Animal experiment diagram from the first day of immunization.

A) Experiment 1, B) Experiment 2. Numbers indicate weeks after the first immunization.</p

Western blot results.

(PDF)</p

SDS-PAGE (7%) analysis of monoclonal anti-MSTN antibody (mAb), egg yolk IgY and serum in non-reduced condition.

(PDF)</p

Bodyweights of chicks from the Control, chMSTN, and Myo2 groups in Experiment 2.

Bodyweights of chicks from the Control, chMSTN, and Myo2 groups in Experiment 2.</p

Hen-day egg production (A) and egg weight (B) in Experiment 2.

Hen day egg production was calculated by dividing the number of eggs produced within a week by the average number of hens on hand for the same time interval during the 5 weeks. Data were analyzed by one-way ANOVA. Means were compared by Tukey’s-HSD test. ***, P<0.001.</p