Contribution of light and electron microscopy in the identification of morphological alterations in large Japanese field mouse (Apodemus speciosus) testes exposed to low-dose-rate radiations

Ionizing radiation affects biological systems, resulting in an increased risk of cancer and mutagenesis. Male reproductive function is sensitive to ionizing radiation, with implications connected to infertility. Following the Nuclear Power Plants accident of Fukushima in 2011, there was great attention regarding exposure damage to low-dose-rate (LDR) radiation on the reproductive system. This preliminary study aimed to evaluate the role of light (LM) and transmission electron microscopies (TEM) to identify the potential effects of LDR radio-exposure on the morphology of large Japanese field mouse (Apodemus speciosus) testes living in the Fukushima Daiichi Nuclear Power Plant (FDNPP) ex-evacuation area. After collection samples were subjected to the standard preparative for LM and TEM. The testicular parenchyma was characterized by numerous seminiferous tubules, delimited by a thick and continuous basal lamina. Basally, the germinal epithelium presented round and pale spermatogonia, primary spermatocytes; while, more adluminally, round and elongated spermatids were at different phases of development. Pale and irregular Sertoli cells were interspersed among germ cells. Occasionally, cytoplasmatic holes interrupted the nuclear membrane integrity in spermatocytes and spermatids. Residual bodies were seen at the luminal surface. In conclusion, this study suggests that LM and TEM analysis are useful in evaluating potential morphological features in the male reproductive system after LDR exposure

Witnessing the Hierarchical Assembly of the Brightest Cluster Galaxy in a Cluster at z=1.26

We have obtained a new high-resolution K'-band image of the central region of the rich X-ray cluster RX J0848.9+4452 at z=1.26. We found that the brightest cluster galaxy (BCG) in the cluster is clearly separated into two distinct objects. Whereas the optical to near-infrared colors of the objects are consistent with the predictions of passive evolution models for galaxies formed at high redshift, the luminosities of the two galaxies are both considerably fainter than predicted by passive evolution of BCG's in low and intermediate redshift clusters. We argue that this is evidence of an on-going merger of normal cluster ellipticals to form the dominant galaxy in the core of RX J0848.9+4452. The two galaxies appear to point towards the nearby cluster ClG J0848+4453 and are aligned with the outer X-ray contour of their parent cluster, supporting a model of BCG formation by collimated infall along the surrounding large-scale structure.Comment: 14 pages, 5 figures, to be appeared in ApJ Letter

Analysis of Radioactive Elements in Testes of Large Japanese Field Mice Using an Electron Probe Micro-Analyser after the Fukushima Accident

The Fukushima Daiichi nuclear power plant (FDNPP) accident drew global attention to the health risks of radiation exposure. The large Japanese field mice (Apodemus speciosus) are rodents endemic to, and distributed throughout, Japan. This wild rodent live in and around the ex-evacuation zone on the ground surface and/or underground. In this study, we evaluated the effect of chronic radiation exposure associated with FDNPP accident on the testes of large Japanese field mice. Morphological analysis and electron-prove X-ray microanalysis (EPMA) was undertaken on the testes. Morphological analysis of testes based on H&E staining showed that the spermatogenesis was observed normally in the breeding season of wild mice in the heavily contaminated area. However, caesium (Cs) was not detected in all testes of wild mice from FDNPP ex-evacuation zone. In conclusion, even if the testes and the process of spermatogenesis are hypersensitive to radiation, we could not detect radiation effects on the spermatogenesis and Cs in the examined large Japanese field mice testes following chronic radiation exposure associated with the FDNPP accident

Prospectively Isolated Cancer-Associated CD10+ Fibroblasts Have Stronger Interactions with CD133+ Colon Cancer Cells than with CD133− Cancer Cells

