Prognosis comparison between small cell carcinoma of ovary and high-grade serous ovarian cancer: A retrospective observational cohort study

BackgroundSmall cell carcinoma of ovary (SCCO) is a rare and aggressive cancer primarily reported in the form of case reports. Due to limited epidemiological and prognostic analyses based on large populations, SCCO has varied considerably without prognostic models and a recognized first-line treatment strategy. The study aimed to compare the clinical characteristics, treatment methods, and prognosis of SCCO and high-grade serous ovarian cancer (HGSOC), the most prevalent subtype of ovarian cancer, in a large sample and develop a predictive model for these two subtypes.MethodsData from the Surveillance, Epidemiology, and End Results program were analyzed for patients with SCCO or HGSOC from 2000 to 2017. Clinical, demographic, and treatment characteristics were compared between the two groups. Propensity-score matching, Cox risk regression analysis, and Kaplan-Meier survival curves were used to assess the data. Finally, a nomogram was developed to predict the patient survival time.ResultsA total of 32,185 women, including 31,979 (99.4%) diagnosed with HGSOC and 206 (0.6%) diagnosed with SCCO, were identified. Age ≤ 51 years, single, median house income less than $70,000, early stage, and unilateral disease were more common characteristics of patients with SCCO than those with HGSOC. Patients with SCCO were more likely to receive radiotherapy (6.8% vs. 0.8%, p <0.001) and have tumors ≥ 141 mm (38.3% vs. 9.7%, p <0.001) than patients with HGSOC. The independent risk factors for SCCO patients included older age at diagnosis, advanced stage, surgery, radiotherapy, chemotherapy, larger tumor size, and bilateral tumor. Overall and cancer-specific survival rates were significantly lower for SCCO than more malignant HGSOC. Prognostic models and nomograms had been constructed to predict the individual survival rates of patients with SCCO and HGSOC.ConclusionPatients with SCCO presented with the early-stage disease more frequently than patients with HGSOC and had decreased overall and cancer-specific survival rates

Spermidine endows macrophages anti-inflammatory properties by inducing mitochondrial superoxide-dependent AMPK activation, Hif-1α upregulation and autophagy.

Distinct metabolic programs, either energy-consuming anabolism or energy-generating catabolism, were required for different biological functions. Macrophages can adopt different immune phenotypes in response to various cues and exhibit anti- or pro-inflammatory properties relying on catabolic pathways associated with oxidative phosphorylation (OXPHOS) or glycolysis. Spermidine, a natural polyamine, has been reported to regulate inflammation through inducing anti-inflammatory (M2) macrophages. However, the underlying mechanisms remain elusive. We show here that the M2-polarization induced by spermidine is mediated by mitochondrial reactive oxygen species (mtROS). The levels of mitochondrial superoxide and H2O2 were markedly elevated by spermidine. Mechanistically, mtROS were found to activate AMP-activated protein kinase (AMPK), which in turn enhanced mitochondrial function. Furthermore, hypoxia-inducible factor-1α (Hif-1α) was upregulated by the AMPK activation and mtROS and was required for the expression of anti-inflammatory genes and induction of autophagy. Consistent with previous report that autophagy is required for the M2 polarization, we found that the M2 polarization induced by spermidine was also mediated by increased autophagy. The macrophages treated with spermidine in vitro were found to ameliorate Dextran Sulfate Sodium (DSS)-induced inflammatory bowel disease (IBD) in mice. Thus, spermidine can elicit an anti-inflammatory program driven by mtROS-dependent AMPK activation, Hif-1α stabilization and autophagy induction in macrophages. Our studies revealed a critical role of mtROS in shaping macrophages into M2-like phenotype and provided novel information for management of inflammatory disease by spermidine

Association between H-RAS T81C genetic polymorphism and gastrointestinal cancer risk: A population based case-control study in China

<p>Abstract</p> <p>Background</p> <p>Gastrointestinal cancer, such as gastric, colon and rectal cancer, is a major medical and economic burden worldwide. However, the exact mechanism of gastrointestinal cancer development still remains unclear. <it>RAS </it>genes have been elucidated as major participants in the development and progression of a series of human tumours and the single nucleotide polymorphism at <it>H-RAS </it>cDNA position 81 was demonstrated to contribute to the risks of bladder, oral and thyroid carcinoma. Therefore, we hypothesized that this polymorphisms in <it>H-RAS </it>could influence susceptibility to gastrointestinal cancer as well, and we conducted this study to test the hypothesis in Chinese population.</p> <p>Methods</p> <p>A population based case-control study, including 296 cases with gastrointestinal cancer and 448 healthy controls selected from a Chinese population was conducted. <it>H-RAS </it>T81C polymorphism was genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) assay.</p> <p>Results</p> <p>In the healthy controls, the TT, TC and CC genotypes frequencies of <it>H-RAS </it>T81C polymorphism, were 79.24%, 19.87% and 0.89%, respectively, and the C allele frequency was 10.83%. Compared with TT genotype, the TC genotype was significantly associated with an increased risk of gastric cancer (adjusted OR = 3.67, 95%CI = 2.21–6.08), while the CC genotype showed an increased risk as well (adjusted OR = 3.29, 95%CI = 0.54–19.86), but it was not statistically significant. In contrast, the frequency of TC genotype was not significantly increased in colon cancer and rectal cancer patients. Further analysis was performed by combining TC and CC genotypes compared against TT genotype. As a result, a statistically significant risk with adjusted OR of 3.65 (95%CI, 2.22–6.00) was found in gastric cancer, while no significant association of <it>H-RAS </it>T81C polymorphism with colon cancer and rectal cancer was observed.</p> <p>Conclusion</p> <p>These findings indicate, for the first time, that there is an <it>H-RAS </it>T81C polymorphism existing in Chinese population, and this SNP might be a low penetrance gene predisposition factor for gastric cancer.</p

