Study on failure mechanism and application of double-layer structure floor with large buried depth and high confined water

The first mining of nearly whole rock lower protective layer working face in Pingdingshan coal mining area is used to liberate the Ji group coal resources of its upper threatened by the gas outburst. The mining of the rock layer at a depth of nearly 1000 meters is bound to increase the depth of the floor damage. Once the L5 weak water-rich aquifer in the aquifuge is disturbed, the indirect recharge channel of the cold ash water is formed, which affects the safety and stability of the rock floor. Firstly, the theoretical model of plastic slip line of double-layer structure floor is established, and the analytical solution of maximum failure depth of double-layer floor under three working conditions is derived. Then through the self-designed similar simulation experimental platform of pore water pressure (spring) and stratum effective stress (jack), the deformation form and failure characteristics of stope roof and floor are simulated and analyzed based on digital image correlation technology. Finally, the borehole strain measurement method was used to carry out on-site monitoring of floor fracture development morphology in Ji15-31040 nearly whole rock working face of Pingdingshan No.12 Coal Mine. The results show that the maximum failure depth of Ji15-31040 nearly whole rock working face floor is 16.59 m by using the plastic slip line theory of double-layer structure floor. The similar simulation experiment reveals that the floor failure is concentrated at both ends of the open-off cut and the working face, with obvious lagging failure characteristics. The maximum failure depth is 17.8 m. After the working face advances 159.9 m into full mining, the floor stress gradually recovers. The field measurement results show that the floor rock mass has a compression-shear slip failure at 7.9 m in front of the working face. The floor before and after the working face is pushed through the borehole shows compression-shear and tension-shear failure, respectively. The maximum failure depth of the floor is between 16.5 m and 18 m. The results of field measurement are in good agreement with theoretical calculation and similar simulation test

Down-regulation of P450 genes enhances susceptibility to indoxacarb and alters physiology and development of fall armyworm, Spodoptera frugipreda (Lepidoptera: Noctuidae)

The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), is a pest of many important crops globally. Effective control is challenging, with the pest exhibiting resistance to different synthetic pesticides across various groups. However, the mechanisms employed by resistant insects for overexpression of relevant detoxification genes remain unclear. The activity of detoxification enzymes was investigated in this study. Additionally, using RNA interference (RNAi), a functional analysis was completed of two P450s genes in an indoxacarb resistant population of fall armyworms. Elevated resistance levels (resistance ratio = 31.37-fold) in indoxacarb-selected populations of FAW were observed after 14 generations. The qRT-PCR showed higher expression of two cytochrome P450 genes, CYP321A7 and CYP6AE43, in this selected population compared to the control population. RNAi was applied to knock down the P450 dsCYP321A7 and dsCYP6AE43 genes in the FAW larvae. Droplet feeding of the dsRNAs (CYP321A7 and CYP6AE43) via an artificial diet significantly increased mortality rates in the indoxacarb treated population. A shorter larval developmental time of FAW was detected in all dsRNAs-fed larvae. Correspondingly, larval mass was reduced by dsRNAs in indoxacarb resistant populations of fall armyworm. Larval feeding assays demonstrate that dsRNAs targeting, specifically of CYP321A7 and CYP6AE43 enzymes, could be a beneficial technique in the management of indoxacarb resistant populations. Further study on the potential use of dsRNA and its application should be conducted in efforts to counter the development of resistance in FAW against various insecticides in the field

Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprda

The fall armyworm (FAW) Spodoptera frugiperda is a destructive and polyphagous pest of many essential food crops including maize and rice. The FAW is hard to manage, control, or eradicate, due to its polyphagous nature and voracity of feeding. Here, we report the characterization and functional analysis of the detoxification gene CYP302A1 and how S. frugieprda larvae use a detoxification mechanism to adapt host plants. Results demonstrated that CYP302A1 expression levels were much higher in midgut tissue and the older S. frugiperda larvae. Our current studies revealed the enhanced P450 activity in the midguts of S. frugiperda larvae after exposure to rice plants as compared to corn plants and an artificial diet. Furthermore, higher mortality was observed in PBO treated larvae followed by the exposure of rice plants as compared to the corn plant. The dsRNA-fed larvae showed downregulation of CYP302A1 gene in the midgut. At the same time, higher mortality, reduced larval weight and shorter developmental time was observed in the dsRNA-fed larvae followed by the exposure of rice plant as compared to the corn plant and DEPC-water treated plants as a control. These results concluded that the inducible P450 enzyme system and related genes could provide herbivores with an ecological opportunity to adapt to diverse host plants by utilizing secondary compounds present in their host plants

