Car-following Behavior Analysis of Left-turn Vehicles at Signalized Intersections

In order to enrich the car-following theory of urban signalized intersections, and reveal the car-following characteristics of left turn at signalized intersections, the car-following behavior of left turn at signalized intersections is studied. The car-following data acquisition test which was based on high precision GPS was designed. And the car-following characteristics of left-turning vehicles at signalized intersections with different turning radii were analyzed. Based on which, the influence of radius on the car-following behavior was explained, and the New Full Velocity Difference (NFVD) model was developed. The genetic algorithm was used to calibrate the parameters of the NFVD model. The stability and accuracy of the calibrated model was further analyzed by using field data. The results showed that the average speed of the following car increases with the turning radius of the signalized intersection; the car-following speed which the highest frequency occurs under different turning radii tends to increase with the enlargement of turning radius; the larger the average headway distance between the car-following vehicles, the more intense of the driver’s response to the deceleration of the front vehicle. These findings could be used in traffic simulation and to make engineering decisions

Protective effects of N-acetylcysteine on acetic acid-induced colitis in a porcine model

BACKGROUND: Ulcerative colitis is a chronic inflammatory disease and involves multiple etiological factors. Acetic acid (AA)-induced colitis is a reproducible and simple model, sharing many characteristics with human colitis. N-acetylcysteine (NAC) has been widely used as an antioxidant in vivo and in vitro. NAC can affect several signaling pathways involving in apoptosis, angiogenesis, cell growth and arrest, redox-regulated gene expression, and inflammatory response. Therefore, NAC may not only protect against the direct injurious effects of oxidants, but also beneficially alter inflammatory events in colitis. This study was conducted to investigate whether NAC could alleviate the AA-induced colitis in a porcine model. METHODS: Weaned piglets were used to investigate the effects of NAC on AA-induced colitis. Severity of colitis was evaluated by colon histomorphology measurements, histopathology scores, tissue myeloperoxidase activity, as well as concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon. The protective role of NAC was assessed by measurements of antioxidant status, growth modulator, cell apoptosis, and tight junction proteins. Abundances of caspase-3 and claudin-1 proteins in colonic mucosae were determined by the Western blot method. Epidermal growth factor receptor, amphiregulin, tumor necrosis factor-alpha (TNF-α), and toll-like receptor 4 (TLR4) mRNA levels in colonic mucosae were quantified using the real-time fluorescent quantitative PCR. RESULTS: Compared with the control group, AA treatment increased (P < 0.05) the histopathology scores, intraepithelial lymphocyte (IEL) numbers and density in the colon, myeloperoxidase activity, the concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon, while reducing (P < 0.05) goblet cell numbers and the protein/DNA ratio in the colonic mucosa. These adverse effects of AA were partially ameliorated (P < 0.05) by dietary supplementation with NAC. In addition, NAC prevented the AA-induced increase in caspase-3 protein, while stimulating claudin-1 protein expression in the colonic mucosa. Moreover, NAC enhanced mRNA levels for epidermal growth factor and amphiregulin in the colonic mucosa. CONCLUSION: Dietary supplementation with NAC can alleviate AA-induced colitis in a porcine model through regulating anti-oxidative responses, cell apoptosis, and EGF gene expression

Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation

IntroductionTranscranial Magnetic Stimulation (TMS) is a noninvasive technique that uses pulsed magnetic fields to affect the physiology of the brain and central nervous system. Repetitive TMS (rTMS) has been used to study and treat several neurological conditions, but its complex molecular basis is largely unexplored.MethodsUtilizing three experimental rat models (in vitro, ex vivo, and in vivo) and employing genome-wide microarray analysis, our study reveals the extensive impact of rTMS treatment on gene expression patterns.ResultsThese effects are observed across various stimulation protocols, in diverse tissues, and are influenced by time and age. Notably, rTMS-induced alterations in gene expression span a wide range of biological pathways, such as glutamatergic, GABAergic, and anti-inflammatory pathways, ion channels, myelination, mitochondrial energetics, multiple neuron-and synapse-specific genes.DiscussionThis comprehensive transcriptional analysis induced by rTMS stimulation serves as a foundational characterization for subsequent experimental investigations and the exploration of potential clinical applications

Protective effects of N-acetylcysteine on acetic acid-induced colitis in a porcine model

