L'Écho : grand quotidien d'information du Centre Ouest

29 décembre 19141914/12/29 (A43).Appartient à l’ensemble documentaire : PoitouCh

L'Écho : grand quotidien d'information du Centre Ouest

12 décembre 19371937/12/12 (A66)-1937/12/13.Appartient à l’ensemble documentaire : PoitouCh

miRNAs with down-regulated expression (>10-fold) between LPS-injected female and male samples.

<p>* indicates passenger strand, miR-5p or miR-3p means location of the mature miRNA at the 5p or 3p arm of its precursor.</p><p>miRNAs with >15-fold change are underlined. miRNA</p

Novel miRNAs identified in the four samples.

<p>^a: Novel miRNA name in the LPS-injected female samples;</p><p>^b: novel miRNA also in the PBS-injected female samples;</p><p>^c: novel miRNA also in the LPS-injected male samples;</p><p>^d: novel miRNA also in the PBS-injected male samples;</p><p>^e: location of mature miRNA at the 5p or 3p arm of its precursor; N: no existence; miRNA</p><p>* means passenger strand.</p><p>Novel miRNAs identified in the four samples.</p

Additional file 1: of Caspase-1 participates in apoptosis of salivary glands in Rhipicephalus haemaphysaloides

Annexin V-PI raw data file. (DOCX 1280 kb

Lipopolysaccharide-Induced Differential Expression of miRNAs in Male and Female <i>Rhipicephalus haemaphysaloides</i> Ticks

<div><p>Lipopolysaccharide (LPS) stimulates the innate immune response in arthropods. In tick vectors, LPS activates expression of immune genes, including those for antibacterial peptides. miRNAs are 21–24 nt non-coding small RNAs that regulate target mRNAs at the post-transcriptional level. However, our understanding of tick innate immunity is limited to a few cellular immune reactions and some characterized immune molecules. Moreover, there is little information on the regulation of the immune system in ticks by miRNA. Therefore, this study aimed to analyze the differential expression of miRNAs in male and female ticks after LPS injection. LPS was injected into male and female <i>Rhipicephalus haemaphysaloides</i> ticks to stimulate immune response, with phosphate buffered saline (PBS)-injected ticks as negative controls. miRNAs from each group were sequenced and analyzed. In the PBS- and LPS-injected female ticks, 11.46 and 12.82 million reads of 18–30 nt were obtained respectively. There were 13.92 and 15.29 million reads of 18–30 nt obtained in the PBS- and LPS-injected male ticks, respectively. Expression of miRNAs in male ticks was greater than that in female ticks. There were 955 and 984 conserved miRNA families in the PBS- and LPS-injected female ticks, respectively, and correspondingly 1684 and 1552 conserved miRNA families in male ticks. Nine novel miRNAs were detected as common miRNAs in two or more tested samples. There were 37 known miRNAs up-regulated >10-fold and 33 down-regulated >10-fold in LPS-injected female ticks; and correspondingly 52 and 59 miRNAs in male ticks. Differential expression of miRNAs in PBS- and LPS-injected samples supports their involvement in the regulation of innate immunity. These data provide an important resource for more detailed functional analysis of miRNAs in this species.</p></div

miRNAs with up-regulated expression (>11-fold) between LPS-injected female and male samples.

<p>* indicates passenger strand, miR-5p or miR-3p means location of the mature miRNA at the 5p or 3p arm of its precursor.</p><p>miRNAs with >15-fold change are underlined. miRNA</p

Deep sequencing of sRNA profiles in the four samples.

<p>A) Size distributions of sRNA libraries from the four samples. B) Frequencies of read counts for known miRNAs present in the LPS- and PBS-injected female and male samples.</p

miRNAs with up-regulated expression (>10-fold) after LPS induction in male ticks.

<p>* indicates passenger strand, miR-5p or miR-3p means location of the mature miRNA at the 5p or 3p arm of its precursor.</p><p>miRNAs with >15-fold change are underlined. miRNA.</p

Additional file 1: of Differential sialotranscriptomes of unfed and fed Rhipicephalus haemaphysaloides, with particular regard to differentially expressed genes of cysteine proteases

Differentially expressed genes database and GO terms database involved in this paper. (ZIP 4091 kb