The Commonly Used Bactericide Bismerthiazol Promotes Rice Defenses against Herbivores

Chemical elicitors that enhance plant resistance to pathogens have been extensively studied, however, chemical elicitors that induce plant defenses against insect pests have received little attention. Here, we found that the exogenous application of a commonly used bactericide, bismerthiazol, on rice induced the biosynthesis of constitutive and/or elicited jasmonic acid (JA), jasmonoyl-isoleucine conjugate (JA-Ile), ethylene and H2O2 but not salicylic acid. These activated signaling pathways altered the volatile profile of rice plants. White-backed planthopper (WBPH, Sogatella furcifera) nymphs and gravid females showed a preference for feeding and/or oviposition on control plants: survival rates were better and more eggs were laid than on bismerthiazol-treated plants. Moreover, bismerthiazol treatment also increased both the parasitism rate of WBPH eggs laid on plants in the field by Anagrus nilaparvatae, and also the resistance of rice to the brown planthopper (BPH) Nilaparvata lugens and the striped stem borer (SSB) Chilo suppressalis. These findings suggest that the bactericide bismerthiazol can induce the direct and/or indirect resistance of rice to multiple insect pests, and so can be used as a broad-spectrum chemical elicitor

Silencing an E3 Ubiquitin Ligase Gene <i>OsJMJ715</i> Enhances the Resistance of Rice to a Piercing-Sucking Herbivore by Activating ABA and JA Signaling Pathways

The RING-type E3 ubiquitin ligases play an important role in plant growth, development, and defense responses to abiotic stresses and pathogens. However, their roles in the resistance of plants to herbivorous insects remain largely unknown. In this study, we isolated the rice gene OsJMJ715, which encodes a RING-domain containing protein, and investigated its role in rice resistance to brown planthopper (BPH, Nilaparvata lugens). OsJMJ715 is a nucleus-localized E3 ligase whose mRNA levels were upregulated by the infestation of gravid BPH females, mechanical wounding, and treatment with JA or ABA. Silencing OsJMJ715 enhanced BPH-elicited levels of ABA, JA, and JA-Ile as well as the amount of callose deposition in plants, which in turn increased the resistance of rice to BPH by reducing the feeding of BPH and the hatching rate of BPH eggs. These findings suggest that OsJMJ715 negative regulates the BPH-induced biosynthesis of ABA, JA, and JA-Ile and that BPH benefits by enhancing the expression of OsJMJ715

A Fusion Feature Extraction Method Using EEMD and Correlation Coefficient Analysis for Bearing Fault Diagnosis

Acceleration sensors are frequently applied to collect vibration signals for bearing fault diagnosis. To fully use these vibration signals of multi-sensors, this paper proposes a new approach to fuse multi-sensor information for bearing fault diagnosis by using ensemble empirical mode decomposition (EEMD), correlation coefficient analysis, and support vector machine (SVM). First, EEMD is applied to decompose the vibration signal into a set of intrinsic mode functions (IMFs), and a correlation coefficient ratio factor (CCRF) is defined to select sensitive IMFs to reconstruct new vibration signals for further feature fusion analysis. Second, an original feature space is constructed from the reconstructed signal. Afterwards, weights are assigned by correlation coefficients among the vibration signals of the considered multi-sensors, and the so-called fused features are extracted by the obtained weights and original feature space. Finally, a trained SVM is employed as the classifier for bearing fault diagnosis. The diagnosis results of the original vibration signals, the first IMF, the proposed reconstruction signal, and the proposed method are 73.33%, 74.17%, 95.83% and 100%, respectively. Therefore, the experiments show that the proposed method has the highest diagnostic accuracy, and it can be regarded as a new way to improve diagnosis results for bearings

Particle Flow Analysis on Mechanical Characteristics of Rock with Two Pre-Existing Fissures

Many research results show that under any stress state the rock mass is most likely to crack, swell, bifurcate, and infiltrate from the fissure tip, resulting in rock engineering instability and failure. In order to study the influence of double fissure angles on rock mechanical characteristics, five rock numerical models with different fissure angles were established by numerical simulation software. Uniaxial compression tests were carried out, and the variation characteristics of rock stress, strain, failure, microcrack, and acoustic emission (AE) were recorded. The test results show that: With increases in the fissure angles, the elastic modulus of rock increased, while the peak strength decreased first and then increased. The number of microcracks in rock was greater at 15° and 75° than at other angles. The microcracks in rock were mainly tensile cracks, and relatively few were shear cracks. The angles of microcracks were mostly concentrated between 0 and 180°, most of which were between 60 and 110°. The failure of rock was relatively light when the fissure angle was15° or 75°, but it produced more and smaller fragments, and the failure was the most serious when the fissure angle was 30°. The angles of the fissures affected the maximum number of AE events, the strain values for the initial AE event, and the maximal AE event. This research can provide some reference for disasters caused by rocks with pre-existing fissures

