The application of daily interruption in the treatment of analgesia and sedation in general ICU

目的  探讨在综合ICU镇痛镇静患者护理方面实施每日唤醒疗法护理的方法和效果。方法  选择在ICU进行持续镇痛镇静治疗>24h的患者共90例，实施每日唤醒和相应护理计划并观察记录。结果  实施每日唤醒后，镇痛镇静药物用量减少，机械通气时间减少，与镇静相关的躁动、谵妄事件减少，住ICU时间缩短。结论  ICU镇痛镇静患者实施每日唤醒可有利于其疾病恢复，减少镇静相关不良事件，值得临床关注，但必须加强病情监测和安全护理。Objective: To explore method and effect of daily interruption therapy in the treatment of analgesia and sedation in general ICU. Methods: 90 patients in ICU, who had been treated with continuous analgesia therapy within 24 hours, were selected and would receive daily interruption therapy and corresponding nursing. Results: Analgesic dosage of sedative drugs was reduced, together with mechanical ventilation time, and delirium events associated with calm restless, ICU stay was shortened after the implement of daily interruption therapy and nursing. Conclusion: The application of daily interruption in general ICU is beneficial to their disease recovery, reduce adverse events related with calmness, worthy of clinical attention, but it is imperative to improve the security of condition monitoring and nursing

The role of Omi/HtrA2 protease in neonatal postasphyxial serum-induced apoptosis in human kidney proximal tubule cells

Omi/HtrA2, a proapoptotic mitochondrial serine protease, is involved in both caspase-dependent and caspaseindependent apoptosis. A growing body of evidence indicates that Omi/HtrA2 plays an important role in the pathogenesis of a variety of ischemia-reperfusion (I/R) injuries. However, the role of Omi/HtrA2 in renal injuries that occur in neonates with asphyxia remains unknown. The present study was designed to investigate whether Omi/HtrA2 plays an important role in the types of renal injuries that are induced by neonatal postasphyxial serum. Human renal proximal tubular cell line (HK-2) cells were used as targets. A 20% serum taken from neonates one day after asphyxia was applied to target cells as an attacking factor. We initially included control and postasphyxial serum-attacked groups and later included a ucf-101 group in the study. In the postasphyxial serum-treated group, cytosolic Omi/HtrA2 and caspase-3 expression in HK-2 cells was significantly higher than in the control group. Moreover, the concentration of cytosolic caspase-3 was found to be markedly decreased in HK-2 cells in the ucf-101 group. Our results suggest both that postasphyxial serum has a potent apoptosis-inducing effect on HK-2 cells and that this effect can be partially blocked by ucf-101. Taken together, our results demonstrate for the first time that postasphyxial serum from neonates results in Omi/HtrA2 translocation from the mitochondria to the cytosol, where it promotes HK-2 cell apoptosis via a protease activity-dependent, caspase-mediated pathway

STUB1 suppresseses tumorigenesis and chemoresistance through antagonizing YAP1 signaling

Yes-associated protein (YAP) is a component of the canonical Hippo signaling pathway that is known to play essential roles in modulating organ size, development, and tumorigenesis. Activation or upregulation of YAP1, which contributes to cancer cell survival and chemoresistance, has been verified in different types of human cancers. However, the molecular mechanism of YAP1 upregulation in cancer is still unclear. Here we report that the E3 ubiquitin ligase STUB1 ubiquitinates and destabilizes YAP1, thereby inhibiting cancer cell survival. Low levels of STUB1 expression were correlated with increased protein levels of YAP1 in human gastric cancer cell lines and patient samples. Moreover, we revealed that STUB1 ubiquitinates YAP1 at the K280 site by K48-linked polyubiquitination, which in turn increases YAP1 turnover and promotes cellular chemosensitivity. Overall, our study establishes YAP1 ubiquitination and degradation mediated by the E3 ligase STUB1 as an important regulatory mechanism in gastric cancer, and provides a rationale for potential therapeutic interventions

Lactulose enhances neuroplasticity to improve cognitive function in early hepatic encephalopathy

Lactulose is known to improve cognitive function in patients with early hepatic encephalopathy; however, the underlying mechanism remains poorly understood. In the present study, we investigated the behavioral and neurochemical effects of lactulose in a rat model of early hepatic encephalopathy induced by carbon tetrachloride. Immunohistochemistry showed that lactulose treatment promoted neurogenesis and increased the number of neurons and astrocytes in the hippocampus. Moreover, lactulose-treated rats showed shorter escape latencies than model rats in the Morris water maze, indicating that lactulose improved the cognitive impairments caused by hepatic encephalopathy. The present findings suggest that lactulose effectively improves cognitive function by enhancing neuroplasticity in a rat model of early hepatic encephalopathy

