detected a third independent and recent allotetraploid origin for the model grass Brachypodium hybridum. Our homeologous exchange with replacement analyses indicated the absence of significant homeologous exchanges in any of the three types of wild allotetraploids, supporting the integrity of their progenitor subgenomes and the immediate creation of the amphidiploids. Further homeologous expression bias tests did not uncover significant subgenomic dominance in different tissues and conditions of the allotetraploids. This suggests a balanced expression of homeologs under similar or dissimilar ecological conditions in their natural habitats. We observed that the density of transposons around genes was not associated with the initial establishment of subgenome dominance; rather, this feature is inherited from the progenitor genome. We found that drought response genes were highly induced in the two subgenomes, likely contributing to the local adaptation of this species to arid habitats in the third allotetraploid event. These findings provide evidence for the consistency of subgenomic stability of parental genomes across multiple allopolyploidization events that led to the same species at different periods. Our study emphasizes the importance of selecting closely related progenitor species genomes to accurately assess homeologous exchange with replacement in allopolyploids, thereby avoiding the detection of false homeologous exchanges when using less related progenitor species genomes

Structural boundary and deep contact relationship between the Yangtze and Cathaysia Blocks from crustal thickness gradients

The deep boundary and contact relationship between the Yangtze and Cathaysia Blocks (the major tectonic units of the Southern China Block), as well as the tectonic attributes of the Jiangnan Orogenic Belt, have remained unknown or controversial. Using data recorded by 128 portable broadband stations and 96 permanent stations, we obtained high-resolution images of crustal thickness and Poisson’s ratio in the study area. The influences of crustal anisotropy and inclined interface were eliminated by using the newly proposed receiver function H–κ–c stacking method. We then used a gradient analysis method to obtain crustal thickness gradients at the boundary of the Yangtze and Cathaysia Blocks for the first time. Our results reveal that the crustal thickness varies from >38 km in the Qinling–Dabie Orogenic Belt to <30 km east of the Tanlu Fault and Cathaysia Block. Areas with high Poisson’s ratios (>0.27) are concentrated on the flanks of the deep fault zone and the continental margin of the study area; those with low Poisson’s ratios (<0.23) are concentrated in the Jiangnan Orogenic Belt. Large crustal thickness gradients are found beneath the eastern part of the Jiujiang–Shitai buried fault (>5 km/°). Combined with the velocity structure and discontinuity characteristics at different depths, these findings suggest that the Jiujiang–Shitai fault may constitute a deep tectonic boundary dividing the Yangtze and Cathaysia Blocks on the lithospheric scale. Moreover, our results support that the Cathaysia Block subducted northwest-ward toward the southeastern margin of the Yangtze Block in the Neoproterozoic, and that the Jiujiang–Shitai buried fault and Jiangshan–Shaoxing fault are the deep and shallow crustal contact boundaries of the two Blocks, respectively; that is, the Yangtze Block overlaps the Cathaysia Block

Differential expression of heat shock protein 90, 70, 60 in chicken muscles postmortem and its relationship with meat quality

Objective The aim of this study was to investigate the expression of heat shock protein (HSP) 90, 70, and 60 in chicken muscles and their possible relationship with quality traits of meat. Methods The breast muscles from one hundred broiler chickens were analyzed for drip loss and other quality parameters, and the levels of heat shock protein (HSP) 90, 70, and 60 were determined by immunoblots. Results Based on the data, chicken breast muscles were segregated into low (drip loss≤5%), intermediate (5%0.05). Conclusion Results of this study suggests that higher levels of HSP90 and HSP60 may be advantageous for maintenance of cell function and reduction of water loss, and they could act as potential indicator for better water holding capacity of meat

Application of ultra-high pressure hydraulic slotting technology in medium hardness and low permeability coal seam

In order to improve the gas pre-drainage efficiency of medium hard coal seam with high gas and low permeability, the applicable conditions, advantages and disadvantages of hydraulic piercing, hydraulic fracturing and hydraulic cutting seam anti-permeability technology were discussed. Based on the principle of ultra-high pressure hydraulic slotting technology, a kind of ultra-high pressure hydraulic slotting device for through-layer drilling is developed, it is mainly composed of diamond hydraulic slotting bit, shallow spiral drill pipe, ultra-high pressure rotary joint, ultra-high pressure clean water pump, high-low pressure converter, ultra-high pressure rubber pipe, etc. The water pressure reaches 60−100 MPa, which can realize the integration of drilling and cutting, and is simple and convenient to use. The device was used to carry out field tests in the pre-drainage boreholes of 11-2 coal seam through the floor roadway of 1361(1) haulage gateway in Dingji coal mine. The coal seam gas pressure was 1.43 MPa, the gas content was 8.05 m3/t, and the gas permeability coefficient was 0.013 m2/ (MPa2·d), the coal seam firmness coefficient is 0.79; 1361(1) transportation roadway floor No.11−No.15 drilling area unit length 227 meters, using high-pressure hydraulic slotting anti-permeability measures, 1361(1) transportation roadway floor No.6—No.10 drilling area unit length 213 meters, anti-permeability measures of low-pressure water punching in coal mine. The results show that the average single-knife slitting time of ultra-high pressure hydraulic slitting drilling is 10.7 min, the single-knife coal output is 0.31 t, the equivalent slitting radius is 1.38 m, the slitting density of the coal hole section is 1 knife/m, and the average cutting rate per hole is The average gas drainage concentration of ultra-high pressure hydraulic slotted holes is 56.97%, which is 2.37 times that of low-pressure punching; The time to reach the standard is about 23 days, which is 74.4% and 54.9% shorter than that of ordinary drilling and hydraulic punching technology respectively. Compared with ordinary drilling and hydraulic punching technology, ultra-high pressure hydraulic slotting technology is more ideal for gas drainage in low permeability medium-hard coal seams