In ovo serial skeletal muscle diffusion tractography of the developing chick embryo using DTI: feasibility and correlation with histology

Abstract Background Magnetic resonance imaging is a noninvasive method of evaluating embryonic development. Diffusion tensor imaging (DTI), based on the directional diffusivity of water molecules, is an established method of evaluating tissue structure. Yet embryonic motion degrades the in vivo acquisition of long-duration DTI. We used a dual-cooling technique to avoid motion artifact and aimed to investigate whether DTI can be used to monitor chick embryonic skeletal muscle development in ovo, and to investigate the correlation between quantitative DTI parameters fractional anisotropy (FA) and fiber length and quantitative histologic parameters fiber area percentage (FiberArea%) and limb length. Results From 84 normally developing chick embryos, 5 were randomly chosen each day from incubation days 5 to 18 and scanned using 3.0 Tesla magnetic resonance imaging. A dual-cooling technique is used before and during imaging. Eggs were cracked for making histological specimen after imaging. 3 eggs were serially imaged from days 5 to 18. We show that skeletal muscle fibers can be tracked in hind limb in DTI beginning with incubation day 8. Our data shows a good positive correlation between quantitative DTI and histologic parameters (FA vs FiberArea%: r= 0.943, p\u3c0.0001; Fiber_length vs Limb_length: r=0.974, p\u3c0.0001). The result of tracked fibers in DTI during incubation corresponds to the development of chick embryonic skeletal muscle as reported in the literature. Conclusion Diffusion tensor imaging can provide a noninvasive means of evaluating skeletal muscle development in ovo

A novel multiparametric approach to 3D quantitative MRI of the brain

Magnetic Resonance properties of tissues can be quantified in several respects: relaxation processes, density of imaged nuclei, magnetism of environmental molecules, etc. In this paper, we propose a new comprehensive approach to obtain 3D high resolution quantitative maps of arbitrary body districts, mainly focusing on the brain. The theory presented makes it possible to map longitudinal (R1), pure transverse (R2) and free induction decay (R2 ) rates, along with proton density (PD) and magnetic susceptibility (χ), from a set of fast acquisition sequences in steady-state that are highly insensitive to flow phenomena. A novel denoising scheme is described and applied to the acquired datasets to enhance the signal to noise ratio of the derived maps and an information theory approach compensates for biases from radio frequency (RF) inhomogeneities, if no direct measure of the RF field is available. Finally, the results obtained on sample brain scans of healthy controls and multiple sclerosis patients are presented and discussed

Improved Siderotic Nodule Detection in Cirrhosis with Susceptibility-Weighted Magnetic Resonance Imaging: A Prospective Study

BACKGROUND: Hepatic cirrhosis is a common pathway of progressive liver destruction from multiple causes. Iron uptake can occur within the hepatic parenchyma or within the various nodules that form in a cirrhotic liver, termed siderotic nodules. Siderotic nodule formation has been shown to correlate with inflammatory activity, and while the relationship between siderotic nodule formation and malignancy remains unclear, iron distribution within hepatic nodules has known implications for the detection of hepatocellular carcinoma. We aimed to evaluate the role of abdominal susceptibility-weighted imaging in the detection of siderotic nodules in cirrhotic patients. METHODOLOGY/PRINCIPAL FINDINGS: Forty-six (46) cirrhotic patients with at least one siderotic nodule detected on previous imaging underwent both computed tomography and magnetic resonance imaging (T1-, T2-, T2*-, and susceptibility-weighted imaging) at 3.0 Tesla. Imaging data was independently analyzed by two radiologists. Siderotic nodule count was determined for each modality and imaging sequence. For each magnetic resonance imaging technique, siderotic nodule conspicuity was assessed on a 3 point scale (1 = weak, 2 = moderate, 3 = strong). More nodules were detected by susceptibility weighted imaging (n = 2935) than any other technique, and significantly more than by T2* weighted imaging (n = 1696, p<0.0001). Lesion conspicuity was also highest with susceptibility-weighted imaging, with all nodules found to be moderate (n = 6) or strong (n = 40); a statistically significant difference (p<0.001). CONCLUSIONS: Susceptibility-weighted imaging had the greatest lesion conspicuity and detected the highest number of siderotic nodules suggesting it is the most sensitive imaging technique to detect siderotic nodules in cirrhotic patients

Sciences for The 2.5-meter Wide Field Survey Telescope (WFST)

The Wide Field Survey Telescope (WFST) is a dedicated photometric survey facility under construction jointly by the University of Science and Technology of China and Purple Mountain Observatory. It is equipped with a primary mirror of 2.5m in diameter, an active optical system, and a mosaic CCD camera of 0.73 Gpix on the main focus plane to achieve high-quality imaging over a field of view of 6.5 square degrees. The installation of WFST in the Lenghu observing site is planned to happen in the summer of 2023, and the operation is scheduled to commence within three months afterward. WFST will scan the northern sky in four optical bands (u, g, r, and i) at cadences from hourly/daily to semi-weekly in the deep high-cadence survey (DHS) and the wide field survey (WFS) programs, respectively. WFS reaches a depth of 22.27, 23.32, 22.84, and 22.31 in AB magnitudes in a nominal 30-second exposure in the four bands during a photometric night, respectively, enabling us to search tremendous amount of transients in the low-z universe and systematically investigate the variability of Galactic and extragalactic objects. Intranight 90s exposures as deep as 23 and 24 mag in u and g bands via DHS provide a unique opportunity to facilitate explorations of energetic transients in demand for high sensitivity, including the electromagnetic counterparts of gravitational-wave events detected by the second/third-generation GW detectors, supernovae within a few hours of their explosions, tidal disruption events and luminous fast optical transients even beyond a redshift of 1. Meanwhile, the final 6-year co-added images, anticipated to reach g about 25.5 mag in WFS or even deeper by 1.5 mag in DHS, will be of significant value to general Galactic and extragalactic sciences. The highly uniform legacy surveys of WFST will also serve as an indispensable complement to those of LSST which monitors the southern sky.Comment: 46 pages, submitted to SCMP

