A Comparative Study on the Biological Characteristics of Human Adipose-Derived Stem Cells from Lipectomy and Liposuction - Fig 13

<p><b>The LDH levels of ADSCs treated with different concertations of lidocaine (A) and adrenaline (B)</b> (n = 3, * p<0.05).</p

Cell viability of ADSCs from lipectomy and liposuction detected by Trypan blue staining (n = 3, * p<0.05).

<p>Cell viability of ADSCs from lipectomy and liposuction detected by Trypan blue staining (n = 3, * p<0.05).</p

Cell migration of ADSCs as determined by wound scratch assays.

<p>(A) Quantification of the “wound gap” distance between the front lines of migrating cells. (B) Images taken at 0, 6, 12, 24, and 48 hours to visualize the wound gap (n = 3, * p<0.05).</p

Cell attachment rate of ADSCs from lipectomy and liposuction (n = 3, * p<0.05).

<p>Cell attachment rate of ADSCs from lipectomy and liposuction (n = 3, * p<0.05).</p

The apoptosis and necrosis of ADSCs from lipectomy and liposuction detected by flow cytometry analysis (n = 3, * p<0.05).

<p>The apoptosis and necrosis of ADSCs from lipectomy and liposuction detected by flow cytometry analysis (n = 3, * p<0.05).</p

Cell confluence time of ADSCs from lipectomy and liposuction (n = 3,* p<0.05).

<p>Cell confluence time of ADSCs from lipectomy and liposuction (n = 3,* p<0.05).</p

Identification of ADSCs (positive markers: CD73, CD90 and CD105; negative marker: CD34 and CD45) by flow cytometry analysis.

<p>Identification of ADSCs (positive markers: CD73, CD90 and CD105; negative marker: CD34 and CD45) by flow cytometry analysis.</p

Adipogenic differentiation of ADSCs.

<p>(A) Adipose differentiation of ADSCs evaluated by Oil red O staining. (B) Quantitative analysis of the intracellular Oil red O by spectrophotometry (n = 3, * p<0.05).</p

The proliferation of ADSCs, treated with different concertation of lidocaine and adrenaline, detected by CCK-8 assays.

<p>The proliferation of ADSCs, treated with different concertation of lidocaine and adrenaline, detected by CCK-8 assays.</p