The detection of NS1 in WNV-infected mice sera by the NS1 antigen-capture ELISA.

<p>The data represent the OD₄₅₀ of the serum samples at 1∶100 dilution. The dashed line represents the cut-off value, which is twice the mean value of the negative control. Each data point represents the mean OD₄₅₀ of duplicate assays. The results were considered positive if a sample yielded an OD₄₅₀ above the cut-off value. The cut-off value was set as twice the mean value of the negative control. Each data point represents the mean OD₄₅₀ of duplicate assays.</p

Evaluation of the sensitivity and specificity of the rWNV-NS1 antigen-capture ELISA.

<p>A, The NS1 standard curve as determined by the purified rWNV-NS1 antigen-capture ELISA. Various concentrations of WNV-NS1 and DENV1-4-NS1 proteins were analysed. BSA was used to establish the baseline. B, The detection of NS1 in WNV-infected cell culture supernatants and other closely related members of the flavivirus family, DENV, JEV, YFV, and TBEV, by the NS1 antigen-capture ELISA. Two-fold serially diluted supernatants from WNV-, four DENV serotype-, JEV-, YFV-, or TBEV-infected cells were subjected to the WNV-NS1 antigen-capture ELISA. Data points represent the mean ± standard deviation from three replicates. The cut-off value was set at twice the average value of the negative controls from three replicates. Positive OD450 values were only observed for the WNV-NS1 protein and WNV-infected cell supernatants.</p

Comparison of the sensitivities of the WNV-NS1 ELISA and real-time RT-PCR for the detection of WNV-infected mice serum samples.

<p>Comparison of the sensitivities of the WNV-NS1 ELISA and real-time RT-PCR for the detection of WNV-infected mice serum samples.</p

The sensitivity of the double antibody sandwich ELISA using different capture and detection MAbs pairs.

<p>The detection of NS1 in culture supernatants of WNV-infected cells by different MAb pairs. The culture supernatants of WNV-infected cells were serially diluted 2-fold. Data points represent the mean ± standard deviation from three replicates. The most effective pairs were determined by the highest dilution of the OD₄₅₀ values.</p

Characteristics of MAbs against the NS1 protein of WNV.

a<p>Purified recombinant WNV-NS1 protein and DENV-NS1 were used as the coating antigen and were reacted with purified MAbs against the NS1 protein. The absorbance was measured at 450 nm: +, OD₄₅₀ = 0.5 to 1; ++, OD₄₅₀ = 1 to 2; +++, OD₄₅₀ = 2 to 3; OD₄₅₀>3.</p>b<p>The reactivity of each MAb was determined by IFA with WNV, DENVs, YFV, and JEV, and their IFA reactivity with normal cells were negative (data not shown). −, no reactivity; +, weak reactivity; ++, intermediate reactivity; +++, strong reactivity.</p>c<p>Not detected.</p><p>Characteristics of MAbs against the NS1 protein of WNV.</p

Additional file 2: of Changes in enterovirus serotype constituent ratios altered the clinical features of infected children in Guangdong Province, China, from 2010 to 2013

Demographical and clinical information of HFMD/HA patients admitted to Zhujiang hospital between 2010-2013. (XLS 4462 kb