Effect of Selected Agrochemicals on Protozoans and Algae Isolated from Mosquito Larval Habitats in Bamenda

The present study establishes the in-vitro effect of some selected agrochemicals(Glycot, Lamida and Pencozeb) on protozoans and some algae isolated from mosquito larval habitats in Bamenda urban zone, Cameroon. A convenience sampling technique was used in which water samples were randomly collected from 125 mosquito larval habitats from Bamenda urban zone. Out of the 123 samples tested, 41(33.33%) samples were found to be positive. Dinoflagellates predorminated with a frequency of 20(16.26%). One genera of protozoa(chillodenella) pathogenic to mosquito larvae was seen. Hook worm, Faciola, Paramecium and Trichomonas were found to be of medical importance while Euglena, Diatoms and Dinoflagellates were found to be of agricultural importance. All the protozoans and algae tested were susceptible to Lamida and Penncozeb, but resistant to glycot at all concentrations tested. Conclusively, the susceptibility of Chillodenella spp to Lamida and Penncozeb suggest their interference with natural biological control for mosquito population and also distortion of the ecosystem. Keywords: Agrochemicals, protozoans and algae , mosquito larval habitat

Baseline Study on the Occurrence of Cryptosporidium Spp from Streams Water, after Torrential Rains in Bamenda, Cameroon

Oocycts of Cryptosporidia are zoonotic infective stages of Cryptosporidia spp that can easily contaminate the environment if not properly disposed of. Torrential rains would usually wash them into the nearby streams and likely be consumed by humans when the water is used. Cryotosporidiosis in people with suppressed immune systems have no cure as the robust oocycst are not destroyed by chlorination. The aim of this study is to determine the presence of Cryptosporia oocycts in stream water after torrential rains 60 samples of stream water were collected immediately after rain storm in June and July 2014, from 5 streams flowing through Bamenda town. 2 samples of 1.5L were collected from each stream, on 6 different days. Cryptosporidia ssp were present in all the streams but there was no significant difference between their occurrence and the stream’s location, Chi square = 2.605, >0.05The streams flowing through Bamenda town are polluted Keywords: storm-water, zoonotic, Cryptosporidiosis, chlorination, oocyst

Disinfection Practices and Knowledge of Hair Care Professional Barbers in the City of Dschang, Cameroon

Background: Disinfection and sterilization are key processes required to ensure the safety of clients when they visit a barber for a haircut. Barbering practices differ across barbershops depending on the knowledge and economic status of the barber. Several studies have reported on disinfection methods and disinfectants used in barbershops but the manner in which these methods are implemented were not revealed as well as the working concentrations of the disinfectants they used. This study aimed to uncover knowledge on decontamination practices and disinfectants used in barbershops in Dschang and also to assess barbers’ knowledge on these practices and disinfectant substances.Methods: A randomized cross-sectional and observational study approach was conducted amongst 57 consented barbers in the city of Dschang. An interview was held with each barber after observing at least a single haircut and a questionnaire filled during the process by the investigator. Collected data was analysed using GraphPad Prism 5, SPSS21 and Microsoft Excel 2013 and the outcome presented as descriptive statistics.Results: Barbers in Dschang employed flaming (53%), UV light exposure (23%) and alcohol treatment (14%) as their main disinfection methods but performed no sterilization (100%). Disinfectants commonly used were alcohol by 96.5% barbers and sodium or calcium hypochlorite (eau de Javel) by 86% users. Knowledge on the use of these disinfectants and decontamination processes was poor amongst barbers with 94.74% alcohol and 86% sodium hypochlorite poor users.Conclusion: Barbers’ awareness of disinfection in their practice was very impressive but their entire decontamination processes were poor and depicting a contamination risk. Keywords: Disinfection, Sterilization, Decontamination, barber, barbering. DOI: 10.7176/JHMN/85-0 Publication date: January 31st 202

Effects of Gladiolus dalenii on the Stress-Induced Behavioral, Neurochemical, and Reproductive Changes in Rats

Gladiolus dalenii is a plant commonly used in many regions of Cameroon as a cure for various diseases like headaches, epilepsy, schizophrenia, and mood disorders. Recent studies have revealed that the aqueous extract of G. dalenii (AEGD) exhibited antidepressant-like properties in rats. Therefore, we hypothesized that the AEGD could protect from the stress-induced behavioral, neurochemical, and reproductive changes in rats. The objective of the present study was to elucidate the effect of the AEGD on behavioral, neurochemical, and reproductive characteristics, using female rats subjected to chronic immobilization stress. The chronic immobilization stress (3 h per day for 28 days) was applied to induce female reproductive and behavioral impairments in rats. The immobilization stress was provoked in rats by putting them separately inside cylindrical restrainers with ventilated doors at ambient temperature. The plant extract was given to rats orally everyday during 28 days, 5 min before induction of stress. On a daily basis, a vaginal smear was made to assess the duration of the different phases of the estrous cycle and at the end of the 28 days of chronic immobilization stress, the rat’s behavior was assessed in the elevated plus maze. They were sacrificed by cervical disruption. The organs were weighed, the ovary histology done, and the biochemical parameters assessed. The findings of this research revealed that G. dalenii increased the entries and the time of open arm exploration in the elevated plus maze. Evaluation of the biochemical parameters levels indicated that there was a significant reduction in the corticosterone, progesterone, and prolactin levels in the G. dalenii aqueous extract treated rats compared to stressed rats whereas the levels of serotonin, triglycerides, adrenaline, cholesterol, glucose estradiol, follicle stimulating hormone and luteinizing hormone were significantly increased in the stressed rats treated with, G. dalenii, diazepam and in co-administration of the plant extract and diazepam treated rats. Moreover stressed rats showed significant changes in estrous cycle phases compared to vehicle control and these changes of the estrous cycle were less in the rats treated with G. dalenii compared to the negative control rats. G. dalenii extract showed antagonizing effects on the stress-induced reproductive, behavioral, and neurochemical changes. These effects could be related to the bioactive molecules and secondary metabolites like alkaloids and flavonoids in the plant

Perspective: Food Environment Research Priorities for Africa-Lessons from the Africa Food Environment Research Network.

Over the last 2 decades, many African countries have undergone dietary and nutrition transitions fueled by globalization, rapid urbanization, and development. These changes have altered African food environments and, subsequently, dietary behaviors, including food acquisition and consumption. Dietary patterns associated with the nutrition transition have contributed to Africa&#8217;s complex burden of malnutrition-obesity and other diet-related noncommunicable diseases (DR-NCDs)-along with persistent food insecurity and undernutrition. Available evidence links unhealthy or obesogenic food environments (including those that market and offer energy-dense, nutrient-poor foods and beverages) with suboptimal diets and associated adverse health outcomes. Elsewhere, governments have responded with policies to improve food environments. However, in Africa, the necessary research and policy action have received insufficient attention. Contextual evidence to motivate, enable, and create supportive food environments in Africa for better population health is urgently needed. In November 2020, the Measurement, Evaluation, Accountability, and Leadership Support for Noncommunicable Diseases Prevention Project (MEALS4NCDs) convened the first Africa Food Environment Research Network Meeting (FERN2020). This 3-d virtual meeting brought researchers from around the world to deliberate on future directions and research priorities related to improving food environments and nutrition across the African continent. The stakeholders shared experiences, best practices, challenges, and opportunities for improving the healthfulness of food environments and related policies in low- and middle-income countries. In this article, we summarize the proceedings and research priorities identified in the meeting to advance the food environment research agenda in Africa, and thus contribute to the promotion of healthier food environments to prevent DR-NCDs, and other forms of&nbsp;malnutrition.</p