Particle Flow Analysis on Mechanical Characteristics of Rock with Two Pre-Existing Fissures

Many research results show that under any stress state the rock mass is most likely to crack, swell, bifurcate, and infiltrate from the fissure tip, resulting in rock engineering instability and failure. In order to study the influence of double fissure angles on rock mechanical characteristics, five rock numerical models with different fissure angles were established by numerical simulation software. Uniaxial compression tests were carried out, and the variation characteristics of rock stress, strain, failure, microcrack, and acoustic emission (AE) were recorded. The test results show that: With increases in the fissure angles, the elastic modulus of rock increased, while the peak strength decreased first and then increased. The number of microcracks in rock was greater at 15&deg; and 75&deg; than at other angles. The microcracks in rock were mainly tensile cracks, and relatively few were shear cracks. The angles of microcracks were mostly concentrated between 0 and 180&deg;, most of which were between 60 and 110&deg;. The failure of rock was relatively light when the fissure angle was15&deg; or 75&deg;, but it produced more and smaller fragments, and the failure was the most serious when the fissure angle was 30&deg;. The angles of the fissures affected the maximum number of AE events, the strain values for the initial AE event, and the maximal AE event. This research can provide some reference for disasters caused by rocks with pre-existing fissures

Silencing OsHI-LOX makes rice more susceptible to chewing herbivores, but enhances resistance to a phloem feeder

The jasmonic acid (JA) pathway plays a central role in plant defense responses against insects. Some phloem-feeding insects also induce the salicylic acid (SA) pathway, thereby suppressing the plant’s JA response. These phenomena have been well studied in dicotyledonous plants, but little is known about them in monocotyledons. We cloned a chloroplast-localized type 2 13-lipoxygenase gene of rice, OsHI-LOX, whose transcripts were up-regulated in response to feeding by the rice striped stem borer (SSB) Chilo suppressalis and the rice brown planthopper (BPH) Niaparvata lugens, as well as by mechanical wounding and treatment with JA. Antisense expression of OsHI-LOX (as-lox) reduced SSB- or BPH-induced JA and trypsin protease inhibitor (TrypPI) levels, improved the larval performance of SBB as well as that of the rice leaf folder (LF) Cnaphalocrocis medinalis, and increased the damage caused by SSB and LF larvae. In contrast, BPH, a phloem-feeding herbivore, showed a preference for settling and ovipositing on WT plants, on which they consumed more and survived better than on as-lox plants. The enhanced resistance of as-lox plants to BPH infestation correlated with higher levels of BPH-induced H2O2 and SA, as well as with increased hypersensitive response-like cell death. These results imply that OsHI-LOX is involved in herbivore-induced JA biosynthesis, and plays contrasting roles in controlling rice resistance to chewing and phloem-feeding herbivores. The observation that suppression of JA activity results in increased resistance to an insect indicates that revision of the generalized plant defense models in monocotyledons is required, and may help develop novel strategies to protect rice against insect pests

<i>OsRCI-1</i>-Mediated GLVs Enhance Rice Resistance to Brown Planthoppers

Green leaf volatiles (GLVs) play pivotal roles in plant anti-herbivore defense. This study investigated whether the rice 13-lipoxygense gene OsRCI-1 is involved in GLV production and plant defense in rice. The overexpression of OsRCI-1 (oeRCI lines) in rice resulted in increased wound-induced levels of two prominent GLVs, cis-3-hexen-1-ol and cis-3-hexenal. In a previous study, we found that the overexpression of OsRCI-1 reduced the colonization by the rice brown planthopper (BPH, Nilaparvata lugens) but increased the attractiveness to the egg parasitoid Anagrus nilaparvatae compared to wild-type (WT) plants. This study found that when cis-3-hexen-1-ol, but not cis-3-hexenal, was added to WT plants, it could change the BPH’s colonization preference, i.e., more BPHs preferred to colonize the oeRCI lines. The exogenous application of cis-3-hexen-1-ol or cis-3-hexenal to BPH-infested WT plants could weaken or overturn the preference of A. nilaparvatae for oeRCI lines. However, field experiments revealed that only cis-3-hexenal was attractive to the parasitoid and increased the parasitism rates of BPH eggs. These results indicate that OsRCI-1 is involved in rice GLV production and therefore modulates both direct and indirect defense in rice