卵アレルギー患者血清によるIgE結合卵白抗原の検索 : ニワトリ,ウズラ,アヒル卵の主要タンパク質について

IgE binding antigen of the major proteins in egg white, which is hen (H), quail (Q) and duck (D) was determined using the sera of patients with egg allergy. The major proteins of egg white were ovotransferrin (OT), ovoinhibitor (OI), ovalbumin (OA), ovomucoid (OM) and lysozyme (LY). The age of egg allergy patients is used this present studey were 3 years 4 months-old to 4 months-old (RAST 2～6). After SDS-PAGE analysis of egg whites, Western-blotting and enzyme-immunoassay was examined using the serum of 33 patoents with egg allergy. The positive blots of IgE binding proteins were ranked score 1～5 by positive color. The blots from score 2 to score 5 were positive of IgE binding. The positive percent of IgE blotting to hen OM was shown to have higher positive percent relatively to the other major proteins with the serum of 33 patients. And, on the sera of RAST 3 and RAST 4～6, ratio of IgE binding to hen OM indicated high positive percent. Therefore, from this study, these findings suppose that hen OM is the most important allergen of egg white proteins

加工操作による卵白抗原・オボムコイドの低減化に及ぼす影響

In most cases, egg allergy is caused by ovomucoid (OM) in egg white. OM is said as allergen of egg whites. Determine the quantity of OM in processed foods containing egg is important for allergy patients. Antigen OM in processed foods of egg were determined as the amount of the soluble OM by ELISA using OMmAb (monoclonal antibody 4-8D). Processing operations were used that baked (170℃), steamed (98℃) and high-pressure-treated (686Mpa) to egg whites. Antigenicty of steamed OM decreased than non-heating OM. Antigenicity of baked and steamed egg white decreased than non-heating egg white. Antigenicity of OM in baked egg-processed foods that were supplemented with wheat and milk decreased than non-heating egg. However, antigenicity of OM in baked egg-processed foods without other materials was remarkably weaker than that of non-heating egg

Molecular Biosensing Mechanisms in the Spleen for the Removal of Aged and Damaged Red Cells from the Blood Circulation

Heinz bodies are intraerythrocytic inclusions of hemichrome formed as a result of hemoglobin (Hb) oxidation. They typically develop in aged red cells. Based on the hypothesis that hemichrome formation is an innate characteristic of physiologically normal Hb molecules, we present an overview of our previous findings regarding the molecular instability of Hb and the formation of hemichrome, as well as recent findings on Heinz body formation within normal human erythrocytes. Human adult Hb (HbO2 A) prepared from healthy donors showed a tendency to produce hemichrome, even at close to physiological temperature and pH. Recent studies found that the number of Heinz bodies formed in red cells increased with increasing temperature when freshly drawn venous blood from healthy donors was subjected to mild heating above 37 °C. These findings suggest that Hb molecules control the removal of non-functional erythrocytes from the circulation via hemichrome formation and subsequent Heinz body clustering. In this review, we discuss the molecular biosensing mechanisms in the spleen, where hemichrome formation and subsequent Heinz body clustering within erythrocytes play a key role in the removal of aged and damaged red cells from the blood circulation

魚類アレルゲンの分離とその性質

Salt-soluble fractions (I, II) , an alkali-soluble fraction (III) and an acid-soluble fraction (IV) were separated from fresh Cod (Gadus macrocephalus). Allergenic activities of the I～IV fractions were measured using mAb parvalbumin (mAb PV) and sera of fish-allergy patients. 36kDa and 12.3kDa proteins of the fractions showed a positive reaction against mAb PV. It was presumed that the 12.3kDa protein was parvalbumin (PV) judging from references. However, a positive reaction was indicated only in the 36kDa protein of the III fraction in the IgE reaction with a fish-allergy patient serum. Therefore, it was presumed that the patient having the above serum developed an allergy to a protein being different from PV. Moreover, it was presumed that the 36kDa allergen has the immunity intersection to PV because the positive reaction was shown against mAb PV. Allergenic activities of alkali-soluble fractions (III) from Salmon, (Oncorhynchus keta), Saury, (Cololabis saira), Sardine (Sardinops melanostictus), Flatfish (Pleuronectes herzensteini) were measured with mAb PV and the sera of the patients. However, a clear allergen was not observed as compared with the Cod III fraction. The Cod III fraction in the acidic solution (pH2～6) was decreased about 50% binding activity with mAb PV by heating at 100℃. The activity of the same fraction in the alkaline solution (pH7～10) was not decreased. Therefore, the Cod III fraction was considered to be heat-stable in the alkaline solution

Efficacy of carbon-ion radiotherapy and high-dose chemotherapy for patients with unresectable Ewing\u27s sarcoma family of tumors

Background Treatment for unresectable Ewing\u27s sarcoma family of tumors (ESFT) is a formidable challenge because of its high tendency for local and distant failure. Recently, carbon-ion radiotherapy (CIRT) has been applied to unre- sectable bone and soft tissue sarcoma. Additionally, high- dose chemotherapy (HDC) with stem cell rescue has been used to improve the survival of patients with relapsed ESFT. Here we report our experience with CIRT and HDC in the treatment of unresectable ESFT.\nMethods Five unresectable ESFT patients including 4 who underwent CIRT and HDC and one who underwentCIRT from 1999-2009 were retrospectively studied. After neoadjuvant chemotherapy, CIRT was conducted at the National Institute of Radiological Sciences in Chiba as local therapy. Consecutively, we employed HDC including busulfan, melphalan, and thiotepa with stem cell rescue.\nResults Two patients showed tumor shrinkage afterCIRT, including 1 patient who achieved partial response