BACKGROUND: Ulcerative colitis is a chronic inflammatory disease and involves multiple etiological factors. Acetic acid (AA)-induced colitis is a reproducible and simple model, sharing many characteristics with human colitis. N-acetylcysteine (NAC) has been widely used as an antioxidant in vivo and in vitro. NAC can affect several signaling pathways involving in apoptosis, angiogenesis, cell growth and arrest, redox-regulated gene expression, and inflammatory response. Therefore, NAC may not only protect against the direct injurious effects of oxidants, but also beneficially alter inflammatory events in colitis. This study was conducted to investigate whether NAC could alleviate the AA-induced colitis in a porcine model. METHODS: Weaned piglets were used to investigate the effects of NAC on AA-induced colitis. Severity of colitis was evaluated by colon histomorphology measurements, histopathology scores, tissue myeloperoxidase activity, as well as concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon. The protective role of NAC was assessed by measurements of antioxidant status, growth modulator, cell apoptosis, and tight junction proteins. Abundances of caspase-3 and claudin-1 proteins in colonic mucosae were determined by the Western blot method. Epidermal growth factor receptor, amphiregulin, tumor necrosis factor-alpha (TNF-α), and toll-like receptor 4 (TLR4) mRNA levels in colonic mucosae were quantified using the real-time fluorescent quantitative PCR. RESULTS: Compared with the control group, AA treatment increased (P < 0.05) the histopathology scores, intraepithelial lymphocyte (IEL) numbers and density in the colon, myeloperoxidase activity, the concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon, while reducing (P < 0.05) goblet cell numbers and the protein/DNA ratio in the colonic mucosa. These adverse effects of AA were partially ameliorated (P < 0.05) by dietary supplementation with NAC. In addition, NAC prevented the AA-induced increase in caspase-3 protein, while stimulating claudin-1 protein expression in the colonic mucosa. Moreover, NAC enhanced mRNA levels for epidermal growth factor and amphiregulin in the colonic mucosa. CONCLUSION: Dietary supplementation with NAC can alleviate AA-induced colitis in a porcine model through regulating anti-oxidative responses, cell apoptosis, and EGF gene expression

Modeling Car-Following Behaviour of Turning Movements at Intersections with Consideration of Turning Radius

In order to deeply analyze and describe the characteristics of car-following behaviour of turning vehicles at intersections, the features and application conditions of classic car-following models were analyzed firstly. And then, through analysing the relationship between the maximum velocity of car-following vehicles and the turning radius of intersection, the differences in key variables between turning and straight car-following behaviour were identified. On the basis of Optimal Velocity (OV) model, a Turning Optimal Velocity (TOV) car-following model with consideration of turning radius and sideway force coefficient at intersections was developed. PreScan simulation was employed to build the scene of turning car-following process at an intersection. Based on linear stability analysis, the stability conditions of the TOV model were derived. And it was found that (1) the turning radius has a significant effect on the car-following behaviour of turning vehicles at intersections; (2) with the increase of the distance between vehicles, the driver’s response sensitivity coefficient increases and then decreases and reaches the maximum value when the distance reaches the minimum safe distance; (3) with the increase of turning radius, the stability of the car-following fleet tends to decrease, and it is more likely to become a stop-and-go traffic flow. In addition, the numerical simulation results indicate that the TOV model can describe the car-following behaviour of turning vehicles more accurately with consideration of turning radius. The findings of this study can be used in the development of microscopic traffic simulation software and for improving traffic safety at intersections

Assessing the Variability of Heavy Metal Concentrations in Liquid-Solid Two-Phase and Related Environmental Risks in the Weihe River of Shaanxi Province, China

Accurate estimation of the variability of heavy metals in river water and the hyporheic zone is crucial for pollution control and environmental management. The biotoxicities and potential ecological risks of heavy metals (Cu, Zn, Pb, Cd) in a solid-liquid two-phase system were estimated using the Geo-accumulation Index, Potential Ecological Risk Assessment and Quality Standard Index methods in the Weihe River of Shaanxi Province, China. Water and sediment samples were collected from five study sites during spring, summer and winter, 2013. The dominant species in the streambed sediments were chironomids and flutter earthworm, whose bioturbation mainly ranged from 0 to 20 cm. The concentrations of heavy metals in surface water and pore water varied obviously in spring and summer. The degrees of concentration of Cu and Cd in spring and summer were higher than the U.S. water quality Criteria Maximum Concentrations. Furthermore, the biotoxicities of Pb and Zn demonstrated season-spatial variations. The concentrations of Cu, Zn, Pb and Cd in spring and winter were significantly higher than those in summer, and the pollution levels also varied obviously in different layers of the sediments. Moreover, the pollution level of Cd was the most serious, as estimated by all three assessment methods