Although CD133 has been reported to be a promising colon cancer stem cell marker, the biological functions of CD133+ colon cancer cells remain controversial. In the present study, we investigated the biological differences between CD133+ and CD133− colon cancer cells, with a particular focus on their interactions with cancer-associated fibroblasts, especially CD10+ fibroblasts. We used 19 primary colon cancer tissues, 30 primary cultures of fibroblasts derived from colon cancer tissues and 6 colon cancer cell lines. We isolated CD133+ and CD133− subpopulations from the colon cancer tissues and cultured cells. In vitro analyses revealed that the two populations showed similar biological behaviors in their proliferation and chemosensitivity. In vivo analyses revealed that CD133+ cells showed significantly greater tumor growth than CD133− cells (P = 0.007). Moreover, in cocultures with primary fibroblasts derived from colon cancer tissues, CD133+ cells exhibited significantly more invasive behaviors than CD133− cells (P<0.001), especially in cocultures with CD10+ fibroblasts (P<0.0001). Further in vivo analyses revealed that CD10+ fibroblasts enhanced the tumor growth of CD133+ cells significantly more than CD10− fibroblasts (P<0.05). These data demonstrate that the in vitro invasive properties and in vivo tumor growth of CD133+ colon cancer cells are enhanced in the presence of specific cancer-associated fibroblasts, CD10+ fibroblasts, suggesting that the interactions between these specific cell populations have important roles in cancer progression. Therefore, these specific interactions may be promising targets for new colon cancer therapies

Concurrent Misclassification and Out-of-Distribution Detection for Semantic Segmentation via Energy-Based Normalizing Flow

Recent semantic segmentation models accurately classify test-time examples that are similar to a training dataset distribution. However, their discriminative closed-set approach is not robust in practical data setups with distributional shifts and out-of-distribution (OOD) classes. As a result, the predicted probabilities can be very imprecise when used as confidence scores at test time. To address this, we propose a generative model for concurrent in-distribution misclassification (IDM) and OOD detection that relies on a normalizing flow framework. The proposed flow-based detector with an energy-based inputs (FlowEneDet) can extend previously deployed segmentation models without their time-consuming retraining. Our FlowEneDet results in a low-complexity architecture with marginal increase in the memory footprint. FlowEneDet achieves promising results on Cityscapes, Cityscapes-C, FishyScapes and SegmentMeIfYouCan benchmarks in IDM/OOD detection when applied to pretrained DeepLabV3+ and SegFormer semantic segmentation models.Comment: Accepted to UAI 2023. Preprin

Assessment of interfractional prostate motion in patients immobilized in the prone position using a thermoplastic shell.

The aim of this study was to evaluate the interfractional prostate motion of patients immobilized in the prone position using a thermoplastic shell. A total of 24 patients with prostate calcifications detectable using a kilo-voltage X-ray image-guidance system (ExacTrac X-ray system) were examined. Daily displacements of the calcification within the prostate relative to pelvic bony structures were calculated by the ExacTrac X-ray system. The average displacement and standard deviation (SD) in each of the left-right (LR), anterior-posterior (AP), and superior-inferior (SI) directions were calculated for each patient. Based on the results of interfractional prostate motion, we also calculated planning target volume (PTV) margins using the van Herk formula and examined the validity of the PTV margin of our institute (a 9-mm margin everywhere except posteriorly, where a 6-mm margin was applied). In total, 899 data measurements from 24 patients were obtained. The average prostate displacements ± SD relative to bony structures were 2.8 ± 3.3, -2.0 ± 2.0 and 0.2 ± 0.4 mm, in the SI, AP and LR directions, respectively. The required PTV margins were 9.7, 6.1 and 1.4 mm in the SI, AP and LR directions, respectively. The clinical target volumes of 21 patients (87.5%) were located within the PTV for 90% or more of all treatment sessions. Interfractional prostate motion in the prone position with a thermoplastic shell was equivalent to that reported for the supine position. The PTV margin of our institute is considered appropriate for alignment, based on bony structures