Data_Sheet_1_A novel Toxoplasma gondii TGGT1_316290 mRNA-LNP vaccine elicits protective immune response against toxoplasmosis in mice.ZIP

Toxoplasma gondii (T. gondii) can infect almost all warm-blooded animals and is a major threat to global public health. Currently, there is no effective drug or vaccine for T. gondii. In this study, bioinformatics analysis on B and T cell epitopes revealed that TGGT1_316290 (TG290) had superior effects compared with the surface antigen 1 (SAG1). TG290 mRNA-LNP was constructed through the Lipid Nanoparticle (LNP) technology and intramuscularly injected into the BALB/c mice, and its immunogenicity and efficacy were explored. Analysis of antibodies, cytokines (IFN-γ, IL-12, IL-4, and IL-10), lymphocytes proliferation, cytotoxic T lymphocyte activity, dendritic cell (DC) maturation, as well as CD4+ and CD8+ T lymphocytes revealed that TG290 mRNA-LNP induced humoral and cellular immune responses in vaccinated mice. Furthermore, T-Box 21 (T-bet), nuclear factor kappa B (NF-kB) p65, and interferon regulatory factor 8 (IRF8) subunit were over-expressed in the TG290 mRNA-LNP-immunized group. The survival time of mice injected with TG290 mRNA-LNP was significantly longer (18.7 ± 3 days) compared with the survival of mice of the control groups (p 7) of mice immunized with TG290 mRNA-LNP significantly prolonged the survival time of these mice. This study demonstrates that TG290 mRNA-LNP induces specific immune response against T. gondii and may be a potential toxoplasmosis vaccine candidate for this infection.</p

Motion reversal stimulation.

<p>(A) A Newton's ring based stimulator. (B) The motion reversal procedure of the rings. It was illustrated with a 14 Hz motion reversal frequency and each reversal contained 7 frames. The phase of the Newton's ring was modulated by a sinusoid of 7 Hz in [0, π] to produce motion reversals.</p

Schematic diagram of the experimental setup.

<p>(A) Distribution of four stimulators on the computer screen. The cross indicating the center of the monitor was not presented on the screen. (B) The timing of the experimental sequence and behavioral task. For each subject, four experimental tasks were imposed.</p

Image plot of amplitude spectra of SSMVEPs (<i>f</i>₁

<p>–<b><i>f</i></b><b>₄) on Oz site.</b> Consecutive non-overlapped trials were sorted by ordinal run order from subject S1 to subject S6.</p

Offline highest ITRs on optimal time window length.

<p>Offline highest ITRs on optimal time window length.</p

Offline detection accuracies on fixed time window length (4 s).

<p>The asterisk (*) indicates statistically low accuracies of subject S6 as assessed by one-way ANOVA (<i>p</i> = 0.01).</p

Time series and Topographies from eleven-channel mean SSMVEPs of subject S1.

<p>(A) Time series of the mean SSMVEPs. Topographic distributions exhibited symmetrical activations of the transient P1, N2 and P2 components. (B) Spectral topographies of three predominant frequency components of the mean SSMVEPs. The most activation area was also symmetrically located on PO7, Oz and PO8 sites.</p

Additional file 2: Table S1. of Chidamide in relapsed or refractory peripheral T cell lymphoma: a multicenter real-world study in China

Patients’ baseline characteristics (DOCX 16 kb

Additional file 1: of Chidamide in relapsed or refractory peripheral T cell lymphoma: a multicenter real-world study in China

Methods (DOCX 16 kb