Evidence for Diffuse Central Retinal Edema In Vivo in Diabetic Male Sprague Dawley Rats

Background: Investigations into the mechanism of diffuse retinal edema in diabetic subjects have been limited by a lack of animal models and techniques that co-localized retinal thickness and hydration in vivo. In this study we test the hypothesis that a previously reported supernormal central retinal thickness on MRI measured in experimental diabetic retinopathy in vivo represents a persistent and diffuse edema. Methodology/Principal Findings: In diabetic and age-matched control rats, and in rats experiencing dilutional hyponatremia (as a positive edema control), whole central retinal thickness, intraretinal water content and apparent diffusion coefficients (ADC, ‘water mobility’) were measured in vivo using quantitative MRI methods. Glycated hemoglobin and retinal thickness ex vivo (histology) were also measured in control and diabetic groups. In the dilutional hyponatremia model, central retinal thickness and water content were supernormal by quantitative MRI, and intraretinal water mobility profiles changed in a manner consistent with intracellular edema. Groups of diabetic (2, 3, 4, 6, and 9 mo of diabetes), and age-matched controls were then investigated with MRI and all diabetic rats showed supernormal whole central retinal thickness. In a separate study in 4 mo diabetic rats (and controls), MRI retinal thickness and water content metrics were significantly greater than normal, and ADC was subnormal in the outer retina; the increase in retinal thickness was not detected histologically on sections of fixed and dehydrated retinas from these rats

Effect of Pulsed Magnetic Field on Spark Plasma Sintering of Iron-Based Powders

Iron-based powders were sintered by spark plasma sintering coupled with different pulsed magnetic field strength ranging from 0 to 3.93MAm1. The effects of pulsed magnetic field on the sintering behavior of the powders as well as the microstructure and mechanical properties of sintered alloys were investigated. The results showed that the sintering temperature field on the cross section of sample was more uniform via coupling a pulsed magnetic field. The density, hardness and bending strength of the alloy sintered by coupling an appropriate pulsed magnetic field, arose to 7.75 gcm3, 55 HRC and 1235MPa, respectively. There was no remarkable change of sintered density with a further increase of pulsed magnetic field strength, while the hardness and bending strength of sintered alloys adversely decreased. The roles of pulsed magnetic field coupled with electric field are explained to accelerate the diffusion and reaction of alloying elements by raising sintering temperature, facilitate powders rearrangement, intensify sparking among powders, improve the growth of sintering neck and the formation of new sintering neck, and reduce the sintering temperature gradient on cross section. [doi:10.2320/matertrans.M2010057

Safety Evaluation of Crossing Tunnel Engineering: A Case Study

With the growing demand for transportation, there has been a significant increase in road-related projects, leading to potential risks in the safety of existing structures. This paper presents a study on the influence of new tunnel construction on the stability of an adjacent existing tunnel and provides valuable insights for the safety technology evaluation of similar tunnel crossing projects. In order to evaluate the influence of tunnel excavation on adjacent tunnels, the excavation process of a new tunnel near the original tunnel is simulated using the finite element software Midas GTS. This analysis includes the evaluation of static and dynamic responses. The results indicate that the horizontal and vertical displacements caused by the excavation of the railway tunnel are minimal. Furthermore, during the blasting excavation of the railway tunnel, the vibration velocity experienced by the highway tunnel remains below the safe allowable limit. These findings demonstrate that in this project, the influence of blasting excavation for the railway tunnel on the highway tunnel is both safe and manageable

A Unified Binary Neutron Star Merger Magnetar Model for the Chandra X-Ray Transients CDF-S XT1 and XT2

Two bright X-ray transients were reported from the Chandra Deep Field South (CDF-S) archival data, namely CDF-S XT1 and XT2. Whereas the nature of the former is not identified, the latter was suggested as an excellent candidate for a rapidly spinning magnetar born from a binary neutron star (BNS) merger. Here we propose a unified model to interpret both transients within the framework of the BNS merger magnetar model. According to our picture, CDF-S XT2 is observed from the free zone where the magnetar spindown powered X-ray emission escapes freely, whereas CDF-S XT1 originates from the trapped zone where the X-ray emission is initially blocked by the dynamical ejecta and becomes transparent after the ejecta is pushed to a distance where Thomson optical depth drops below unity. We fit the magnetar model to the light curves of both transients and derived consistent parameters for the two events, with magnetic field, initial spin period, and X-ray emission efficiency being (B p = 1016 G, P i = 1.2 ms, η = 0.001) and (B p = 1015.8 G, P i = 4.4 ms, η = 0.001) for XT1 and XT2, respectively. The isotropic equivalent ejecta mass of XT1 is M ej ~ 10−3 M ⊙, while it is not constrained for XT2. Our results suggest that more extreme magnetar parameters are required to have XT1 detected from the trapped zone. The model parameters for both events are generally consistent with those derived from short gamma-ray burst (SGRB) X-ray plateau observations. The host-galaxy properties of both transients are also consistent with those of SGRBs. The event rate densities of both XT1 and XT2 are consistent with that of BNS mergers