Optimal Layout of Electric Vehicle Charging Station Locations Considering Dynamic Charging Demand

This paper proposes an optimization method for electric vehicle charging station locations considering dynamic charging demand. Firstly, the driving characteristics and charging characteristics of the electric vehicle are obtained based on the driving trajectory of the electric vehicle, and the charging demand is predicted using a Monte Carlo simulation. Then a mathematical model with the goal of minimizing the overall cost is constructed, and the impact on carbon emissions is considered in the model. In order to better solve the location model, an improved whale optimization algorithm based on a hybrid strategy is proposed. Finally, the location problem of Shenzhen electric taxi charging stations is analyzed as an example. The results show that when the number of charging stations is set to 19, the comprehensive cost is the smallest and the energy saving and emission reduction effect is good. The improved whale optimization algorithm also has higher solution accuracy and convergence speed than other classical algorithms

The NF-κB modulated miR-194-5p/IGF1R/PPFIBP axis is crucial for the tumorigenesis of ovarian cancer

miRNAs are involved in the tumorigenesis of various malignancies. In the current study, we found that miR-194-5p expression is downregulated in ovarian cancer tissues, and downregulation of miR-194-5p expression promotes proliferation, invasion and migration of human ovarian cancer cells in vitro and ovarian tumor growth in nude mice. We further found that IGF1R and PPFIBP are targets of miR-194-5p, and downregulation of miR-194-5p expression increases IGF1R and PPFIBP expression, resulting in increased proliferation, invasion and migration of ovarian cancer cells. Moreover, we showed that NF-κB can bind to the promoter region of miR-194-5p, and negatively regulate the expression of miR-194-5p in ovarian cancer cells. Taken together, our results suggested a NF-κB modulated miR-194-5p/IGF1R/ PPFIBP axis that is crucial for the tumorigenesis of ovarian cancer, which provides a new insight into the development of ovarian cancer

Long-Term (2017&ndash;2020) Aerosol Optical Depth Observations in Hohhot City in Mongolian Plateau and the Impacts from Different Types of Aerosol

Aerosol optical depth (AOD) measurements for 2017&ndash;2020 in urban Hohhot of the Mongolian plateau, a transition zone between the depopulated zone and East Asian urban agglomeration, were analyzed for the first time. Results show that annual AOD500 and &Aring;ngstr&ouml;m exponent &alpha;440-675 were 0.36 &plusmn; 0.09 and 1.11 &plusmn; 0.16 (2017), 0.41 &plusmn; 0.12 and 0.90 &plusmn; 0.28 (2018), 0.38 &plusmn; 0.09 and 1.13 &plusmn; 0.24 (2019), 0.38 &plusmn; 0.12 and 1.17 &plusmn; 0.22 (2020), respectively, representing a slightly polluted level with a mixed type of coarse dust aerosol and a fine urban/industrial aerosol. Throughout the year, depopulated-zone continental air flows predominated in Hohhot (i.e., NW-quadrant wind), accounting for 82.12% (spring), 74.54% (summer), 63.61% (autumn), and 100% (winter). The clean and strong NW-quadrant air flows induced by the south movement of a Siberian anticyclone resulted in a low 500-nm AOD of 0.30 &plusmn; 0.29, 0.20 &plusmn; 0.15, 0.24 &plusmn; 0.29, and 0.13 &plusmn; 0.08 from spring to winter. Meanwhile, the local emissions from Hohhot city, as well as anthropogenic urban/industrial aerosols transported by southern and western air masses, originating from southern urban agglomeration and western industrial cities (Baotou, Wuhai, etc.), contributed to the highest aerosol loading, with significant transformation rates of the secondary aerosols Sulfate-Nitrate-Ammonium (SNA) of 47.45%, 57.39%, 49.88%, and 45.16&ndash;47.36% in PM2.5 for each season. The extinction fraction of fine aerosols under these anthropogenic trajectories can be as high as 80%, and the largest fine aerosol size was around 0.2&ndash;0.25 &mu;m. Dust aerosols were suspending in urban Hohhot all year, although at different levels for different seasons, and the extinction fraction of dust aerosol during sandstorms was generally higher than 70%

Multilevel Validation of Doppler Wind Lidar by the 325 m Meteorological Tower in the Planetary Boundary Layer of Beijing

