DataSheet_1_Derivation of predicted no-effect concentrations for thirty-five pharmaceuticals and personal care products in freshwater ecosystem.xlsx

Pharmaceuticals and personal care products (PPCPs) are contaminants of emerging concern. PPCPs have been detected in various environmental matrices, posing potential threat to human health and environment quality. Thus far, there are no water quality guidelines (WQGs) established for PPCPs for the protection of freshwater organisms and ecosystems. In this study, we used the species sensitivity distribution (SSD) and assessment factor (AF) approaches to derive the 5% hazardous concentrations (HC5) of 35 selected PPCPs using acute and chronic toxicity data. The predicted no-effect concentrations (PNECs) and the acute-to-chronic ratios (ACRs) of chemicals were inherently computed to support the derivation of WQGs and for ecological risk assessment. Among these, endocrine-disrupting chemicals and antipsychotics were shown to pose a greater threat to the freshwater environment and organisms. The highest PNEC was recorded for chloramphenicol (3,620 μg/L) and the lowest for fluoxetine (0.0000291 μg/L), which could have significant ecological risks. In addition, the commonly used default ACRs do not seem to provide adequate support for the prediction of chronic toxicity thresholds and WQGs, as the highest ACRs of these drugs reached 39,100 (e.g., chloramphenicol). The findings of this study provide critical scientific information regarding the development of WQGs for environmental management and the risk control of PPCPs.</p

DataSheet_1_Temperature-dependent effects of neonicotinoids on the embryonic development of zebrafish (Danio rerio).docx

The agricultural use of neonicotinoids is increasing worldwide and poses a threat to non-target organisms. The existing toxicity data of neonicotinoids that is mainly focused on widely used neonicotinoids ignores the influence of environmental factors, like temperature, related to climate changes, etc. To fill this data gap, the present study assessed the temperature-dependent toxicity of six neonicotinoids at four temperatures. Briefly, a fish embryo toxicity test was performed at the following temperatures—20, 23, 28, and 33°C—on zebrafish embryos to evaluate the lethal and sublethal effects of these toxicants. At 28°C, the lethal toxicity (LC50) values for these toxicants were cycloxaprid—3.36 mg/L, nitenpyram—7.08 mg/L, paichongding—17.2 mg/L, imidaclothiz—738.6 mg/L, dinotefuran—2,096 mg/L, and thiamethoxam—4,293 mg/L, respectively. Among the sublethal effects, the enzymatic activities changed significantly in neonicotinoid treatments, which revealed oxidative stress, metabolic disorders, and neurotoxicity. Particularly, acetylcholinesterase inhibition and glutathione S-transferase activation showed a significant dose–response relationship. However, cycloxaprid, nitenpyram, and paichongding were found to be more potent compared with imidaclothiz and thiamethoxam. The influence of temperature on these neonicotinoids demonstrated an inverted V-shaped relationship, in which toxicity decreased with the increase of temperature and then increased with the increase of temperature after exceeding the optimum temperature. This study provides a reference for the multiscale effects and potential mechanisms of neonicotinoids. Temperature-dependent toxicity is of great significance for future toxicity testing and risk assessment of chemicals in the face of global climate changes.</p