Reasons for failure of web-based application IT projects – An Empirical Study in Malaysia

Setiap tahun, organisasi rugi berjuta-juta ringgit kerana aplikasi berasaskan sesawang teknologi maklumat projek gagal. Every year, organizations lose millions of dollars due to failure of web-based application information technology (IT) projects

Reasons for failure of web-based application IT projects – An Empirical Study in Malaysia

Setiap tahun, organisasi rugi berjuta-juta ringgit kerana aplikasi berasaskan sesawang teknologi maklumat projek gagal. Every year, organizations lose millions of dollars due to failure of web-based application information technology (IT) projects

Heterologous expression of cytotoxic sesquiterpenoids from the medicinal mushroom Lignosus rhinocerotis in yeast

Background: Genome mining facilitated by heterologous systems is an emerging approach to access the chemical diversity encoded in basidiomycete genomes. In this study, three sesquiterpene synthase genes, GME3634, GME3638, and GME9210, which were highly expressed in the sclerotium of the medicinal mushroom Lignosus rhinocerotis, were cloned and heterologously expressed in a yeast system. Results: Metabolite profile analysis of the yeast culture extracts by GC-MS showed the production of several sesquiterpene alcohols (C 15 H 26 O), including cadinols and germacrene D-4-ol as major products. Other detected sesquiterpenes include selina-6-en-4-ol, ß-elemene, ß-cubebene, and cedrene. Two purified major compounds namely (+)-torreyol and a-cadinol synthesised by GME3638 and GME3634 respectively, are stereoisomers and their chemical structures were confirmed by 1 H and 13 C NMR. Phylogenetic analysis revealed that GME3638 and GME3634 are a pair of orthologues, and are grouped together with terpene synthases that synthesise cadinenes and related sesquiterpenes. (+)-Torreyol and a-cadinol were tested against a panel of human cancer cell lines and the latter was found to exhibit selective potent cytotoxicity in breast adenocarcinoma cells (MCF7) with IC 50 value of 3.5 ± 0.58 µg/ml while a-cadinol is less active (IC 50 = 18.0 ± 3.27 µg/ml). Conclusions: This demonstrates that yeast-based genome mining, guided by transcriptomics, is a promising approach for uncovering bioactive compounds from medicinal mushrooms

Reasons for the Failure of Web-Based Application of Information Technology Projects: An Empirical Study in Malaysia

Every year, organizations lose millions of dollars due to failure of web-based application of information technology (IT) projects. Research shows that companies have difficulties in web-based application IT projects to complete on time or on budget or within the scope and any of these combinations. The purpose of this study is to identify the factors influencing failure of web-based application of IT projects, particularly in the context of Malaysia. The study was conducted on 155 experieced project managers in handling web-based IT projects. The top ten failure factors were identified. The findings of the study reveal that lack of clarity of goals, low teamwork quality, ineffective project management, no reward and recognition system in place and insufficient resources are the core factors for the failure of web-based application of IT projects. The significance of this research lies in the fact that its results will add new knowledge in the project management field by identifying the relative importance of the factors that have an impact on web-based application of IT project failure