The high-frequency monitoring of three-dimensional wind fields is crucial in planetary boundary layer meteorology. Doppler wind lidar and meteorological towers are the most important instruments for site observations of three-dimensional wind fields. This study systematically investigated and compared the performances of three wind measurement instruments: A Doppler wind lidar (Windcube 100s), cup anemometer/wind vane and sonic wind anemometer mounted on the 325 m meteorological tower in the polluted urban city of Beijing. The horizontal wind speed measurements of the Doppler wind lidar closely matched those of the cup anemometer and the sonic wind anemometer with high coefficients of determination (R2: 0.79&ndash;0.96 and 0.90&ndash;0.97, respectively). Moreover, the results also showed good agreement between the three measurements of the prevailing horizontal wind direction. Conversely, there were weak correlations between the vertical wind speeds of the Doppler wind lidar and sonic wind anemometer with low coefficients of determination (R2: 0.30&ndash;0.46). With increasing temporal scale, the consistency in the vertical wind increased. In addition, the Doppler wind lidar seemed to correlate better with the sonic wind anemometer at heights exceeding 300 m (R2: 0.48&ndash;0.77). Note that there was a remarkable difference between the Doppler wind lidar and 325 m meteorological tower observations as the aerosol concentrations changed rapidly. Different wind measurement instruments have unique advantages and are thus irreplaceable. The Doppler wind lidar is better at measuring larger turbulent eddies

The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity of <i>Ceratocystis fimbriata</i>: Insights from Non-Targeted Metabolomics

Ceratocystis fimbriata (C. fimbriata) is a notorious pathogenic fungus that causes sweet potato black rot disease. The APSES transcription factor Swi6 in fungi is located downstream of the cell wall integrity (CWI)-mitogen-activated protein kinase (MAPK) signaling pathway and has been identified to be involved in cell wall integrity and virulence in several filamentous pathogenic fungi. However, the specific mechanisms by which Swi6 regulates the growth and pathogenicity of plant pathogenic fungi remain elusive. In this study, the SWI6 deletion mutants and complemented strains of C. fimbriata were generated. Deletion of Swi6 in C. fimbriata resulted in aberrant growth patterns. Pathogenicity assays on sweet potato storage roots revealed a significant decrease in virulence in the mutant. Non-targeted metabolomic analysis using LC-MS identified a total of 692 potential differentially accumulated metabolites (PDAMs) in the ∆Cfswi6 mutant compared to the wild type, and the results of KEGG enrichment analysis demonstrated significant enrichment of PDAMs within various metabolic pathways, including amino acid metabolism, lipid metabolism, nucleotide metabolism, GPI-anchored protein synthesis, and ABC transporter metabolism. These metabolic pathways were believed to play a crucial role in mediating the growth and pathogenicity of C. fimbriata through the regulation of CWI. Firstly, the deletion of the SWI6 gene led to abnormal amino acid and lipid metabolism, potentially exacerbating energy storage imbalance. Secondly, significant enrichment of metabolites related to GPI-anchored protein biosynthesis implied compromised cell wall integrity. Lastly, disruption of ABC transport protein metabolism may hinder intracellular transmembrane transport. Importantly, this study represents the first investigation into the potential regulatory mechanisms of SWI6 in plant filamentous pathogenic fungi from a metabolic perspective. The findings provide novel insights into the role of SWI6 in the growth and virulence of C. fimbriata, highlighting its potential as a target for controlling this pathogen

Impact of Shifts in Vegetation Phenology on the Carbon Balance of a Semiarid Sagebrush Ecosystem

Dryland ecosystems are critical in regulating the interannual variability of the global terrestrial carbon cycle. The responses of such ecosystems to weather and environmental conditions remain important factors that limit the accurate projections of carbon balance under future climate change. Here, we investigated how shifts in vegetation phenology resulting from changes in weather and environmental conditions influenced ecosystem carbon cycling in one semiarid ecosystem in the Hanford area of central Washington, United States. We examined two years of measurements of the phenology camera, eddy covariance, and soil chamber from an upland semiarid sagebrush ecosystem. Both years had contrasting diel and seasonal patterns of CO2 fluxes, primarily driven by differences in vegetation phenology. The net ecosystem exchange of CO2 (NEE) and evapotranspiration (ET) in 2019 were enlarged by shifted vegetation phenology, as a cold and snow-covered winter and warm and dry winter in 2020 resulted in constrained magnitudes of NEE and ET during the summer months. The annual gross primary productivity (GPP) was much higher in 2019 than in 2020 (−211 vs. −112 gC m−2), whereas ecosystem respiration was comparable in these two years (164 vs. 144 gC m−2). Thus, the annual NEE in 2019 was negative (−47 gC m−2) with the sagebrush ecosystem functioning as a carbon sink, while the positive annual NEE in 2020 indicated that the sagebrush ecosystem functioned as a carbon source. Our results demonstrate that winter snowpack can be a critical driver of annual carbon uptake in semiarid sagebrush ecosystems