Proizvodnja novog probiotičkog sira tipa Cheddar, veće ACE inhibicijske aktivnosti i većeg udjela γ-aminomaslačne kiseline, s pomoću Lactobacillus casei Zhang, izolirane iz fermentiranoga mliječnog napitka

Cheddar cheese has been manufactured with Lactobacillus casei Zhang as the dairy starter adjunct. L. casei Zhang had previously been isolated from koumiss collected from Xilin Guole in Inner Mongolia and characterized in detail with regard to their probiotic potential. The addition of L. casei Zhang to Cheddar cheese had no adverse effects on sensory criteria. The cheese made with 0.1, 1 and 2 % of the probiotic strain L. casei Zhang adjuncts contained high levels of the Lactobacillus after 6 months of ripening with final counts of 9.6·10^7, 7.7·10^7 and 1.02·10^8 CFU/g, respectively. In the ripe control cheese, without the addition of probiotic strain L. casei Zhang, the number of Lactobacillus reached 5.7·107 CFU/g. Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) analysis was used to distinguish the added L. casei Zhang from the natural flora of the cheese and to determine whether L. casei Zhang grew in the cheese. ACE-inhibitory activity and γ-aminobutyric acid (GABA) concentrations in the cheese were measured. Compared with control cheese, experimental cheese with 0.1, 1 and 2 % of probiotic strain L. casei Zhang revealed some increase in ACE-inhibitory activity and GABA mass fraction. In the present study, the production of both ACE-inhibitory activity and GABA in the probiotic cheese with the L. casei Zhang adjunct isolated from koumiss has been found for the first time. The results suggest that cheese with the probiotic strain L. casei Zhang showed good potential for application in the management of hypertension.Proizveden je sir tipa Cheddar s pomoću dodane kulture Lactobacillus casei Zhang, prethodno izolirane iz fermentiranoga mliječnog napitka „koumiss“ (Xilin Guole, središnja Mongolija, Kina). Iscrpno su ispitana probiotička svojstva izolirane kulture. Utvrđeno je da dodatak kulture nije bitno promijenio senzorička svojstva sira. Sir pripremljen s 0,1 % probiotičke kulture imao je nakon 6 mjeseci zrenja 9,6·107 CFU/g, sir s 1 % probiotičke kulture 7,7·107 CFU/g, a sir s 2 % probiotičke kulture 1,02·108 CFU/g bakterija roda Lactobacillus. U zrelom je kontrolnom uzorku sira (bez dodatka L. casei Zhang) broj bakterija Lactobacillus bio 5,7·107 CFU/g. Provedbom ERIC-PCR analize razlučena je dodana kultura L. casei Zhang od prirodne mikroflore sira, te utvrđen njezin rast. Također je izmjerena veća ACE inhibicijska aktivnost te veći udio γ-aminomaslačne kiseline u dobivenom siru, u usporedbi s kontrolnim uzorkom. Rezultati pokazuju da se dodatkom probiotičke kulture L. casei Zhang dobiva sir boljih svojstava, čija veća primjena pridonosi liječenju povišenog arterijskog tlaka

Percutaneous closure of a post-traumatic ventricular septal defect with a patent ductus arteriosus occluder

OBJECTIVE: Ventricular septal defects resulting from post-traumatic cardiac injury are very rare. Percutaneous closure has emerged as a method for treating this disorder. We wish to report our experience in three patients who underwent percutaneous closure of a post-traumatic ventricular septal defect with a patent ductus arteriosus occluder. METHODS: We treated three patients with post-traumatic ventricular septal defects caused by stab wounds with knives. After the heart wound was repaired, patient examinations revealed ventricular septal defects with pulmonary/systemic flow ratios (Qp/Qs) of over 1.7. The post-traumatic ventricular septal defects were closed percutaneously with a patent ductus arteriosus occluder (Lifetech Scientific (Shenzhen) Co., LTD, Guangdong, China) utilizing standard techniques. RESULTS: Post-operative transthoracic echocardiography revealed no residual left-to-right shunt and indicated normal ventricular function. In addition, 320-slice computerized tomography showed that the occluder was well placed and exhibited normal morphology. CONCLUSION: Our experiences indicate that closure of a post-traumatic ventricular septal defect using a patent ductus arteriosus occluder is feasible, safe, and effective

4,6-Dimethyl-2-(naphthalen-1-yl)pyrimidine

The asymmetric unit of the title compound, C16H14N2, contains two independent mol­ecules in which the dihedral angles between the pyrimidine and naphthaline rings are 38.20 (5) and 39.35 (5)°. Inter­molecular C—H⋯π contacts and π–π stacking inter­actions [centroid–centroid distances = 3.766 (1) and 3.792 (1) Å] are present in the crystal structure