Clinical application of superselective transarterial embolization of renal tumors in zero ischaemia robotic-assisted laparoscopic partial nephrectomy

ObjectiveTo assess the feasibility and safety of zero ischaemia robotic-assisted laparoscopic partial nephrectomy (RALPN) after preoperative superselective transarterial embolization (STE) of T1 renal cancer.MethodsWe retrospectively analyzed the data of 32 patients who underwent zero ischaemia RALPN after STE and 140 patients who received standard robot-assisted laparoscopic partial nephrectomy (S-RALPN). In addition, we selected 35 patients treated with off-clamp RALPN (O-RALPN) from September 2017 to March 2022 for comparison. STE was performed by the same interventional practitioner, and zero ischaemia laparoscopic partial nephrectomy (LPN) was carried out by experienced surgeon 1-12 hours after STE. The intraoperative data and postoperative complications were recorded. The postoperative renal function, routine urine test, urinary Computed Tomography (CT), and preoperative and postoperative glomerular filtration rate (GFR) data were analyzed.ResultsAll operations were completed successfully. There were no cases of conversion to opening and no deaths. The renal arterial trunk was not blocked. No blood transfusions were needed. The mean operation time was 91.5 ± 34.28 minutes. The mean blood loss was 58.59 ± 54.11 ml. No recurrence or metastasis occurred.ConclusionFor patients with renal tumors, STE of renal tumors in zero ischaemia RALPN can preserve more renal function, and it provides a safe and feasible surgical method

The cause analysis of benign uretero-ileal anastomotic stricture after radical cystectomy and urinary diversion

BackgroundBenign uretero-ileal anastomotic stricture (UIAS) is a major complication following radical cystectomy (RC) and ileal orthotopic bladder substitution, and it can occur in combination with other complications. But risk factors for patients with UIAS have not been well described.Material and methodsWe retrospectively reviewed 198 patients treated with RC for bladder cancer from 2014 to 2019 at the Zhejiang Provincial People’s Hospital. Patient demographic and clinical variables were examined to determine the risk factors associated with UIAS by univariate and multivariate logistic regression analysis.ResultsA total of 180 patients into the group standards and in all 360 uretero-ileal anastomoses. Among the above cases, 22 patients developed UIAS, including 10 cases of left UIAS, nine cases of right UIAS, and three cases of bilateral UIAS. There was no difference in demographic, operative, or perioperative variables between patients with and without UIAS. In a multivariate analysis, after adjusting for gender, age, surgical methods, and underlying diseases, intraoperative or postoperative blood transfusion (HR = 0.144, P <0.01), postoperative urinary tract infection (HR = 3.624, P <0.01), and extracorporeal bladder anastomosis (HR = 3.395, P = 0.02) significantly increased the risk of UIAS.ConclusionsIn our experience, intraoperative or postoperative blood transfusion, postoperative urinary tract infection, and extracorporeal neobladder anastomoses increased the risk of UIAS after radical cystectomy and ileal orthotopic bladder substitution surgery. Further studies with larger samples are necessary to validate this result

Simulation of Swanson's literature-based discovery: anandamide treatment inhibits growth of gastric cancer cells in vitro and in silico.

Swanson's literature-based discovery focus on resurrecting previously published but neglected knowledge. In this study, we propose a two-step model of the discovery process and generate a hypothesis between anandamide and gastric cancer. Further, the potential relationship was confirmed by follow-up experimentation. The anandamide treatment resulted in cell cycle redistribution of gastric cancer cells. Most importantly, the variation of cell cycle was mediated by some genes from the B-terms of the closed discovery, indicating the potential role of the B-terms. Swanson's literature-based discovery not only collates data for possible interactions, but also provides the potential to observe the larger background behind these direct links and is an invaluable discovery tool for investigators

The B-term genes make up potential common molecular pathways between gastric cancer and anandamide.

<p>The B-term genes make up potential common molecular pathways between gastric cancer and anandamide.</p

The effect of anandamide on cell cycle regulators.

<p>A. Analysis of differential gene expression profiling in the AGS cells with the presence and absence of anandamide. Data were obtained from three samples each group. The differential gene with average Changes(fold >2) after exposure to anandamide (10 µM) for 24 h were picked up, the graphs show the fold changes of these cell cycle related genes. B. Effect of anandamide on the expression of the cell cycle regulators. Cells were harvested after anandamide (10 µM) or ethanol solvent treatment for 24h and analyzed by western blotting with antibodies against Chk1, p-Chk1, CDC25A, and CyclinB1. C. The results of western blotting were normalized against β-actin. Fold changes of these proteins were determined by comparison with levels measured in control ethanol treated cells. Data are presented as mean ± SD (n  =  3), * indicates <i>p<0.05</i> compared with control.</p

The predicted relationship between gastric cancer and anandamide.

<p>The predicted relationship between gastric cancer and anandamide.</p

The top-level MeSH categories of the stop-terms list.

<p>The top-level MeSH categories of the stop-terms list.</p

The effect of anandamide on gastric cancer cells.A.The structures of tetrahydrocannabinol and anandamide.

<p>Anandamide functional groups include amides, esters, and ethers of long-chain polyunsaturated fatty acids, and structurally share critical pharmacophores with D-9-tetrahydrocannabinol (THC). B.Anandamide treatment inhibits the proliferation of gastric cancer cells. BGC823, SGC7901, AGS, and N87 cells were treated with synthetic anandamide at concentrations of 0, 50, and 100 µM for 24 h. The proliferation rate was detected by the MTT assay. Cells treated with vehicle of ethanol were employed as a control. Data are presented as mean ± SD (n = 3), * indicates <i>p<0.01</i> compared with control. C.Anandamide treatment inhibited the proliferation of AGS and N87 cells. AGS and N87 cells were treated with synthetic anandamide with increasing concentrations of 0, 0.01, 0.1, 1, and 10 µM for 24 h. The proliferation rate was detected by MTT assay. Data are presented as mean ± SD (n = 3). * indicates <i>p<0.01</i> compared with control. D.The effect of anandamide on apoptosis in AGS and N87 cells. AGS and N87 cells were treated with anandamide at a concentration of 10 µM for 24 h. Apoptosis were analyzed by flow cytometry. Cells treated with vehicle were used as a control.</p

The top 10 MeSH terms of 2 topics—gastric cancer (A) and psychology (B).

<p>The top 10 MeSH terms of 2 topics—gastric cancer (A) and psychology (B).</p

The 6 categories of the target profile C.

<p>The 6 categories of the target profile C.</p